A5057885904- Legume Nitrogen Fixing Symbiosis
- Mycorrhizal Fungi and Plant Interactions
- Plant and animal studies
- Plant Parasitism and Resistance
- Plant Virus Research Studies
- Fungal Biology and Applications
- Plant nutrient uptake and metabolism
- Microbial Community Ecology and Physiology
- Agronomic Practices and Intercropping Systems
- Nematode management and characterization studies
- Mycobacterium research and diagnosis
- Physiological and biochemical adaptations
- Biocrusts and Microbial Ecology
- Cassava research and cyanide
- Phytoplasmas and Hemiptera pathogens
- Antibiotic Resistance in Bacteria
- Environmental DNA in Biodiversity Studies
- Bacteriophages and microbial interactions
- Selenium in Biological Systems
- Plant and Fungal Species Descriptions
- Water Treatment and Disinfection
- Plant and Fungal Interactions Research
- Gut microbiota and health
- Wastewater Treatment and Nitrogen Removal
- Plant-Microbe Interactions and Immunity
ZheJiang Academy of Agricultural Sciences
2022-2024
Research Center for Eco-Environmental Sciences
2024
Chinese Academy of Sciences
2024
University of Chinese Academy of Sciences
2024
Huazhong Agricultural University
2007-2023
Norwich Research Park
2022
John Innes Centre
2022
Arbuscular mycorrhizas contribute significantly to inorganic phosphate (Pi) uptake in plants. Gene networks involved the regulation and function of Pht1 family transporters legume species during AM symbiosis are not fully understood. In order characterize six distinct members mycorrhizal Astragalus sinicus, we combined cellular localization, heterologous functional expression yeast with expression/subcellular localization studies reverse genetics approaches planta. Pht1;1 Pht1;4 silenced...
Abstract Many plants associate with arbuscular mycorrhizal fungi for nutrient acquisition, while legumes also nitrogen-fixing rhizobial bacteria. Both associations rely on symbiosis signaling and here we show that cereals can perceive lipochitooligosaccharides (LCOs) activation of signaling, surprisingly including Nod factors produced by However, stringent perception specifically decorated LCOs, is absent in cereals. LCO activated starvation, through transcriptional regulation Nodulation...
Gram-negative bacteria can produce outer membrane vesicles (OMVs), and most functional studies of OMVs have been focused on mammalian-bacterial interactions. However, research the rhizobia is still limited. In this work, we isolated purified from Sinorhizobium fredii HH103 under free-living conditions that were set as control (C-OMVs) symbiosis-mimicking induced by genistein (G-OMVs). The soybean roots treated with G-OMVs displayed significant deformation root hairs. significantly expression...
Lipopolysaccharide (LPS) is a ubiquitous microbial-associated molecular pattern. Plants can sense the three components of LPS, including core polysaccharide, lipid A, and O-antigen. LPS biosynthesis an essential factor for successful establishment symbiosis in rhizobium-legume plant system. The MCHK_1752 gene (Mesorhizobium huakuii 7653R gene) encodes O-antigen polymerase affects synthesis Here, we investigated symbiotic phenotypes six Astragalus sinicus accessions inoculated with deletion...
Chronic enteritis can produce an excess of reactive oxygen species resulting in cellular damage. Stanniocalcin-1(STC-1) reportedly possesses anti-oxidative activity, the aim this study was to define more clearly direct contribution STC-1 stress cattle. In study, primary intestinal epithelial cells (IECs) were exposed hydrogen peroxide (H2O2) for different time intervals mimic chronic enteritis-induced Prior treatment with 200 µM H2O2, transfected a recombinant plasmid 48 h over-express...
Recent research on abundant and rare bacteria has expanded our understanding of bacterial community assembly. However, the relationships with antibiotic resistance genes (ARGs) remain largely unclear. Here, we investigated biogeographical patterns assembly processes from river sediment at high altitudes (Lhasa River, China) their potential association ARGs. The results showed that were dominated by Proteobacteria (55.4%) Cyanobacteria (13.9%), while (33.6%) Bacteroidetes (18.8%) main...
Summary Visualization of root colonization by arbuscular mycorrhizal fungi (AMF) is the most elementary experiment in field symbiosis. The widely used approach for evaluating levels AMF staining with trypan blue or ink, which scored using time‐consuming grid intersection method. Here we demonstrate use an anthocyanin‐based visual marker system visualizing Medicago truncatula roots. Expression MtLAP1 , a transcription factor regulates production anthocyanins, from AMF‐induced Kunitz Protease...
Rhizobia can infect legumes and induce the coordinated expression of symbiosis defense genes for establishment mutualistic symbiosis. Numerous studies have elucidated molecular interactions between rhizobia host plants, which are associated with Nod factor, exopolysaccharide, T3SS effector proteins. However, there been relatively few reports about how plant recognizes outer membrane proteins (OMPs) to mediate symbiotic nodulation. In our previous work, a gene (Mhopa22) encoding an OMP was...
In the Rhizobium-Legume symbiosis, nodulation outer protein P (NopP) effector is one of key regulators for rhizobial infection and nodule organogenesis. However, molecular mechanism through which host legume plants sense NopP remains largely unknown. Here, we constructed an nopP deletion mutant Mesorhizobium huakuii found that negatively regulates on Chinese milk vetch (Astragalus sinicus). Screening interacting proteins in using yeast 2-hybrid system identified 43 (AsNIP43), encodes a...
Abstract Aims To understand the species composition, relative content, and diversity of nitrogen fixing bacteria in Cenchrus fungigraminus rhizophere, to screen nitrogen-fixing study their potential role plant growth promotion. Methods Soil samples were collected from 4 depth (G1, G2, G3 G4) C. rhizophere physical chemical properties determined. The abundance nif H gene copy analyzed. Nitrogen-fixing screened selected for studying seedlings Results highest observed rhizosphere (G2), which...