A5080905508- Chromosomal and Genetic Variations
- DNA Repair Mechanisms
- Photosynthetic Processes and Mechanisms
- Genomics and Chromatin Dynamics
- CRISPR and Genetic Engineering
- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Plant tissue culture and regeneration
- Microtubule and mitosis dynamics
- Carcinogens and Genotoxicity Assessment
- RNA Research and Splicing
- Genomics and Phylogenetic Studies
- Heat shock proteins research
University of Cambridge
2019-2024
Rothamsted Research
2024
A closer look at centromeres Centromeres are key for anchoring chromosomes to the mitotic spindle, but they have been difficult sequence because can contain many repeating DNA elements. These repeats, however, carry regularly spaced, distinctive markers of heterogeneity between mostly, not completely, identical repeats. Such differences aid assembly. Naish et al . used ultra-long-read sequencing establish a reference assembly that resolves all five in small mustard plant Arabidopsis Their...
During meiosis, interhomolog recombination produces crossovers and noncrossovers to create genetic diversity. Meiotic frequency varies at multiple scales, with high subtelomeric suppressed centromeric typical in many eukaryotes. recombination, sister chromatids are tethered as loops a polymerized chromosome axis, which, plants, includes the ASY1 HORMA domain protein REC8–cohesin complexes. Using chromatin immunoprecipitation, we show an ascending telomere-to-centromere gradient of...
Meiosis recombines genetic variation and influences eukaryote genome evolution. During meiosis, DNA double-strand breaks (DSBs) enter interhomolog repair to yield crossovers noncrossovers. DSB occurs as replicated sister chromatids are connected a polymerized axis. Cohesin rings containing the REC8 kleisin subunit bind anchor chromosomes Here, we report genomic landscape of using chromatin immunoprecipitation sequencing (ChIP-seq) in Arabidopsis (Arabidopsis thaliana). associates with...
Abstract Centromeres attach chromosomes to spindle microtubules during cell division and, despite this conserved role, show paradoxically rapid evolution and are typified by complex repeats. We used ultra-long-read sequencing generate the Col-CEN Arabidopsis thaliana genome assembly that resolves all five centromeres. The centromeres consist of megabase-scale tandemly repeated satellite arrays, which support high CENH3 occupancy densely DNA methylated, with variants private each chromosome....
During meiosis, DNA double-strand breaks (DSBs) occur throughout the genome, a subset of which are repaired to form reciprocal crossovers between chromosomes. Crossovers essential ensure balanced chromosome segregation and create new combinations genetic variation. Meiotic DSBs formed by topoisomerase-VI-like complex, containing catalytic (e.g. SPO11) proteins auxiliary PRD3) proteins. in chromatin loops tethered linear axis, but interrelationship DSB-promoting factors axis is not fully...
Abstract During meiosis chromosomes undergo DNA double-strand breaks (DSBs) that can be repaired using a homolog to produce crossovers, which creates genetic diversity. Meiotic recombination occurs coincident with pairing and polymerization of the meiotic axis synaptonemal complex (SC). REC8-cohesin is required connect organize polymerization. However, control REC8 loading along chromosomes, in relation chromatin, transcription recombination, not yet fully understood. Therefore, we performed...
Abstract The number of meiotic crossovers is tightly controlled and most depend on pro-crossover ZMM proteins, such as the E3 ligase HEI10. Despite importance HEI10 dosage for crossover formation, how transcription remains unexplored. In a forward genetic screen using sensitive fluorescent seed reporter in Arabidopsis thaliana we identify heat shock factor binding protein (HSBP) repressor numbers. Using genome-wide mapping cytogenetics, show that hsbp mutations or HSBP knockdowns increase...