A5081176696- Plant Molecular Biology Research
- Chromosomal and Genetic Variations
- Plant Reproductive Biology
- Plant Genetic and Mutation Studies
- Plant nutrient uptake and metabolism
- Wheat and Barley Genetics and Pathology
- Legume Nitrogen Fixing Symbiosis
- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- Soybean genetics and cultivation
- Photosynthetic Processes and Mechanisms
- RNA Research and Splicing
- Plant Gene Expression Analysis
- Plant tissue culture and regeneration
- DNA Repair Mechanisms
- Meat and Animal Product Quality
- RNA modifications and cancer
- Seed Germination and Physiology
- Protist diversity and phylogeny
- Adipokines, Inflammation, and Metabolic Diseases
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Carcinogens and Genotoxicity Assessment
- Bioenergy crop production and management
- Data Stream Mining Techniques
- Recommender Systems and Techniques
Institute of Science and Technology Austria
2023-2025
Xi'an Jiaotong University
2024
John Innes Centre
2015-2023
Norwich Research Park
2015-2022
Alibaba Group (China)
2022
Zhejiang University
2022
University of Wisconsin–Madison
2019
Gansu Agricultural University
2014
University of California, Berkeley
2012-2013
Huashan Hospital
2009-2011
Intergenerational Transposable Shutdown elements (TEs) are a potential threat, especially to the germline genome. In many eukaryotes, TEs shut down by DNA methylation and/or small-RNA–mediated silencing. Therefore, it seems counterintuitive that results obtained Ibarra et al. (p. 1360 ) on Arabidopsis showed in cells of this plant's sexual apparatus, small demethylated DEMETER (DME) glycosylase and become activated. But turns out activation triggers formation small-interfering RNAs, which...
Significance Flowering plant reproduction involves two fertilization events: a sperm–egg fusion that forms the embryo, and sperm–central cell nutritive extraembryonic endosperm. Chromosomes in endosperm, other tissues are modified by methylation, covalent addition to cytosine DNA regulates gene expression. Maternal endosperm chromosomes inherited from central display pattern of extensive demethylation, which is essential for seed development Arabidopsis thaliana . Demethylation presumed...
Germline defense against transposons Genomes of germ cells present an existential vulnerability to organisms because cell mutations will propagate future generations. Transposable elements are one source such mutations. In the small flowering plant Arabidopsis , Long et al. found that genome methylation in male germline is directed by interfering RNAs (siRNAs) imperfectly transcribed from (see Perspective Mosher). These siRNAs silence and establish inherited patterns sperm, thus maintaining...
Abstract Background Plant and animal embryogenesis have conserved distinct features. Cell fate transitions occur during in both plants animals. The epigenomic processes regulating plant remain largely elusive. Results Here, we elucidate chromatin transcriptomic dynamics of the most cultivated crop, hexaploid wheat. Time-series analysis reveals stage-specific proximal–distal accessibility concordant with transcriptome changes. Following fertilization, remodeling kinetics H3K4me3, H3K27ac,...
Abstract In the eukaryotic nucleus, heterochromatin forms highly condensed, visible foci known as (HF). These HF are enriched with linker histone H1, a key player in condensation and silencing. However, it is unknown how H1 aggregates condenses heterochromatin. this study, we established that facilitates by enhancing inter- intrachromosomal interactions between within heterochromatic regions of Arabidopsis (Arabidopsis thaliana) genome. We demonstrated drives formation via phase separation,...
Transposable elements (TEs), the movement of which can damage genome, are epigenetically silenced in eukaryotes. Intriguingly, TEs activated sperm companion cell - vegetative (VC) flowering plant Arabidopsis thaliana. However, extent and mechanism this activation unknown. Here we show that about 100 heterochromatic VCs, mostly by DEMETER-catalyzed DNA demethylation. We further demonstrate DEMETER access to some these is permitted natural depletion linker histone H1 VCs. Ectopically expressed...
Abstract Sperm chromatin is typically transformed by protamines into a compact and transcriptionally inactive state 1,2 . cells of flowering plants lack protamines, yet they have small, active nuclei with condensed through an unknown mechanism 3,4 Here we show that histone variant, H2B.8, mediates sperm nuclear condensation in Arabidopsis thaliana Loss H2B.8 causes enlarged dispersed chromatin, whereas ectopic expression somatic produces smaller aggregated chromatin. This result demonstrates...
Cytosine DNA methylation regulates the expression of eukaryotic genes and transposons. Methylation is copied by methyltransferases after replication, which results in faithful transmission patterns during cell division and, at least flowering plants, across generations. Transgenerational inheritance mediated a small group cells that includes gametes their progenitors. However, usually analyzed somatic tissues do not contribute to next generation, mechanisms transgenerational are inferred...
Cytosine methylation within CG dinucleotides (mCG) can be epigenetically inherited over many generations. Such inheritance is thought to mediated by a semiconservative mechanism that produces binary present/absent patterns. However, we show here that, in Arabidopsis thaliana h1ddm1 mutants, intermediate heterochromatic mCG stably across generations and quantitatively associated with transposon expression. We develop mathematical model estimates the rates of maintenance failure de novo at...
The four microsporangia of the flowering plant anther develop from archesporial cells in L2 primordium. Within each microsporangium, developing microsporocytes are surrounded by concentric monolayers tapetal, middle layer and endothecial cells. How this intricate array tissues, containing relatively few cells, is established an organ possessing no formal meristems poorly understood. We describe here pivotal role LRR receptor kinase EXCESS MICROSPOROCYTES 1 (EMS1) forming monolayer tapetal...
Abstract Dynamic DNA methylation represses transposable elements (TEs) and regulates gene activity, playing a pivotal role in plant development. Although substantial progress has been made understanding reprogramming during germline development Arabidopsis thaliana , whether similar mechanisms exist other dicot plants remains unclear. Here, we analyzed levels meiocytes, microspores, pollens of Brassica Rapa using whole-genome bisulfite sequencing (WGBS). Global analysis revealed CHH compared...
Significance Epigenetic regulation of gene expression is associated with switching between chromatin states characterized by distinct histone modifications. Polycomb/Trithorax involves the mutually exclusive H3K27me3/H3K36me3 modifications, but how these are faithfully inherited through DNA replication, yet can switch from one to another, still poorly understood. One mechanism that would aid association methyltransferases factors demethylating opposing modification. Here, we show an...
SNC1 (SUPPRESSOR OF NPR1, CONSTITUTIVE 1) is one of a suite intracellular Arabidopsis NOD-like receptor (NLR) proteins which, upon activation, result in the induction defense responses. However, molecular mechanisms underlying NLR activation and subsequent provocation immune responses are only partially characterized. To identify negative regulators NLR-mediated immunity, forward genetic screen was undertaken to search for enhancers dwarf, autoimmune gain-of-function snc1 mutant. avoid...
Activation of cell-surface and intracellular receptor-mediated immunity results in rapid transcriptional reprogramming that underpins disease resistance. However, the mechanisms by which co-activation both immune systems lead to changes are not clear. Here, we combine RNA-seq ATAC-seq define gene expression chromatin accessibility. or immunity, both, increases accessibility at induced defence genes. Analysis data combined with publicly available information on transcription factor...
The study of RNAs has become one the most influential research fields in contemporary biology and biomedicine. In last few years, new sequencing technologies have produced an explosion exciting discoveries field but also given rise to many open questions. Defining these questions, together with old, long-standing gaps our knowledge, is spirit this article. breadth topics within RNA vast, every aspect molecules contains countless Here, we asked 12 groups discuss their compelling question...
Geranylgeranyl diphosphate synthase (GGPPS, EC: 2.5.1.29) catalyzes the biosynthesis of geranylgeranyl (GGPP), which is a key precursor for ginkgolide biosynthesis. Here we reported first time cloning new full-length cDNA encoding GGPPS from living fossil plant Ginkgo biloba. The G. biloba (designated as GbGGPPS) was 1657 bp long and contained 1176 open reading frame 391 amino acid protein. Comparative analysis showed that GbGGPPS possessed 79 transit peptide at its N-terminal, directed to...
Abstract Cytosine methylation within CG dinucleotides (mCG) can be epigenetically inherited over many generations. Such inheritance is thought to mediated by a semiconservative mechanism that produces binary present/absent patterns. However, we show here in Arabidopsis thaliana h1ddm1 mutants, intermediate heterochromatic mCG stably across generations and quantitatively associated with transposon expression. We develop mathematical model estimates the rates of maintenance failure de novo at...