A5100652864- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Carbohydrate Chemistry and Synthesis
- Enzyme Production and Characterization
- Synthesis and Catalytic Reactions
- Tea Polyphenols and Effects
- Carbon and Quantum Dots Applications
- Quinazolinone synthesis and applications
- Medical Imaging Techniques and Applications
- Peptidase Inhibition and Analysis
- Nanocluster Synthesis and Applications
- Sulfur-Based Synthesis Techniques
- Enzyme Catalysis and Immobilization
- Proteoglycans and glycosaminoglycans research
- Lipid metabolism and biosynthesis
- Glycosylation and Glycoproteins Research
- Lysosomal Storage Disorders Research
- Protein Degradation and Inhibitors
- Cancer Mechanisms and Therapy
- Synthesis and Characterization of Heterocyclic Compounds
- Radiopharmaceutical Chemistry and Applications
- Synthesis and Biological Evaluation
- Lanthanide and Transition Metal Complexes
- Oral microbiology and periodontitis research
- Phenothiazines and Benzothiazines Synthesis and Activities
Leiden University
2018-2024
Beijing Normal University
2010-2013
Amylases are key enzymes in the processing of starch many kingdoms life. They important catalysts industrial biotechnology where they applied in, among others, food and production detergents. In man amylases first digestion to glucose arguably also preferred target therapeutic strategies aimed at treatment type 2 diabetes patients through down-tuning assimilation. Efficient sensitive assays that report selectively on retaining amylase activities irrespective nature complexity biomaterial...
Cyclophellitol aziridine and its configurational functional isomers are powerful covalent inhibitors of retaining glycosidases, find application in fundamental studies on amongst others relation to inherited lysosomal storage disorders caused by glycosidase malfunctioning. Few direct stereoselective aziridination methodologies known for the synthesis cyclophellitol aziridines. Herein, we present our scope 3-amino-2-(trifluoromethyl)quinazolin-4(3H)-one-mediated a variety cyclohexenol...
Gaucher disease (GD) is a lysosomal storage disorder caused by inherited deficiencies in β-glucocerebrosidase (GBA). Current treatments require rapid diagnosis and means of monitoring therapeutic efficacy, both which may be supported the use GBA-targeting activity-based probes (ABPs). Here, we report synthesis structural analysis range cyclophellitol epoxide aziridine inhibitors ABPs for GBA. We demonstrate their covalent mechanism-based mode action uncover binding new N-functionalised...
α‐Amylases are the workhorse enzymes of starch degradation. They central to human health, including as targets for anti‐diabetic compounds, but also key in industrial processing biofuels, corn syrups, brewing and detergents. Dissection activity, specificity stability α‐amylases is crucial understanding their biology allowing exploitation. Yet, functional characterization lags behind DNA sequencing genomics; new tools required rapid analysis α‐amylase function. Here, we design, synthesize...
α‐Amylases are the workhorse enzymes of starch degradation. They central to human health, including as targets for anti‐diabetic compounds, but also key in industrial processing biofuels, corn syrups, brewing and detergents. Dissection activity, specificity stability α‐amylases is crucial understanding their biology allowing exploitation. Yet, functional characterization lags behind DNA sequencing genomics; new tools required rapid analysis α‐amylase function. Here, we design, synthesize...
Siastatin B is a potent and effective iminosugar inhibitor of three diverse glycosidase classes, namely, sialidases, β-d-glucuronidases, N-acetyl-glucosaminidases. The mode inhibition glucuronidases, in contrast to has long been enigmatic as siastatin appears too bulky incorrectly substituted be accommodated within β-d-glucuronidase active site pocket. Herein, we show through crystallographic analysis protein-inhibitor complexes that generates both hemiaminal 3-geminal diol (3-GDI) are,...
Twenty-six novel 1,2,4-triazolo [3,4-b][1,3,4] thiadiazines containing a furan and thiophene moiety are synthesized evaluated for their biological activities.
Fermentation is key to the quality of black tea, and traditional sensory evaluation methods are too slow subjective for reliable, large-scale during fermentation. To address this problem, study formulated a sensing system based on cobalt ions-doped carbon quantum dots emitting bright blue fluorescence. In experiments, was found work stably 90 min accurately determine fermentation in real time. fluorescence spectra results, intensity decreased sharply with concentrations catechins,...