A5037305276- HIV Research and Treatment
- Spinal Fractures and Fixation Techniques
- Hip and Femur Fractures
- Advanced Fluorescence Microscopy Techniques
- Signaling Pathways in Disease
- Blood groups and transfusion
- Pelvic and Acetabular Injuries
- Bone Tumor Diagnosis and Treatments
- Spine and Intervertebral Disc Pathology
- Genomics and Chromatin Dynamics
- Bacterial Genetics and Biotechnology
- Sarcoma Diagnosis and Treatment
- DNA Repair Mechanisms
- RNA Research and Splicing
- Autophagy in Disease and Therapy
- MicroRNA in disease regulation
- Bone fractures and treatments
- Genomics, phytochemicals, and oxidative stress
- Catalysis and Oxidation Reactions
- Zeolite Catalysis and Synthesis
- Extracellular vesicles in disease
- Sirtuins and Resveratrol in Medicine
- Bacteriophages and microbial interactions
- Digital Holography and Microscopy
- Inflammatory Biomarkers in Disease Prognosis
Sichuan University
2018-2025
West China Hospital of Sichuan University
2018-2025
University of Pittsburgh
2003-2023
UPMC Hillman Cancer Center
2022-2023
Huazhong University of Science and Technology
2021
Central Hospital of Wuhan
2011-2021
Chengdu University
2018
Chongqing Medical University
2018
University of Wisconsin–Madison
2015
The Central Hospital of Shaoyang
2010
Abstract Single-molecule characterization of protein–DNA dynamics provides unprecedented mechanistic details about numerous nuclear processes. Here, we describe a new method that rapidly generates single-molecule information with fluorescently tagged proteins isolated from extracts human cells. We demonstrated the wide applicability this novel technique on undamaged DNA and three forms damage using seven native repair two structural variants, including: poly(ADP-ribose) polymerase (PARP1),...
HIV is the causative agent of AIDS, which has no cure. The protein shell that encases viral genome, capsid, critical for replication in cells at multiple steps.
Osteoporosis is a common systemic metabolic disease, leading to increased bone fragility and risk of fractures. Research has shown that Adenosine triphosphate (ATP) synthase, H+transporting, mitochondrial F1 complex, alpha subunit 1 (ATP5A1), crucial component in ATP production, inhibited dexamethasone (DEX)-induced osteoblasts. Therefore, this study aimed investigate the molecular mechanism underlying inhibitory impact DEX on osteogenic differentiation rat marrow mesenchymal stem cells...
ABSTRACT Cleavage and polyadenylation specificity factor 6 (CPSF6) is a human protein that binds HIV-1 capsid mediates nuclear transport integration targeting of preintegration complexes. Truncation the at its C-terminal nuclear-targeting arginine/serine-rich (RS) domain produces protein, CPSF6-358, potently inhibits infection by inhibiting entry. To understand molecular mechanism behind this restriction, interaction between CPSF6-358 was characterized using in vitro vivo assays. Purified...
Mesenchymal stem cells (MSCs) are a promising regenerative medicine. The roles of miRNAs in osteogenic differentiation bone marrow MSCs (BM-MSCs) remained less reported. Forkhead Box O3 (FOXO3) and alkaline phosphatase (ALP) levels the BM-MSCs were measured on 3, 7, 14 days after differentiation. After transfection FOXO3 overexpression plasmids or siFOXO3 into BM-MSCs, factors related to cell autophagy determined. Besides, 3-methyladenine rapamycin, as well miR-223-3p mimic inhibitor applied...
Abstract Correlative light and electron microscopy (CLEM) combines the strengths of both imaging modalities enables linking biological spatiotemporal information from live-cell fluorescence (fLM) to high-resolution cellular ultra-structures cryo-electron tomography (cryoEM/ET). This has been previously achieved by using fLM signals localize regions interest under cryogenic conditions. The correlation process, however, is often tedious time-consuming with low throughput limited accuracy,...
UV-DDB is a DNA damage recognition protein recently discovered to participate in the removal of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxoG) by stimulating multiple steps base excision repair (BER). In this study, we examined whether has wider role BER besides oxidized bases and found it specificity for two known substrates alkyladenine glycosylase (AAG)/N-methylpurine (MPG): 1, N6-ethenoadenine (ϵA) hypoxanthine. Gel mobility shift assays show that recognizes these lesions 4-5 times better...
Bacterial cell division involves the dynamic assembly of proteins and coordinated constriction envelope. A wide range factors regulates division--including growth environmental stresses--and targeting machinery has been a widely discussed approach for antimicrobial therapies. This paper introduces divin, small molecule inhibitor bacterial that may facilitate mechanistic studies this process. Divin disrupts late proteins, reduces peptidoglycan remodeling at site, blocks compartmentalization...
Our study aimed to investigate the relationship between MALAT-1 (metastasis-associated lung adenocarcinoma transcript 1) expression and chemotherapy drug resistance in osteosarcoma.The U-2OS osteosarcoma cell line was selected for experiment. The cells were treated with methotrexate, doxorubicin, cisplatin, ifosfamide, respectively. RT-PCR applied detect cells. doxorubicin-resistant constructed. divided into doxorubicin-sensitivity group (DS/shCtrl), doxorubicin-resistance (DR/shCtrl)...
MicroRNA (miR)-21 has been found to be overexpressed in osteosarcoma (OS). The aim of the present study was investigate effect miR-21-5p on Warburg and stemness maintenance OS cells its potential molecular mechanism. Herein, or inhibited MG-63 via transfection with mimics inhibitors. cell viability, apoptosis, were explored cells. results demonstrated that inhibition suppressed viability enhanced their apoptosis. Additionally, attenuated cells, as by reduced proportion CD133-positive...
Multidrug resistance (MDR) due to overexpression of MDR1 is a major obstacle that hinders the treatment hepatocellular carcinoma (HCC). In this study, we explored function and underlying molecular mechanism SIRT6 in multidrug HCC. Chemotherapeutic agents (doxorubicin, cisplatin sorafenib) increased mRNA protein level two HCC cell lines dose-dependent manner. depletion resulted decreased viability apoptosis cells treated with chemotherapeutic agents. Mechanistically, reduced transcription by...
Herein, we describe a convenient approach for the preparation of polymeric micelle using doxorubicin (DOX) conjugated trimethyl-chitosan (TMC) with Beclin-1 siRNA (Si-Beclin1/DOX-TMC). This displayed potent capacity autophagy inhibition and reversed drug-resistance to DOX in BIU-87/ADR cell lines. The Si-Beclin1/DOX-TMC was highly cytotoxic both drug-sensitive BIU-87 drug-resistant cells. Its reverse dependent upon upregulation levels TMC via pH-sensitive Schiff base, which responded acidic...
Motile bacteria navigate chemical environments by using chemoreceptors. The output of these protein sensors is linked to motility machinery and enables follow gradients. Understanding the specificity different families chemoreceptors essential for predicting controlling bacterial behavior in ecological niches, including symbiotic pathogenic interactions with plants mammals. identification chemical(s) recognized specific receptors limited low throughput complexity chemotaxis assays. To...
Abstract Single-molecule characterization of protein-DNA dynamics provides unprecedented mechanistic details about numerous nuclear processes. Here, we describe a new method that rapidly generates single-molecule information for fluorescently tagged proteins isolated from extracts human cells. This approach determines binding lifetimes ( k off ), events per second (∼ on positional dependence (specificity), and characterizes 1D diffusion along DNA. We demonstrated the wide applicability this...
Summary Human immunodeficiency virus type 1 (HIV-1) capsid binds host proteins during infection, including cleavage and polyadenylation specificity factor 6 (CPSF6) cyclophilin A (CypA). We observe that HIV-1 infection induces higher-order CPSF6 formation capsid-CPSF6 complexes co-traffic on microtubules. CPSF6-capsid complex trafficking is impacted by alterations reduce binding or excess cytoplasmic expression, both of which are associated with decreased infection. Higher-order bind disrupt...
Objective To explore the method and evaluate effect of external fixator associated with combined Kirschner wire technique for treatment instable intra-articular distal radial fractures.Methods Forty-six patients fractures were treated technique.According to AO/ASIF classification,there 27 cases type C1 fractures,16 C2 3 C3 fractures.The radiographic parameters evaluated according modified Sarmiento scale regarding volar tilt,radial inclination,radial shortening stepping articular...