A5062318223- Advanced MRI Techniques and Applications
- Radiomics and Machine Learning in Medical Imaging
- Medical Imaging Techniques and Applications
- Cancer Genomics and Diagnostics
- Head and Neck Cancer Studies
- Lung Cancer Diagnosis and Treatment
- Lung Cancer Treatments and Mutations
- Single-cell and spatial transcriptomics
- RNA modifications and cancer
- Extracellular vesicles in disease
- Diet and metabolism studies
- Gut microbiota and health
- Respiratory viral infections research
- Immune responses and vaccinations
- Delphi Technique in Research
- RNA Research and Splicing
- Child Nutrition and Water Access
- Bacteriophages and microbial interactions
- Immune cells in cancer
- Emergency and Acute Care Studies
- Esophageal Cancer Research and Treatment
- IL-33, ST2, and ILC Pathways
- Click Chemistry and Applications
- Epigenetics and DNA Methylation
- Gastrointestinal motility and disorders
Washington University in St. Louis
2012-2024
Medical College of Wisconsin
2024
Milwaukee VA Medical Center
2024
Pathobiont-associated cachexia in a gnotobiotic model of childhood undernutrition is determined by strain-level interactions within the gut microbiota.
Significance Comparing germ-free mice with those colonized at birth or later provides a way to determine how gut microbial community exposure affects the chromatin landscape of cells along remote sites, ascertain alterations in accessibility are correlated functional features different lineages, and whether there is critical window when signals must be received alter durably. Genome-wide analysis intraepithelial lymphocytes circulating T purified from gnotobiotic revealed enhancers flanking...
Epigenetic regulation plays a crucial role in the pathogenesis of autoimmune diseases such as inflammatory arthritis. DNA hypomethylating agents, decitabine (DAC), have been shown to dampen inflammation and restore immune homeostasis. In present study, we demonstrate that DAC elicits potent anti-inflammatory effects attenuates disease symptoms several animal models Transcriptomic epigenomic profiling show DAC-mediated hypomethylation regulates wide range cell types arthritis, altering...
Background: Multicellular programs in the tumor microenvironment (TME) drive cancer pathogenesis and response to therapy but remain challenging identify profile clinically. Here, we present Spatial EcoTyper, a multimodal machine learning framework for large-scale profiling of spatially dependent cell states multicellular ecosystems, termed spatial ecotypes (SEs). Methods Findings: By integrating five million single-cell spot-level transcriptomes from 10 diverse human neoplasms, including...
Abstract The optimal treatment paradigm for patients with oligometastatic non-small cell lung cancer (NSCLC) remains unclear. Some disease experience prolonged remission after locally consolidative radiation therapy (RT), while others harbor micrometastatic (below limits of detection by imaging) and benefit from systemic therapy. To risk-stratify identify the most likely to RT, we performed a multi-institutional cohort study 1487 NSCLC undergoing liquid biopsy analysis circulating tumor DNA...
Abstract Purpose: Our goal was to demonstrate that lymphatic drainage fluid (lymph) has improved sensitivity in quantifying postoperative minimal residual disease (MRD) locally advanced human papillomavirus (HPV)-associated oropharyngeal squamous cell carcinoma (OPSCC) compared with plasma, and leverage this novel biofluid for patient risk stratification. Experimental Design: We prospectively collected lymph samples from neck drains of 106 patients HPV (+) OPSCC, along 67 matched plasma...
<p>Characterization of lymphatic cfDNA and development a liquid biopsy assay</p>
<p>HPV (+) OPSCC recurrences stratified by postoperative lymph cf-HPV detection, plasma or high-risk pathology.</p>
<p>Blood contamination does not significantly impact lymph cf-HPV measurement.</p>
<p>HPV (+) OPSCC recurrences stratified by postoperative lymph cf-HPV detection, plasma or high-risk pathology.</p>
<div>AbstractPurpose:<p>Our goal was to demonstrate that lymphatic drainage fluid (lymph) has improved sensitivity in quantifying postoperative minimal residual disease (MRD) locally advanced human papillomavirus (HPV)-associated oropharyngeal squamous cell carcinoma (OPSCC) compared with plasma, and leverage this novel biofluid for patient risk stratification.</p>Experimental Design:<p>We prospectively collected lymph samples from neck drains of 106 patients HPV (+)...
<p>Blood contamination does not significantly impact lymph cf-HPV measurement.</p>
<p>Cell-free HPV is enriched in the lymph relative to patient-matched plasma and does not correlate with primary tumor features or smoking history</p>
<div>AbstractPurpose:<p>Our goal was to demonstrate that lymphatic drainage fluid (lymph) has improved sensitivity in quantifying postoperative minimal residual disease (MRD) locally advanced human papillomavirus (HPV)-associated oropharyngeal squamous cell carcinoma (OPSCC) compared with plasma, and leverage this novel biofluid for patient risk stratification.</p>Experimental Design:<p>We prospectively collected lymph samples from neck drains of 106 patients HPV (+)...
<p>Designing a machine learning model that integrates postoperative lymph and plasma MRD to stratify outcomes</p>
<p>Designing a machine learning model that integrates postoperative lymph and plasma MRD to stratify outcomes</p>
<p>Supplementary methods found here.</p>