A5091027109- Antibiotic Resistance in Bacteria
- Escherichia coli research studies
- Pharmaceutical and Antibiotic Environmental Impacts
- Antimicrobial Resistance in Staphylococcus
- Antibiotics Pharmacokinetics and Efficacy
- Mycobacterium research and diagnosis
- Salmonella and Campylobacter epidemiology
- Clostridium difficile and Clostridium perfringens research
- Pneumonia and Respiratory Infections
- Bacteriophages and microbial interactions
Public Health Agency of Canada
2025
University of Guelph
2015-2024
Historically, extended-spectrum cephalosporin resistance in bacteria from animals Canada has been attributed to the SHV and CMY β-lactamase families. This pattern is beginning change with emergence of blaCTX-M gene family among Escherichia coli recovered various animal species. Here we analyze compare whole genome sequences blaCTX-M-positive E. isolates (n = 173) dogs, chicken, swine, horses beef cattle Canada. Ten variants were identified blaCTX-M-1,-14, -15, -27 blaCTX-M-55 being most...
Abstract Extended-spectrum cephalosporins (ESCs) are critically important antimicrobial agents for human and veterinary medicine. ESC resistance (ESC-R) genes have spread worldwide through plasmids clonal expansion, yet the distribution dynamics of ESC-R in different ecological compartments poorly understood. Here we use whole genome sequence data Enterobacterales isolates animal origin from Europe North America identify contrasting temporal dynamics. AmpC β-lactamases were initially more...
Extended-spectrum cephalosporins (ESCs) are third and fourth generation cephalosporin antimicrobials used in humans animals to treat infections due multidrug-resistant (MDR) bacteria. Resistance ESCs (ESC-R) Enterobacterales is predominantly the production of extended-spectrum β-lactamases (ESBLs) plasmid-mediated AmpC (AmpCs). The dynamics ESBLs AmpCs changing across countries host species, result global transmission ESC-R genes. Plasmids known play a key role this dissemination, but...
Ciprofloxacin is important for treatment of severe or invasive Salmonella infections in humans. As laboratories transition from phenotypic to genomics-based methods determining ciprofloxacin non-susceptibility, it define the correlation between genetic determinants resistance and outcomes. Here, we examined mechanisms tested hypothesis that isolates containing only one mechanism had intermediate while two more full according breakpoints Clinical Laboratory Standards Institute. Among 13,750...
The goal of this study was to determine the frequency resistance extended-spectrum cephalosporins (ESCs) in Escherichia coli and other Enterobacterales from turkeys Canada characterize associated determinants. Pooled fecal samples were collected 77 turkey farms across British Columbia, Québec, Ontario. Isolates obtained with without selective enrichment cultures compared isolates diagnostic submissions suspected colibacillosis cases identified using MALDI-TOF susceptibility ESCs assessed by...
Conserved IncI1 and IncHI1 plasmids carrying bla CTX-M-1 have been found circulating in chickens horses from continental Europe, respectively. In Canada, is overwhelmingly the most common CTX-M variant Escherichia coli chicken can be recovered at lower frequencies swine, cattle, dogs. Whole-genome sequencing has identified a large genetic diversity of isolates this variant, warranting further investigations into gene. Therefore, objective study was to describe profiles E . Canadian domestic...
A recent preliminary study from our group found that extended-spectrum cephalosporin-resistance determinants can be detected in the majority of composite fecal samples collected Alberta feedlot cattle. Most notably, blaCTX-M genes were 46.5% samples. Further isolate characterization identified blaCTX-M-15 and blaCTX-M-27, which are widespread bacteria humans. We hypothesized Escherichia coli human beef cattle origins share same pool genes. In this study, we aimed to assess compare genomic...