A5011082347- Organ and Tissue Transplantation Research
- Reconstructive Surgery and Microvascular Techniques
- Reconstructive Facial Surgery Techniques
- Tissue Engineering and Regenerative Medicine
- Xenotransplantation and immune response
- Mesenchymal stem cell research
- Transplantation: Methods and Outcomes
- Urological Disorders and Treatments
- Pluripotent Stem Cells Research
- Digital Imaging in Medicine
- Ear Surgery and Otitis Media
- Urologic and reproductive health conditions
- Diversity and Career in Medicine
- Ocular Disorders and Treatments
- Global Health Workforce Issues
- Genital Health and Disease
- Organ Donation and Transplantation
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Surgical Sutures and Adhesives
- Animal Genetics and Reproduction
- Wound Healing and Treatments
- 3D Printing in Biomedical Research
- Cardiac Valve Diseases and Treatments
- Dental Education, Practice, Research
- Muscle Physiology and Disorders
University of Central Florida
2025
Florida College
2025
Nemours Children's Clinic
2023-2024
Cincinnati Children's Hospital Medical Center
2006-2024
Johns Hopkins Medicine
2012-2023
Johns Hopkins University
2014-2023
Italian Association for Cancer Research
2021-2023
Massachusetts General Hospital
2011-2021
University of Michigan–Ann Arbor
2021
Stanford University
2021
The current standard of care for burns requiring operative treatment consists early burn excision and autologous split-thickness skin grafting. However, in large burns, sufficient donor sites may not be available to achieve total coverage, necessitating temporary coverage with allogeneic human cadaver grafts or synthetic substitutes. A previous study from this laboratory demonstrated that alpha-1,3 galactosyltransferase knockout (GalT-KO) miniature swine enjoyed survival comparable...
Abstract Vascularized composite allotransplantation (VCA) has become a clinical reality, prompting research aimed at improving the risk‐benefit ratio of such transplants. Here, we report our experience with gracilis myocutaneous free flap in Massachusetts General Hospital miniature swine as preclinical VCA model. Fourteen animals underwent transfer comprised muscle and overlying skin, each tissue supplied by independent branches femoral vessels. End‐to‐end anastomoses were performed to...
Two‐dimensional (2D) cell cultures have been extensively used to investigate stem biology, but new insights show that the 2D model may not properly represent potential of tissue origin. Conversely, three‐dimensional exhibit protein expression patterns and intercellular junctions are more representative their in vivo condition. Multiclonal cells grow suspension defined as “spheroids,” we previously demonstrated spheroids from adipose‐derived (S‐ASCs) displayed enhanced regenerative...
Background The recent rise in medical tourism, especially for cosmetic procedures, has been mirrored by an increase the incidence of infections with Mycobacterium abscessus , which is atypical mycobacterium that ubiquitous aquatic environments. M. soft tissue arise from use improperly sterilized water and surgical equipment during these have devastating consequences if not promptly treated. are notoriously difficult to diagnose properly treat, therefore, we illustrate a typical case...
Introduction: Adipose tissue represents an abundant source of mesenchymal stem-like cells. Adipose-derived stem cell progeny have been investigated and used in regenerative medicine for decades. In the last few years, they to “enrich” lipoaspirates fat grafting techniques, attempt boost potential adipose when as autologous “filler”. Materials Methods: 50 samples from subcutaneous breast biopsies were generate floating spheroid (ASphC) lines. ASphC characterized expression putative markers...
Limb salvage in fungal osteomyelitis of the post-traumatic lower extremity represents a difficult clinical problem requiring aggressive management. We report by radical bony debridement, free tissue transfer, distraction osteogenesis with bone-docking, and novel antifungal regimen setting infection Scedosporium inflatum, historically amputation 100% cases. treated inflatum tibia calcaneus debridement infected bone, partial medial rectus abdominis muscle flap coverage, transport osteogenesis,...
Background: The purpose of this study was to assess the efficacy biodegradable, electrospun poly(ε-caprolactone) nanofiber nerve conduits in improving regeneration. Methods: authors used a rat forelimb chronic denervation model effects on regeneration and upper extremity function. Three groups rats were examined: (1) negative-control animals ( n = 5), which underwent 8 weeks median injury followed by repair with no conduit; (2) experimental conduit wrapping coaptation site; (3)...
Abstract Calvarial defects can result from several causes. Tissue engineering hold the potential to restore native form and protective function. We have recently shown that stemness differentiation ability of spheroids adipose-derived stem cells (S-ASCs) promotes osteoblasts growth within Integra in a small vertebral lesion. In our study, we aimed test osteogenic S-ASCs aiding regeneration calvarial defect. Groups containing showed increased bone at defect-Integra interface compared with...
Background: The objective of this meta-analysis was to examine the effectiveness keloid intralesional excision (KILE) in preventing recurrence. Treatment keloids using surgical alone leads high rates To date, there are no widely accepted guidelines for treatment, and a multitude adjunctive therapies used reduce Despite these efforts, recurrence remains high. In study, we conducted existing literature on KILE determine its role reduction. Methods: A review PubMed, Scopus, Web Science...
Reconstruction of limb‐threatening lower extremity defects presents unique challenges. The selected method must provide adequate coverage exposed bone, joints, and tendons while maximizing function the limb. traditional workhorse flaps, free latissimus dorsi rectus abdominis have been associated with donor site morbidity bulkiness that can impair rehabilitation. We report a case series ( n = 18) in which serratus anterior muscle flap split thickness skin graft (STSG) was used for limb soft...
We have previously reported that Massachusetts General Hospital miniature swine, which had accepted class I-mismatched kidneys long-term after 12 days of high-dose cyclosporine A, uniformly donor-major histocompatibility complex (MHC)-matched without immunosuppression but rejected donor MHC-matched split-thickness skin grafts by day 25, changes in renal graft function or antidonor vitro responses. now tested whether this "split tolerance" would also be observed for the primarily vascularized...
Background: Despite encouraging outcomes in vascularized composite allograft (VCA) transplantation, the risks of chronic immunosuppression limit widespread applicability. It has been suggested that infusion donor bone marrow along with VCA may reduce level required to prevent clinical rejection. However, no clear evidence yet presented confirm role prevention In this study we investigated immunologic effects concurrent transplantation a large animal model. Methods: MGH miniature swine (n=4)...
The eyebrow region is of utmost importance for facial movement, symmetry, and the overall cosmetic appearance face. Trauma or tumor resection often leave scars that may dislocate producing an alteration both in static symmetry face dynamic expressivity. authors present a technique eyebrow’s defects repair using remaining advancement by means “freestyle‐like” V‐Y flap. In past two years total eight consecutive patients underwent excision skin lesions superciliary immediate reconstruction with...