A5044045681- DNA Repair Mechanisms
- Photosynthetic Processes and Mechanisms
- Genomics and Chromatin Dynamics
- Microtubule and mitosis dynamics
- Mitochondrial Function and Pathology
- Chromosomal and Genetic Variations
- Epigenetics and DNA Methylation
- Soybean genetics and cultivation
- Nuclear Structure and Function
- Cancer therapeutics and mechanisms
- Recommender Systems and Techniques
- DNA and Nucleic Acid Chemistry
- Gestational Trophoblastic Disease Studies
- Insect Resistance and Genetics
- Genetic diversity and population structure
- Plant nutrient uptake and metabolism
- Plant Reproductive Biology
- Plant Molecular Biology Research
- Fungal and yeast genetics research
- Carcinogens and Genotoxicity Assessment
- Heat shock proteins research
- Diabetes and associated disorders
- RNA modifications and cancer
- Research on Leishmaniasis Studies
- Plant biochemistry and biosynthesis
Institut Jean-Pierre Bourgin
2009-2024
AgroParisTech
2012-2024
Université Paris-Saclay
2016-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2018-2024
Centre National de la Recherche Scientifique
2014-2018
A partner protein for meiotic snip Eukaryotes generate germ cells through recombination. This process initiates breaks in genomic DNA catalyzed by the SPO11 protein. Vrielynck et al. and Robert discover that SPO11, like topoisomerase VI enzymes, interacts with a (see Perspective Bouuaert Keeney). is required proper recombination found wide range of eukaryotes, suggesting it universal feature essential step. Science , this issue p. 939 943 ; see also 916
In numerous species, the formation of meiotic crossovers is largely under control a group proteins known as ZMM. Here, we identified new ZMM protein, HEI10, RING finger-containing protein that well conserved among species. We show HEI10 structurally and functionally related to yeast Zip3 it absolutely required for class I crossover (CO) in Arabidopsis thaliana. Furthermore, present foci on chromosome axes synaptonemal complex central element until pachytene. Then, from pachytene diakinesis,...
Significance Meiotic recombination promotes genetic diversity by shuffling parental chromosomes. As observed the very first geneticists, crossovers inhibit formation of another crossover nearby, an elusive phenomenon called interference. Another intriguing observation is heterochiasmy, marked difference in male and female rates many species. Here, we show that synaptonemal complex, a structure zips homologous chromosomes together during meiosis, essential for interference Arabidopsis . This...
Meiotic recombination is initiated by the formation of numerous DNA double-strand breaks (DSBs) catalysed widely conserved Spo11 protein. In Saccharomyces cerevisiae, requires nine other proteins for meiotic DSB formation; however, unlike Spo11, few these are across kingdoms. order to investigate this step in higher eukaryotes, we took advantage a high-throughput mutant screen carried out model plant Arabidopsis thaliana. A collection 55,000 lines was screened, and spo11-like mutations,...
During the leptotene stage of prophase I meiosis, chromatids become organized into a linear looped array via protein axis that forms along loop bases. Establishment is essential for subsequent synapsis homologous chromosome pairs and progression recombination to form genetic crossovers. Here, we describe ASYNAPTIC4 (ASY4), meiotic in Arabidopsis (Arabidopsis thaliana). ASY4 small coiled-coil exhibits limited sequence similarity with carboxyl-terminal region ASY3. We used enhanced yellow...
In the current meiotic recombination initiation model, SPO11 catalytic subunits associate with MTOPVIB to form a Topoisomerase VI-like complex that generates DNA double strand breaks (DSBs). Four additional proteins, PRD1/AtMEI1, PRD2/AtMEI4, PRD3/AtMER2 and plant specific DFO are required for DSB formation. Here we show (i) PRD1 provide link between sub-complex other (ii) PRD3/AtMER2, while localized axis, does not assemble canonical pre-DSB but establishes direct DSB-forming resection...
Crossovers (COs) are at the origin of genetic variability, occurring across successive generations, and they also essential for correct segregation chromosomes during meiosis. Their number position precisely controlled, however mechanisms underlying these controls poorly understood. Neddylation/rubylation is a regulatory pathway posttranslational protein modification that required numerous cellular processes in eukaryotes, but has not yet been linked to homologous recombination. In screen...
Crossovers (CO) shuffle genetic information and physically connect homologous chromosomal pairs, ensuring their balanced segregation during meiosis. COs arising from the major class I pathway require activity of well-conserved group ZMM proteins, which, in conjunction with MLH1, facilitate maturation DNA recombination intermediates specifically into COs. The HEI10 Interacting Protein 1 (HEIP1) was identified rice proposed to be a new, plant-specific member group. Here, we establish decipher...
Abstract Meiotic rapid prophase chromosome movements (RPMs) require connections between the chromosomes and cytoskeleton, involving SUN (Sad1/UNC-84)-domain-containing proteins at inner nuclear envelope (NE). RPMs remain significantly understudied in plants, with respect to their importance regulation of meiosis. Here, we demonstrate that Arabidopsis thaliana meiotic centromeres undergo (up 500 nm/s) uncoordinated during zygotene pachytene stages. These centromere are not affected by altered...
Genetic screens have been crucial for deciphering many important biological processes, including meiosis. In Arabidopsis thaliana, previous forward likely identified almost all the meiotic genes that when mutated lead to a pronounced decrease in fertility. However, increasing number of reverse genetics studies play roles meiosis, but do not exhibit strong phenotypes mutated, suggests there are still with function waiting be discovered. this study, we produced 897 A. thaliana homozygous...
Summary Two recombinases, RAD51 and DMC1, catalyze meiotic break repair to ensure crossovers (COs) between homologous chromosomes (interhomolog) rather than sisters (intersister). FIDGETIN‐LIKE‐1 (FIGL1) downregulates both recombinases. However, the understanding of how FIGL1 functions in remains limited. Here, we discover new genetic interactions Arabidopsis thaliana that are important vivo determinants outcome. In figl1 mutants, compromising RAD51‐dependent repair, either through loss...
Meiotic crossovers (COs) are important for reshuffling genetic information between homologous chromosomes and they essential their correct segregation. COs unevenly distributed along the underlying mechanisms controlling CO localization not well understood. We previously showed that meiotic mis-localized in absence of AXR1, an enzyme involved neddylation/rubylation protein modification pathway Arabidopsis thaliana. Here, we report axr1-/-, male meiocytes show a strong defect chromosome...
Abstract Two recombinases, RAD51 and DMC1, catalyze meiotic break repair to ensure crossovers (COs) between homologous chromosomes (interhomolog) rather than sisters (intersister). FIDGETIN-LIKE-1 (FIGL1) downregulates both recombinases. However, the understanding of FIGL1 functions in remains limited. Here, we discover new genetic interactions Arabidopsis thaliana that are important vivo determinants outcome. In figl1 , compromising RAD51-dependent by either losing paralogs (RAD51B or...
Abstract Meiotic rapid prophase chromosome movements (RPMs) require connections between the chromosomes and cytoskeleton, involving SUN (Sad1/UNC-84)-domain-containing proteins at inner nuclear envelope (NE). RPMs remain significantly understudied in plants, notably with respect to their importance regulation of meiosis. Here, we demonstrate that Arabidopsis thaliana meiotic centromeres undergo (up 500 nm/s) uncoordinated during zygotene pachytene stages. are not affected by altered...