A5021982433- Immune Response and Inflammation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Antibiotic Resistance in Bacteria
- Immune cells in cancer
- Vibrio bacteria research studies
- Study of Mite Species
- Immunotherapy and Immune Responses
- Dermatological diseases and infestations
- Immune Cell Function and Interaction
- Mast cells and histamine
- Bacterial biofilms and quorum sensing
- Bird parasitology and diseases
- Neonatal and Maternal Infections
- Identification and Quantification in Food
- Environmental DNA in Biodiversity Studies
- Forensic and Genetic Research
- Cultural Heritage Materials Analysis
- Molecular Biology Techniques and Applications
- Escherichia coli research studies
- Chemokine receptors and signaling
- Toxoplasma gondii Research Studies
- Parasitic Infections and Diagnostics
- Antimicrobial Peptides and Activities
- Enterobacteriaceae and Cronobacter Research
- Antibiotic Use and Resistance
Teikyo University
2013-2023
Osaka University
2020-2022
Jikei University School of Medicine
2020
Tokyo Metropolitan Institute of Public Health
2020
Kagoshima University
2020
Acinetobacter baumannii causes nosocomial infections due to its multidrug resistance and high environmental adaptability. Colistin is a polypeptide antibacterial agent that targets lipopolysaccharide (LPS) currently used control serious multidrug-resistant Gram-negative bacterial infections, including those caused by A. baumannii. However, may acquire colistin losing their LPS. In mouse models, LPS-deficient have attenuated virulence. Nevertheless, the mechanism through which pathogen...
Hospital-acquired infections caused by Acinetobacter baumannii have become problematic because of high rates drug resistance. A. is usually harmless, but it may cause infectious diseases in an immunocompromised host. Although neutrophils are the key players initial immune response against bacterial infection, their interactions with remain largely unknown. A new biological defense mechanism, termed neutrophil extracellular traps (NETs), has been attracting attention. NETs play a critical...
Neutrophils play a critical role in the innate immune response. Recently, new neutrophilic biological defense mechanism, termed neutrophil extracellular traps (NETs), has been attracting attention. have observed to release both lysosomal enzymes and their nuclear contents, including unfolded chromatin, which together trap inactivate bacteria. The environment tissues where neutrophils act is thought be different from that of blood serum. In this study, we assessed effect serum on NET...
Abstract Hospital-acquired infections as a result of Acinetobacter baumannii have become problematic because high rates drug resistance. Although neutrophils play critical role in early protection against bacterial infection, their interactions with A. remain largely unknown. To elucidate the between and human neutrophils, we cocultured these cells analyzed them by microscopy flow cytometry. We found that adhered to neutrophils. next examined neutrophil infiltration into Matrigel basement...
Colistin is used to treat infectious diseases in humans and livestock; it has also been as a feed additive for livestock approximately 50 years. Since the mcr-1 plasmid-mediated colistin resistance gene was discovered China 2015, detected worldwide, mainly livestock. In this study, we investigated prevalence characteristics of mcr -mediated colistin-resistant Escherichia coli farmers Japan. We collected fecal samples from 295 healthy (202 cattle 93 swine) 62 72 farms (58 14 swine farms)...
Radioprotective 105 (RP105) is a type I transmembrane protein, which associates with glycoprotein, MD-1. Monoclonal antibody (mAb)-mediated ligation of RP105/MD-1 robustly activates B cells. structurally similar to Toll-like receptor 4 (TLR4)/MD-2. B-cell responses TLR2 and TLR4/MD-2 ligands are impaired in the absence RP105 or In addition RP105/MD-1, MD-1 alone secreted. The structure shows that has hydrophobic cavity directly binds phospholipids. Little known, however, about ligand for...
We investigated the effects of two antibiotics, erythromycin and rifampicin, on immunomodulatory gene expression cellular function human polymorphonuclear leukocytes (PMNs).We used real-time quantitative PCR to examine genes. The production reactive oxygen species (ROS) was determined by fluorescence-activated cell sorting. PMN chemotaxis analyzed using a KK chamber.Stimulation PMNs with lipopolysaccharide (LPS) resulted in increases mRNA levels Rifampicin significantly inhibited...
Abstract Scabies is a highly contagious skin disease caused by the mite Sarcoptes scabiei that affects many mammals. However, sensitivity of traditional tests for scabies diagnosis in humans less than 50%. To simplify scabies, methods are simple, sensitive, specific, and cost-effective required. We developed an immunodiagnostic test based on S. var. nyctereutis RNA-seq data collected from Japanese raccoon dogs with sarcoptic mange. Three candidate antigens—a expressed hypothetical protein...
Acinetobacter baumannii is the main causative pathogen of nosocomial infections that causes severe in lungs. In this study, we analyzed histopathological characteristics lung infection with two strains A. (ATCC 19606 and clinical isolate TK1090) Pseudomonas aeruginosa PAO-1 C3H/HeN mice to evaluate virulence baumannii. Survival was evaluated over 14 days. At 1, 2, 5, or days postinfection, were sacrificed, analysis specimens also performed. Histopathological changes accumulation neutrophils...
Herein, we investigated the effect of bacterial lipooligosaccharides (LOS), from Acinetobacter baumannii, on expression pro-inflammatory genes that play an essential role in clearance. LAD2 human mast cells were stimulated with LOS derived two strains A. baumannii—ATCC 19606 and MDRA T14. exposure induced for mediators, including TNF-α, IL-8, LTC4S, CCL4, TLR4. The mRNA levels a majority genes, except TLR4, baumannii-LOS increased. Moreover, co-culture neutrophils supernatant obtained (ATCC...
Abstract Gelatin, sourced from collagen, is an acid-, alkali- or enzymatically hydrolysed product obtained animal skins and bones. Gelatin has been widely used for the manufacture of various cultural objects, e.g. as a water-soluble binder dissolving pigments, glue musical instruments traditional crafts along with human history. The identification species in gelatin, hence, could provide critical clue understanding history including lifestyles, culture technologies. However, there no valid...
Recent advances in molecular biology have greatly contributed to archaeological research, thus propagating drastic changes methodology.Especially, developments nucleic acid sequence analysis together with resulting genome database much facilitated identification of bioorganisms, for example, originated from bones [1-2], sediments [3][4], pollen [5], charred grains [6], and parasite eggs [7].However, points view, experimentally-accessible samples suitable further sequential are typically very...