Bcr‑abl fusion transcripts, resulting from translocation t(9;22), are hallmarks of Philadelphia chromosome positive (Ph+) leukemias. This is detected in >90% patients with chronic myelogenous leukemia and ~20% acute lymphoblastic patients, which predominantly express the p210 p190 proteins, respectively. Although occurrence t(9;22) healthy individuals has been previously demonstrated, number studies limited results inconsistent. The present study screened for presence bcr‑abl transcripts...

Combinatorial therapeutic strategies to eradicate tumors can be superior a single modality. Docetaxel (DT) has been approved for the treatment of local or metastasized breast cancer alone in combination with other chemotherapeutic agents. Thymoquinone (TQ) originated from seeds Nigella Sativa plant reported possess vitro and vivo antitumor activity against variety tumors. In current study, we have investigated synergistic anticancer efficacy novel DT TQ on MCF7 cell line using MTT viability...

Pharmacognosy Magazine,2012,8,32,319-324.DOI:10.4103/0973-1296.103664Published:November 2012Type:Original ArticleAuthors:Rana Abu-Dahab, Fatma Afifi, Violet Kasabri, Lara Majdalawi, and Randa Naffa Author(s) affiliations:Rana Abu-Dahab1, Afifi1, Kasabri1, Majdalawi1, Naffa2 1Faculty of Pharmacy, The University Jordan, Amman, Jordan 2Faculty Medicine, Abstract:Background: antiproliferative activity Salvia species grown in has not been fully evaluated yet. aim this work was to study the crude...

A total of 100 Jordanian clinical Staphylococcus aureus isolates was analysed for the presence enterotoxin genes sea, seb, sec, sed and see using multiplex PCR. Twenty-three (23 %) were potentially enterotoxigenic. The prevalence sec sea plus among 15, 4 %, respectively. None harboured sed, seb or genes. S. subjected to DNA fingerprinting by randomly amplified polymorphic (RAPD) analysis test whether harbouring toxin genetically clustered. 13 genotypes identified at a 47 % similarity level....

The Review of Diabetic Studies,2017,14,4,364-371.DOI:10.1900/RDS.2017.14.364Published:February 2018Type:Original ArticleAuthors:Amal Akour, Violet Kasabri, Nailya Bulatova, Suha Al Muhaissen, Randa Naffa, Hiba Fahmawi, Munther Momani, Ayman Zayed, and Yasser Bustanji Author(s) affiliations:Amal Akour1, Kasabri1, Bulatova1, Muhaissen1, Naffa1, Fahmawi1, Momani2, Zayed2, Bustanji1 1School Pharmacy Medicine, the University Jordan, Amman, JORDAN. 2Endocrinology Diabetes Unit, Jordan Hospital,...

A series of new N1-(coumarin-7-yl)amidrazones incorporating N-piperazines and related congeners were synthesized by reacting the hydrazonoyl chloride derived from 7-amino-4-methylcoumarin with appropriate piperazines. The chemical structures newly prepared compounds supported elemental analyses, 1H-NMR, 13C-NMR, ESI-HRMS spectral data. antitumor activity was evaluated. Among all tested,...

Angiogenesis is one of cancer hallmarks that are required for both progression and metastasis. In this study we examined the antiangiogenic properties ethanolic crude extracts four Salvia species grown in Jordan.The direct activity was evaluated using various models: ex vivo rat aortic ring assay, vitro assessment HUVEC proliferation migration, CAM while used changes expression HIF-1α VEGF breast cells (MCF 7) as an indicative indirect activity.All showed a potential however two were found...

The purpose of the current study was to examine association serum copper and lipid concentrations with changes in cognitive function elderly Jordanian individuals. population consisted two groups: 52 dementia patients 50 co

Asthma and allergic rhinitis are respiratory diseases with a significant global burden. Forkhead box O3 (FOXO3) is gene involved in the etiology of number diseases. The objective this study to assess association rs13217795, an intronic FOXO3 single-nucleotide polymorphism, asthma rhinitis. In case–case–control genetic study, genotyping was conducted using PCR–RFLP method. Genotype-based associations were investigated under general, recessive, dominant models disease penetrance binomial...

Metastatic melanoma is the most aggressive type of skin cancer. Previously, we identified plasma membrane Ca2+ pump isoform 4b (PMCA4b or ATP2B4) as a putative metastasis suppressor in BRAF mutant cells. Metastasis suppressors are often downregulated cancer, therefore, it important to identify pathways involved their degradation. Here, studied role p38 MAPK PMCA4b degradation and its effect on metastasis. We found that activation induces internalization subsequent through endo/lysosomal...

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Background: The aim of the study was to compare and correlate glycated high-density lipoprotein (GHDL-C) low-density (GLDL-C) plasma levels with adiposity indices [weight/hip ratio (WHR) body index (BAI)], lipid ratios hematological [platelet/lymphocyte (PLR), monocyte/lymphocyte (MLR)]. Methods: This a cross-sectional 30 nondiabetic metabolic syndrome (MetS) patients, prediabetic or type 2 diabetes mellitus (T2DM) patients normoglycemic controls. Results: Remarkably both GHDL-C GLDL-C...

Introduction: Gasdermin A (GSDMA) and gasdermin B (GSDMB) have been associated with childhood, to a lesser extent adult, asthma in many populations. Aims: In this study, we investigated the association between GSDMA GSDMB variants incidence of adult childhood among Jordanians. Methods: Subjects were divided into two groups: adults children. Within group there 129 patients 111 healthy controls. pediatric 98 112 (rs7212938, T/G) (rs7216389, T/C) polymorphisms genotyped using PCR-RFLP method....

Oxytocin (OXT), fetuin-A and interleukin-18 (IL-18) are involved in the development progression of metabolic syndrome (MetS) prediabetes (pre/T2DM).This study aimed to compare correlate plasma levels OXT, IL-18 with clinical parameters, haematological indices adiposity Jordanian MetS subjects. In a cross-sectional study, 30 normoglycaemic lean participants (control), participants, 29 pre/T2DM were recruited.Median circulating both OXT lower versus control group. (pg/ml; median interquartile...

We demonstrated that the plasma membrane Ca2+ ATPase PMCA4b inhibits migration and metastatic activity of BRAF mutant melanoma cells. Actin dynamics are essential for cells to move, invade metastasize, therefore, we hypothesized affected cell through remodeling actin cytoskeleton. found expression in A375 induced a profound change shape, culture morphology, displayed polarized migratory character. Along with these changes became more rounded increased cell–cell connections, lamellipodia...

Objective: The present in vitro studies aimed to investigate the pancreatic and extrapancreatic effects of crude aqueous extracts (AE) Achillea santolina L, Eryngium creticum Lam, Pistacia atlantica Desf utilized Jordan diabetes ethnomedicine. Methods: Bioassays β-cell proliferation insulin secretion as well glucose diffusion possible modes action were recruited. Results: Similar L-alanine insulinotropic efficacy MIN6 β-cell, glucose-stimulated Ca2+ regulated- was potentiated by AEs...

<h3>Objectives:</h3> To investigate the frequencies of apolipoprotein E (APOE) alleles and genotypes study their relationship with lipid profile in Jordanian patients late-onset Alzheimer's disease (AD). <h3>Methods:</h3> This case-control was carried out on 71 individuals: 38 AD (age ≥65 years) 33 age-matched healthy controls. All participants were recruited from senior homes Jordan University Hospital, Amman, between January 2010 December 2013. Each sample examined for APOE's 3 major...

A new series of 6-substituted-4-methyl-3-(4-arylpiperazin-1-yl)cinnolines 8–10 were synthesized as potential antifungal agents via intramolecular cyclization the respective 1-(2-arylhydrazono)-1-(4-arylpiperazin-1-yl)propan-2-ones 5–7, mediated by polyphosphoric acid (PPA). The amidrazones themselves direct interaction appropriate hydrazonoyl chlorides 4a–d with corresponding N-substituted piperazine in presence triethylamine. structures prepared compounds confirmed elemental analyses,...

Angiogenesis is essential for the growth, invasion, and metastasis of most solid tumors has become a valuable pharmacological target cancer prevention treatment. This study was performed to assess antiangiogenic activity 31 medicinal plants grown sold in Jordan. The assessed using rat aortic ring assay. Out extracts, 15 extracts showed more than 50 % inhibition blood vessels outgrowth from primary tissue explants (p = 0.000). Three these potential cytotoxic effect on normal fibroblast cells....

A 49-y-old male with erythrodermic psoriasis developed persistent bacteraemia for 3 months due to methicillin-resistant Staphylococcus aureus despite antimicrobial therapy. The skin was the likely focus. Three consecutive isolates from blood and 1 nose were identical had vancomycin MIC of 4 mg/l.