A5103250416- Fatty Acid Research and Health
- Eicosanoids and Hypertension Pharmacology
- Inflammatory mediators and NSAID effects
- Pain Mechanisms and Treatments
- Viral Infectious Diseases and Gene Expression in Insects
- Peroxisome Proliferator-Activated Receptors
- Calpain Protease Function and Regulation
- Mitochondrial Function and Pathology
- Animal Virus Infections Studies
- Cancer, Lipids, and Metabolism
- Virus-based gene therapy research
- Adipose Tissue and Metabolism
- Neurological Disorders and Treatments
- Biochemical effects in animals
- Diet and metabolism studies
- Plant Pathogens and Fungal Diseases
- Estrogen and related hormone effects
- Fibroblast Growth Factor Research
- Soil Moisture and Remote Sensing
- Plant Virus Research Studies
- Neurological diseases and metabolism
- Metabolism and Genetic Disorders
- Parkinson's Disease Mechanisms and Treatments
- Seaweed-derived Bioactive Compounds
- Nutrition, Genetics, and Disease
Eastern Liaoning University
2017-2024
Huazhong University of Science and Technology
2023
Tongji Hospital
2023
Second Affiliated Hospital of Fujian Medical University
2023
Fujian Medical University
2023
University of Michigan
2007-2016
Michigan United
2013
Taipei Municipal Jen-Ai Hospital
2010
Second Affiliated Hospital of Xi'an Jiaotong University
2009
Nu Skin (United States)
2007
Dietary fish oil containing ω3 highly unsaturated fatty acids has cardioprotective and anti-inflammatory effects. Prostaglandins (PGs) thromboxanes are produced in vivo both from the ω6 acid arachidonic (AA) eicosapentaenoic (EPA). Certain beneficial effects of may result altered PG metabolism resulting increases EPA/AA ratios precursor phospholipids. Here we report vitro specificities prostanoid enzymes receptors toward EPA-derived, 3-series versus AA-derived, 2-series substrates products....
Dynamin-related protein (Drp) 1 is a key regulator of mitochondrial fission and composed GTP-binding, Middle, insert B, C-terminal GTPase effector (GED) domains. Drp1 associates with sites promotes membrane constriction through its intrinsic activity. The mechanisms that regulate activity remain poorly understood but are likely to involve reversible post-translational modifications, such as conjugation small ubiquitin-like modifier (SUMO) proteins. Through detailed analysis, we find...
Diabetic neuropathy is a common complication of diabetes. While multiple pathways are implicated in the pathophysiology diabetic neuropathy, there no specific treatments and means to predict onset or progression. Here, we identify gene expression signatures related develop computational classification models Microarray experiments were performed on 50 samples human sural nerves collected during 52-week clinical trial. A series bioinformatics analyses identified differentially expressed genes...
OBJECTIVE A better understanding of the molecular mechanisms underlying development and progression diabetic neuropathy (DN) is essential for design mechanism-based therapies. We examined changes in global gene expression to define pathways regulated by diabetes peripheral nerve. RESEARCH DESIGN AND METHODS Microarray data 24-week-old BKS db/db db/+ mouse sciatic nerve were analyzed significantly differentially expressed genes (DEGs); DEGs further identify biological processes pathways....
C57BLKS db/db (db/db) mice develop a neuropathy with features of human type 2 diabetic neuropathy. Here, we demonstrate that these transient mechanical allodynia at the early stage diabetes. We hypothesized nerve growth factor (NGF), which enhances expression key mediators nociception (i.e. substance P [SP] and calcitonin gene-related peptide), contributes to development in mice. found NGF, SP, peptide gene is upregulated dorsal root ganglion (DRG) before or during period they allodynia....
Recombinant human prostaglandin endoperoxide H synthase-1 (huPGHS-1) was characterized. huPGHS-1 has a single high-affinity heme binding site per dimer and exhibits maximal cyclooxygenase (COX) activity with one dimer. Thus, functions as conformational heterodimer having catalytic monomer (Ecat) bound an allosteric (Eallo) lacking heme. The enzyme is modestly inhibited by common FAs including palmitic, stearic, oleic acids that are not COX substrates. Studies of arachidonic acid (AA)...
Background: Painful Diabetic Neuropathy (PDN) affects more than 25% of patients with type 2 diabetes; however, the pathogenesis remains unclear due to lack knowledge molecular mechanisms leading PDN. In our current study, we use an animal model diabetes in order understand roles p38 Previously, have demonstrated that C57BLK db/db (db/db) mouse, a carries loss-of-function leptin receptor mutant, develops mechanical allodynia hind paws during early stage (6–12 wk age) diabetes. Using this...
High circulating long chain fatty acids (LCFAs) are implicated in diabetic neuropathy (DN) development. Expression of the long-chain acyl-CoA synthetase 1 (Acsl1) gene, a gene required for LCFA metabolic activation, is altered human and mouse peripheral nerve. We assessed significance Acsl1 upregulation primary cultured Schwann cells.
Abstract Background Reactive oxygen species (ROS) are known mediators of cellular damage in multiple diseases including diabetic complications. Despite its importance, no comprehensive database is currently available for the genes associated with ROS. Methods We present ROS- and diabetes-related targets (genes/proteins) collected from biomedical literature through a text mining technology. A web-based tool, SciMiner, was applied to 1,154 papers indexed diabetes ROS by PubMed identify...
Abstract In amyotrophic lateral sclerosis caused by mutations in Cu/Zn‐superoxide dismutase (SOD1), altered solubility and aggregation of the mutant protein implicates failure pathways for detecting catabolizing misfolded proteins. Our previous studies demonstrated early reduction proteasome‐mediated proteolytic activity lumbar spinal cord SOD1 G93A transgenic mice, tissue particularly vulnerable to disease. The purpose this study was identify any underlying abnormalities proteasomal...
Abstract The balance of putative pro- and antiinflammatory lipoxygenase (LOX)-derived S-hydroxyeicosatetraenoic acids (S-HETEs) in colon mucosa is a potential target for modulating cancer risk progression. biological effects S-HETEs R-hydroxyeicosatetraenoic (produced by distinct pathways) may differ, but levels these compounds the are unknown. objective this study was to develop chiral methods characterize hydroxyeicosatetraenoic (HETE) enantiomers colonic evaluate fish oil on HETE...
Most stem cell therapies involve direct, intraparachymal placement of neural progenitor cells. These cells provide physical support to the endogenous neuronal population and may be engineered in situ growth factor support. Insulin-like factor-I (IGF-I) has potent neurotrophic neuroprotective properties is expressed by human (hNSCs). IGF-I implicated multiple aspects behavior, including proliferation, differentiation, survival. Enhancing hNSC function through overexpression increase benefits...
Dynamin-2 (DNM2) is a large GTPase involved in clathrin-mediated endocytosis and related trafficking pathways. Mutations human DNM2 cause two distinct neuromuscular disorders: centronuclear myopathy Charcot-Marie-Tooth disease. Zebrafish have been shown to be an excellent animal model for many neurologic disorders, this system has the potential inform our understanding of DNM2-related Currently, little known about endogenous zebrafish orthologs DNM2. In study, we characterize dynamin-2...
Prostaglandin E2 (PGE2) has been linked to a higher risk of colorectal cancer. PGE2 in colon tissue can be reduced by increasing dietary eicosapentaenoic acid (EPA). The dose-dependent relationships between EPA, serum EPA:arachidonate (AA) ratio, urinary metabolites, and colonic eicosanoids were evaluated develop biomarkers for prediction PGE2. Male rats fed diets containing EPA:ω6 fatty ratios 0, 0.1, 0.2, 0.4, or 0.6 5 weeks. Increasing the ratio increased EPA:AA proximal, transverse,...
Prostaglandin (PG) endoperoxide H synthase (PGHS)-2, also known as cyclooxygenase (COX)-2, can convert arachidonic acid (AA) to PGH2 in the committed step of PG synthesis. PGHS-2 functions a conformational heterodimer composed an allosteric (Eallo) and catalytic (Ecat) monomer. Here we investigated interplay between human (hu)PGHS-2 alternative COX substrate, endocannabinoid, 2-arachidonoylglycerol (2-AG), well stable analog, 2-O-arachidonylglycerol ether (2-AG ether). We compared inhibition...
Western diets are enriched in omega-6 vs. omega-3 fatty acids, and a shift this balance toward acids may have health benefits. There is limited information about the catabolism of 3-series prostaglandins (PG) formed from eicosapentaenoic acid (EPA), fish oil that becomes elevated tissues following consumption. Quantification appropriate urinary PG metabolites could be used for noninvasive measurement tone. Here we describe preparation tritium- deuterium-labeled 6-keto-PGF2α their use...
Deep sea water (DSW) is a natural resource rich in minerals, which participates biological processes such as energy metabolism, regulates serum glucose and lipids levels, has certain protective effect on endocrine metabolism-related diseases. Studies have shown that the improvement of tolerance diabetic mice by DSW may be associated with structure function pancreatic islets, specific mechanism still unclear. Other studies long-term exposure to high concentrations fatty acids can lead...
Abstract Purpose This study aims to explore the role of GATA6 in lung cancer, with a focus on its impact metabolic processes. Methods We assessed expression cancer tissues and association patient prognosis. In vitro cell function experiments were conducted investigate effects altered levels proliferation migration. Mechanistic insights gained by examining GATA6's influence glucose metabolism-related genes, particularly effect c-Myc mRNA expression. Results Our revealed significant...