A5022058493- Immunotherapy and Immune Responses
- RNA Interference and Gene Delivery
- Extracellular vesicles in disease
- 3D Printing in Biomedical Research
- Cancer Cells and Metastasis
- Cancer Genomics and Diagnostics
- Innovative Microfluidic and Catalytic Techniques Innovation
- SARS-CoV-2 and COVID-19 Research
- MicroRNA in disease regulation
- Immune cells in cancer
- Circular RNAs in diseases
- Electrowetting and Microfluidic Technologies
- Peroxisome Proliferator-Activated Receptors
- COVID-19 Clinical Research Studies
- Electrohydrodynamics and Fluid Dynamics
- Microfluidic and Bio-sensing Technologies
- Cancer-related molecular mechanisms research
- Genomics, phytochemicals, and oxidative stress
- SARS-CoV-2 detection and testing
- Reproductive System and Pregnancy
- Surface Modification and Superhydrophobicity
- vaccines and immunoinformatics approaches
- Biosensors and Analytical Detection
- Peptidase Inhibition and Analysis
Wuhan University
2020-2024
Zhongnan Hospital of Wuhan University
2020-2024
Abstract Background Dendritic cells (DCs) are central for the initiation and regulation of innate adaptive immunity in tumor microenvironment. As such, many kinds DC-targeted vaccines have been developed to improve cancer immunotherapy numerous clinical trials. Targeted delivery antigens adjuvants DCs vivo represents an important approach development DC vaccines. However, nonspecific activation systemic preparation optimal immunodominant still represent major challenges. Methods We loaded...
Abstract Current organoid models are limited by the incapability of rapidly fabricating organoids that can mimic immune microenvironment for a short term. Here, an acoustic droplet‐based platform is presented to facilitate rapid formation tumor organoids, which retains original and establishes personalized bladder cancer immunotherapy model. In combination with hydrophobic substrate, droplet printer yield large number homogeneous highly viable in vitro within week. The generated consist all...
As carriers of biomolecules (proteins, nucleic acids, and lipids) from parent cells, exosomes play a significant role in physiology pathology. In any diseased state, the morphology released remained similar. The contents change depending on disease or its stage; thus, are generally considered as "source biomarkers". Therefore, they promising biomarkers for diagnosis prognosis tumors. natural delivery vehicles, can protect their cargo immune clearance deliver them to other cells through...
Cancer-associated fibroblasts (CAFs) are the key components of immune barrier in liver cancer. Therefore, gaining a deeper understanding heterogeneity and intercellular communication CAFs holds utmost importance boosting immunotherapy effectiveness improving clinical outcomes.
Sulforaphane (SFN) is a compound derived from cruciferous plants shown to be effective in cancer prevention and suppression. Myeloid-derived suppressor cells (MDSCs) are known inhibit anti-tumor immunity; however, whether SFN regulates the activity of MDSCs breast still unknown. In current study, we found that blocked prostaglandin E2 (PGE2) synthesis parental doxorubicin (DOX)-resistant 4T1 cell lines by activating NF-E2-related factor 2 (Nrf2). Nrf2-mediated reduction PGE2 was dependent on...
Liquid droplets rectors have been used in clinical diagnosis, high throughput screening and bioassay. However, it is challenging for droplet reactors to be practical applications due the difficulty of uniformly mixing ultrasmall volumes samples lack rapid high-precision detection protocols. Here, we developed an acoustic system efficient biological chemical screening. By employing wave devices, nondestructive uniform ∼nL−μL can achieved. acoustophoretic force, perturbation quickly...
Abstract Patient personalized treatment models can predict response and assist in determining the optimal option for individual patients. The tumor microenvironment mediates development prognosis. However, current such as patient‐derived xenograft (PDX) 2D culture fail to preserve microenvironment. Herein, a technique acoustic‐droplet‐printing‐of‐patient‐derived‐organoids (ADPDOs) is reported, which allows high‐throughput, high‐viability, rapid, uniform printing preparation of organoids. In...
Cryopreservation is a key step for current translational medicine including reproductive medicine, regenerative and cell therapy. However, it challenging to preserve rare cells practical applications due the difficulty in handling low numbers of as well lack highly efficient biocompatible preservation protocols. Here, we developed an acoustic droplet vitrification method high-efficiency cells. By employing ejection device, can encapsulate into water-in-air droplets with volume from ∼pL ∼nL...