A5014996359- MicroRNA in disease regulation
- Ubiquitin and proteasome pathways
- RNA Research and Splicing
- RNA modifications and cancer
- Adipokines, Inflammation, and Metabolic Diseases
- Cancer-related molecular mechanisms research
- Genomics and Chromatin Dynamics
- Adipose Tissue and Metabolism
- RNA Interference and Gene Delivery
- Cellular Mechanics and Interactions
- Infective Endocarditis Diagnosis and Management
- Autophagy in Disease and Therapy
- NF-κB Signaling Pathways
- Retinoids in leukemia and cellular processes
- Brain Metastases and Treatment
- Neonatal and Maternal Infections
- Cell death mechanisms and regulation
- Cellular transport and secretion
- Animal Genetics and Reproduction
- IL-33, ST2, and ILC Pathways
- Cancer Cells and Metastasis
- Streptococcal Infections and Treatments
- Immune Cell Function and Interaction
- Breast Cancer Treatment Studies
- Immune Response and Inflammation
Wenzhou Medical University
2025
Kiang Wu Nursing College of Macau
2025
Queen Elizabeth II Health Sciences Centre
2025
Shanghai Skin Disease Hospital
2025
Tongji University
2025
Government of Prince Edward Island
2025
Hunan Normal University
2013-2024
Changsha Normal University
2024
Beijing Tian Tan Hospital
2023
Capital Medical University
2022-2023
Aberrant expression of transcription factor AP-2α has been functionally associated with various cancers, but its clinical significance and molecular mechanisms in human glioma are largely elusive. Methods: was analyzed tissues by immunohistochemistry (IHC) cell lines Western blot. The effects on proliferation, migration, invasion tumor formation were evaluated the 3-(4,5-dimethyNCthiazol-2-yl)-25-diphenyltetrazolium bromide (MTT) transwell assays vitro nude mouse models vivo. influence...
Group 2 innate lymphoid cells (ILC2s) in white adipose tissue (WAT) promote WAT browning and assist preventing the development of obesity. However, how ILC2 is regulated remains largely unknown. Here, our study shows that ILC2s are present brown (BAT) as well subcutaneous epididymal (sWAT eWAT). The fractions ILC2s, natural killer T (NKT) eosinophils sWAT, eWAT BAT significantly decreased by high-fat-diet (HFD) feeding leptin deficiency-induced Consistent with this, expression circulating...
Highlights•mTORC1 is a potent regulator of white to beige adipogenesis•mTORC1 inhibition leads WAT browning and occurs despite sympathetic denervation•COX-2 prostaglandins are essential for in Raptor-deficient mice•mTORC1 suppresses COX-2/PG pathway via CRTC2-dependent mechanismSummaryBeige adipocytes present adipose tissue (WAT) have thermogenic capacity orchestrate substantial energy metabolism counteract obesity. However, adipocyte-derived signals that act on progenitor cells control...
The transcription factor AP-2α functions as a tumor suppressor by regulating various genes that are involved in cell proliferation and apoptosis. Chemotherapeutic drugs including cisplatin induce post-transcriptionally endogenous AP-2α, which contributes to chemosensitivity enhancing therapy-induced microRNAs (miRNAs) miR-200b, miR-200c miR-429 (miR-200b/200c/429) up-regulated endometrial esophageal cancers, their overexpression correlates with resistance treatment. Using computational...
ILC2s are present in adipose tissue and play a critical role regulating thermogenesis. However, the mechanisms underlying activation of adipose-resident remain poorly defined. Here, we show that IL-33, potent ILC2 activator, stimulates phosphorylation AMPK at Thr172 via TAK1 primary ILC2s, which provides feedback mechanism to inhibit IL-33-induced NF-κB IL-13 production. Treating with adiponectin or an receptor agonist (AdipoRon) activated decreased IL-33-NF-κB signaling. AdipoRon also...
Abstract Long noncoding RNAs (lncRNAs) play crucial roles in hepatocellular carcinoma (HCC). However, the underlying molecular mechanisms of small nucleolar RNA host gene 16 ( SNHG16 ) for regulating cell cycle and epithelial to mesenchymal transition (EMT) remain elusive. In this study, expression profiles HCC tissues or lines were compared with those normal hepatocyte line. The effect knockdown was investigated by using vitro loss‐of‐function experiments vivo nude mouse experiments....
Dysregulation of macroautophagy/autophagy is implicated in obesity and insulin resistance. However, it remains poorly defined how autophagy regulates adipocyte development. Using adipose-specific rptor/raptor knockout (KO), atg7 KO rptor double-KO mice, we show that inhibiting MTORC1 by RPTOR deficiency led to autophagic sequestration lipid droplets, formation LD-containing lysosomes, elevation basal isoproterenol-induced lipolysis vivo primary adipocytes. Despite normal differentiation at...
Curcumin, a phytochemical from rhizomes of the plant Curcuma longa, has been reported to exert potential anticancer properties in various cancer types, including acute myeloid leukemia (AML). However, underlying mechanism remains poorly understood. The present study demonstrated that curcumin had stronger cytotoxic activity against AML cells compared with three other types phytochemicals (epigallocatechin gallate, genistein and resveratrol). Protein phosphorylation profiling using an...
Abstract Programmed death-ligand 1 (PD-L1) ensures that tumor cells escape T-cell-mediated immune surveillance. However, gliomas are characteristic of the low response and high-resistance therapy, it is necessary to understand molecular regulatory mechanisms in glioblastoma, especially limited regulation PD-L1 expression. Herein, we show expression AP-2α correlated with high high-grade glioma tissues. binds directly promoter CD274 gene, not only inhibits transcriptional activity but enhances...
Abstract Anti‐cancer peptides (ACPs) represent a promising potential for cancer treatment, although their mechanisms need to be further elucidated improve application in therapy. Lycosin‐I, linear amphipathic peptide isolated from the venom of Lycosa singorensis , shows significant anticancer potential. Herein, it is found that which can self‐assemble into nanosphere structure, has multimodal mechanism action involving lipid binding selective and effective treatment leukemia....
Radiotherapy is one of the main treatment modalities for advanced hepatocellular carcinoma (HCC). Ferroptosis has been shown to promote radiosensitivity HCC cells, but it remains unclear whether epigenetic regulations function in this process. In study, we found that overexpression METTL3 was associated with poor prognosis. Knockdown promoted by inducing ferroptosis. Mechanistically, targeted adenine (+1795) on SLC7A11 mRNA, and m6A reader IGF2BP2 mRNA stability recognizing binding site....
Ciprofloxacin is important for treatment of severe or invasive Salmonella infections in humans. As laboratories transition from phenotypic to genomics-based methods determining ciprofloxacin non-susceptibility, it define the correlation between genetic determinants resistance and outcomes. Here, we examined mechanisms tested hypothesis that isolates containing only one mechanism had intermediate while two more full according breakpoints Clinical Laboratory Standards Institute. Among 13,750...
Hypertensive nephropathy (HN), caused by long-term poorly controlled hypertension, is the second common cause of end-stage renal disease after diabetes mellitus, but pathogenesis HN unclear. The purpose this study was to identify biological pathways involved in progression and bile acid (BA)-related biomarkers, analyze role acids HN. Download gene microarray data from Gene Expression Omnibus. Differentially expressed genes (DEGs) associated with were identified, then DEGs subjected Ontology...
Introduction. Invasive meningococcal disease (IMD) is a nationally notifiable illness in Canada due to its potential severity and transmissibility. Vaccination strategies differ by province/territory are informed changes the antigenic characteristics of circulating strains. Gap Statement. Though IMD statistics tracked at provincial/territorial level, there lack published data characterizing trends epidemiology this national level. Aim. To examine culture-confirmed during period 2015–2023....
Abstract AP‐2 is a transcription factor implicated in mammalian development, cell proliferation, apoptosis, and carcinogenesis. To identify potential AP‐2α‐interacting partners, yeast two‐hybrid screen was performed human brain cDNA library. One of the identified clones encodes potassium channel tetramerization domain‐containing 1 (KCTD1). We demonstrated novel KCTD1–AP‐2α interaction vitro by GST pull‐down assays vivo co‐immunoprecipitation mapped domains to N‐termini both proteins. In...
The role of Eps8 in human breast cancer was studied, and we found that overexpressed >60% samples compared with adjacent normal tissues by immunohistochemical analysis. highly expressed the invasive cell line MDA-MB‑231 weakly lines MCF7 MDA-MB‑468. stably expressing established G418 screening, ectopic expression enhanced growth survival as assessed MTT analysis, viability liquid colony formation, whereas lentiviral shRNA cells resulted a significant reduction cellular proliferation vitro...
BackgroundTumor necrosis factor α-induced protein 1 (TNFAIP1) is frequently downregulated in cancer cell lines and promotes apoptosis. However, its role, clinical significance molecular mechanisms hepatocellular carcinoma (HCC) are unknown.MethodsThe expression of TNFAIP1 HCC tumor tissues was measured by Western blot immunohistochemistry. The effects on proliferation, apoptosis, metastasis, angiogenesis formation were evaluated Cell Counting Kit-8 (CCK8), Terminal deoxynucleotidyl...
The intermittent fasting (IF) diet has profound benefits for diabetes prevention. However, the precise mechanisms underlying IF's beneficial effects remain poorly defined. Here, we show that expression levels of cyclooxygenase-2 (COX-2), an enzyme produces prostaglandins, are suppressed in white adipose tissue (WAT) obese humans. In addition, COX-2 WAT is markedly upregulated by IF mice. Adipocyte-specific depletion led to reduced fractions CD4+Foxp3+ Tregs and a substantial decrease...
The role of microRNAs (miRNAs) in regulating gene expression is currently an area intense interest. Previous studies have shown that miRNA-372 plays crucial roles gastric tumorigenesis by targeting the mRNA tumor necrosis factor, α-induced protein 1 (TNFAIP1). present study showed miR-373 upregulated adenocarcinoma tissue and carcinoma cell lines when compared to normal tissues. overexpression cancer cells increased proliferation. A bioinformatics search revealed a conserved target site...
As a member of the polymerase delta-interacting protein 1 (PDIP1) gene family, potassium channel tetramerisation domain-containing 10 (KCTD10) interacts with proliferating cell nuclear antigen (PCNA) and δ, participates in DNA repair, replication cell-cycle control. In order to further investigate physiological functions KCTD10, we generated KCTD10 knockout mice. The heterozygous KCTD10(+/-) mice were viable fertile, while homozygous KCTD10(-/-) showed delayed growth from E9.0, died at...
Transcription factor AP-2α is involved in many types of human cancers, but its role hepatocellular carcinogenesis largely unknown. In this study, we found that expression was low 40% cancers compared with adjacent normal tissues by immunohistochemical analysis. Moreover, or absent cancer cell lines (HepG2, Hep3B, SMMC-7721 and MHHC 97-H). Human liver Hep3B stably overexpressing were established lentiviral infection puromycin screening, the ectopic able to inhibit growth proliferation...
Transcription factor AP-2β plays an important role in human cancer, but its clinical significance hepatocellular carcinogenesis is largely unknown. Methods: expression was detected cancer (HCC) tissues and cell lines. The effects of on HCC proliferation, migration, invasion, tumor formation metastasis were evaluated by MTT, colony transwell assays vitro mouse experiments vivo. association between miR-27a/EMT markers lines analyzed. Results: decreased Reduced significantly associated with...
Small intestinal epithelium homeostasis involves four principal cell types: enterocytes, goblet, enteroendocrine and Paneth cells. Epidermal growth factor (EGF) has been shown to affect enterocyte differentiation. This study determined the effect of dietary EGF on differentiation in piglet small intestine potential mechanisms. Forty-two weaned piglets were used a 2 × 3 factorial design; major factors time post-weaning (days 7 14) treatment (0, 200 or 400 µg/kg supplementation). The numbers...