A5101657694- Plant biochemistry and biosynthesis
- Organ Transplantation Techniques and Outcomes
- Natural product bioactivities and synthesis
- Cancer therapeutics and mechanisms
- Genomics and Phylogenetic Studies
- Cancer Treatment and Pharmacology
- Coagulation and Flocculation Studies
- Kidney Stones and Urolithiasis Treatments
- Maritime Security and History
- Anesthesia and Neurotoxicity Research
- Nutritional Studies and Diet
- Mesenchymal stem cell research
- Enzyme Structure and Function
- Diet and metabolism studies
- Electrical and Bioimpedance Tomography
- Colorectal Cancer Treatments and Studies
- Vitamin D Research Studies
- Cardiac Ischemia and Reperfusion
- Body Composition Measurement Techniques
- Liver Disease and Transplantation
- Porphyrin Metabolism and Disorders
- Biomedical Research and Pathophysiology
- Autophagy in Disease and Therapy
- Metabolism and Genetic Disorders
- Nutrition and Health in Aging
University of Central Lancashire
2024
University of Greenwich
2024
Institute of Biomedical and Genetic Engineering
2023
University of Toronto
2023
St. Michael's Hospital
2023
Nanyang Technological University
2022-2023
COMSATS University Islamabad
2013-2023
Plants accumulate a vast array of secondary metabolites, which constitute natural resource for pharmaceuticals. Oldenlandia corymbosa belongs to the Rubiaceae family, and has been used in traditional medicine treat different diseases, including cancer. However, active metabolites plant, their biosynthetic pathway mode action cancer are unknown. To fill these gaps, we exposed this plant eight stress conditions combined omics data capturing gene expression, metabolic profiles, anti-cancer...
Kinases have proven valuable targets in successful cancer drug discovery projects, but not yet for malignant brain tumors where type-II inhibition of cyclin-dependent kinase 5 (CDK5) stabilizing the DFG-out inactive state has potential design selective and clinically efficient candidates. In absence crystallographic evidence a CDK5 protein-ligand complex, first time, model was designed using metadynamics/molecular dynamics simulations. Glide docking ZINC15 biogenic database identified...
More than one billion people around the globe are suffering from severe to moderate vitamin D deficiency. In Pakistan, 53.5% suffer The purpose of this study is develop fortified yogurt with two varying concentrations D3, aiming observe how these impact physicochemical and sensory characteristics products. A total 750 mL pasteurized milk was divided into three equal batches. For T1 T2, 50 µL 66.67 emulsified D3 were added at °C, respectively. Yogurt samples prepared in triplicates underwent...
Hemorrhagic shock-resuscitation (HSR) following trauma contributes to organ dysfunction by causing ischemia–reperfusion injury (IRI). We previously showed that 'remote ischemic preconditioning' (RIPC) exerted multi-organ protection from IRI. Maintenance of mitochondrial quality clearance dysfunctional mitochondria via mitophagy is vital in restoring integrity. hypothesized parkin-dependent played a role RIPC-induced hepatoprotection HSR. The hepatoprotective effect RIPC murine model HSR-IRI...
Sarcopenia is the loss of muscle mass and strength, or both, physiological functions through aging. Early detection weakness results in better care interventions regarding dietary habits protein intake older adults. The objective this study was to investigate prevalence sarcopenia a Pakistani population correlate lifestyle with sarcopenia. A sample size 150 individuals, both male female, age sixty above used. Cardiovascular disease renal failure patients were excluded. research field...
Abstract Natural products from traditional medicinal plants are valuable candidates for clinical cancer therapy. Plants the Oldenlandia-Hedyotis complex popular ingredients of Traditional Chinese Medicine (TCM), however a major hurdle in plant bioprospecting process TCM is that active metabolites, their biosynthetic pathways, and mode action often unknown. We show Oldenlandia corymbosa extracts against breast cell lines. To study genes involved biosynthesis compounds this plant, we assembled...
Abstract Background Primary Hyperoxaluria Type 1 (PH1) is an autosomal recessive genetic disorder triggered by a mutation in the alanine glyoxylate aminotransferase (AGXT) gene. Early detection of PH1 pre-requisite as it causes End Stage Renal Disease (ESRD) most patients early stages. An eleven years old girl with history kidney disease and stones phenotypic characteristics was brought to laboratory. A c.568G>A AGXT gene, which responsible for PH1, found homozygous condition. Further...
Abstract AIMS Design and statistically optimize an in silico screening protocol; apply the protocol to a compound database; vitro validation of selected inhibitors (isolated CDK5 enzyme binding assay). METHOD Two large databases, ZINC15 Analyticon Discovery, were used screen for potential ATP-binding site inhibitors. They first filtered using generated pharmacophore model then virtually screened Glide SP docking with solved CDK5-p25 structure (PDB: 3O0G). The candidates validated assays...