A5085358039- DNA Repair Mechanisms
- Chromosomal and Genetic Variations
- Photosynthetic Processes and Mechanisms
- Fungal and yeast genetics research
- CRISPR and Genetic Engineering
- Genomics and Chromatin Dynamics
- Plant Genetic and Mutation Studies
- Genetic Mapping and Diversity in Plants and Animals
- Bacterial Genetics and Biotechnology
- Microtubule and mitosis dynamics
- Plant Virus Research Studies
- Plant nutrient uptake and metabolism
- Cancer therapeutics and mechanisms
- Polyomavirus and related diseases
- Plant tissue culture and regeneration
- Mitochondrial Function and Pathology
- Plant Disease Resistance and Genetics
- RNA and protein synthesis mechanisms
- Carcinogens and Genotoxicity Assessment
- Epigenetics and DNA Methylation
- Soybean genetics and cultivation
- Plant Molecular Biology Research
- Pancreatic function and diabetes
- Gestational Trophoblastic Disease Studies
- Redox biology and oxidative stress
Institut Jean-Pierre Bourgin
2012-2023
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2018-2023
AgroParisTech
2011-2023
Université Paris-Saclay
2016-2023
Centre National de la Recherche Scientifique
1999-2023
Institut National de la Recherche Agronomique
2005-2014
Institut National de la Recherche Agronomique du Niger
2012
Institut Curie
1999-2002
The Honourable Society of Lincoln's Inn
1998-1999
Laboratoire de Génétique Cellulaire
1999
In most species, crossovers (COs) are essential for the accurate segregation of homologous chromosomes at first meiotic division. Their number and location tightly regulated. Here, we report a detailed, genome-wide characterization rate localization COs in Arabidopsis thaliana, male female meiosis. We observed dramatic differences between meiosis which included: (i) genetic map length; 575 cM versus 332 respectively; (ii) CO distribution patterns: rates were very high both ends each...
A partner protein for meiotic snip Eukaryotes generate germ cells through recombination. This process initiates breaks in genomic DNA catalyzed by the SPO11 protein. Vrielynck et al. and Robert discover that SPO11, like topoisomerase VI enzymes, interacts with a (see Perspective Bouuaert Keeney). is required proper recombination found wide range of eukaryotes, suggesting it universal feature essential step. Science , this issue p. 939 943 ; see also 916
The success of the first meiotic division relies (among other factors) on formation bivalents between homologous chromosomes, monopolar orientation sister kinetochores at metaphase I and maintenance centromeric cohesion until onset anaphase II. cohesin subunit, Rec8 has been reported to be one key players in these processes, but its precise role kinetochore is still under debate. By contrast, much less known about non-SMC Scc3. We report identification characterisation AtSCC3, sole...
Crossover (CO) is a key process for the accurate segregation of homologous chromosomes during first meiotic division. In most eukaryotes, recombination not homogeneous along chromosomes, suggesting tight control location events. We genotyped 71 single nucleotide polymorphisms (SNPs) covering entire chromosome 4 Arabidopsis thaliana on 702 F2 plants, representing 1404 meioses and allowing detection 1171 COs, to study CO localization in higher plant. The genetic rates varied from 0 cM/Mb near...
Meiosis is a specialized eukaryotic cell division that generates haploid gametes required for sexual reproduction. During meiosis, homologous chromosomes pair and undergo reciprocal genetic exchange, termed crossover (CO). Meiotic CO frequency varies along the physical length of determined by hierarchical mechanisms, including epigenetic organization, example methylation DNA histones. Here we investigate role in determining patterns Arabidopsis thaliana chromosomes. In A. pericentromeric...
Meiotic crossover frequency varies extensively along chromosomes and is typically concentrated in hotspots. As recombination increases genetic diversity, hotspots are predicted to occur at immunity genes, where variation may be beneficial. A major component of plant recognition pathogen Avirulence (Avr) effectors by resistance (R) genes that encode NBS-LRR domain proteins. Therefore, we sought test whether would overlap with meiotic using experimental genetics Arabidopsis thaliana. tend...
In many species, sex-related differences in crossover (CO) rates have been described at chromosomal and regional levels. this study, we determined the CO distribution along entire Arabidopsis thaliana Chromosome 4 (18 Mb) male female meiosis, using high density genetic maps built on large backcross populations (44 markers, >1,300 plants). We observed dramatic between map lengths that were calculated as 88 cM 52 cM, respectively. This difference is remarkably parallel to total synaptonemal...
The vast majority of meiotic recombination events (crossovers (COs) and non-crossovers (NCOs)) cluster in narrow hotspots surrounded by large regions devoid recombinational activity. Here, using a new molecular approach plants, called "pollen-typing", we detected characterized hundreds CO NCO molecules two different hotspot Arabidopsis thaliana. This analysis revealed that COs are concentrated few kilobases where their rates reach up to 50 times the genome average. themselves tend less than...
In the current meiotic recombination initiation model, SPO11 catalytic subunits associate with MTOPVIB to form a Topoisomerase VI-like complex that generates DNA double strand breaks (DSBs). Four additional proteins, PRD1/AtMEI1, PRD2/AtMEI4, PRD3/AtMER2 and plant specific DFO are required for DSB formation. Here we show (i) PRD1 provide link between sub-complex other (ii) PRD3/AtMER2, while localized axis, does not assemble canonical pre-DSB but establishes direct DSB-forming resection...
Tay-Sachs disease is an autosomal recessive genetic caused by a deficiency in beta-hexosaminidase A. We have characterized new mutation Tunisian patient displaying late infantile form of disease. Northern blot analysis patient's fibroblast total RNAs shows broad, fast migrating band the region normal alpha transcripts. The mRNA coding for subunit was first reverse transcribed and then amplified four overlapping segments spanning entire sequence polymerase chain reaction. found products...
Summary During meiotic prophase I chromosomes undergo dramatic conformational changes that accompany chromosome condensation, pairing and recombination between homologs. These include the anchoring of telomeres to nuclear envelope their clustering form a bouquet. In plants, these events have been studied illustrated in intact meiocytes species with large genomes. Arabidopsis thaliana is an excellent genetic model which major molecular pathways control synapsis homologs uncovered. Yet study...
Different modes of in vivo repair double-strand breaks (DSBs) have been described for various organisms: the recombinational DSB (DSBR) mode, single-strand annealing (SSA) and end-to-end joining. To investigate these Saccharomyces cerevisiae, we examined fate vitro linearized replicative plasmids during transformation with respect to several parameters. We found that (i) efficiencies both intramolecular intermolecular linear plasmid are homology dependent (according amount DNA used [100 ng...