A5011477132- Biochemical and Structural Characterization
- Antimicrobial Peptides and Activities
- Peptidase Inhibition and Analysis
- Glycosylation and Glycoproteins Research
- Ginseng Biological Effects and Applications
- Natural product bioactivities and synthesis
- Microbial Natural Products and Biosynthesis
- RNA Interference and Gene Delivery
- Plant biochemistry and biosynthesis
- Botanical Research and Applications
- Chemical Synthesis and Analysis
- Carbohydrate Chemistry and Synthesis
- ATP Synthase and ATPases Research
- Genomics and Phylogenetic Studies
- Pharmacological Effects of Natural Compounds
- Adipose Tissue and Metabolism
- Phytochemical compounds biological activities
- Autophagy in Disease and Therapy
- Toxin Mechanisms and Immunotoxins
- Enzyme Production and Characterization
- Transgenic Plants and Applications
- Marine Sponges and Natural Products
- Signaling Pathways in Disease
- Botanical Research and Chemistry
- Advancements in Transdermal Drug Delivery
Xi’an Jiaotong-Liverpool University
2023-2025
Nanyang Technological University
2015-2024
Macrocyclization is a valuable tool for drug design and protein engineering. Although various methods have been developed to prepare macrocycles, general efficient strategy needed. Here we report highly method using butelase 1 macrocyclize peptides proteins ranging in sizes from 26 >200 residues. We achieved cyclizations that are 20,000 times faster than sortase A, the most widely used ligase cyclization. The reactions completed within minutes with up 95% yields.
Abstract Disulfides in peptides and proteins are essential for maintaining a properly folded structure. Their oxidative folding is invariably performed an aqueous‐buffered solution. However, this process often slow can lead to misfolded products. Here, we report novel concept strategy that bio‐inspired mimic protein disulfide isomerase (PDI) by accelerating exchange rates many thousand‐fold. The proposed termed organic under solvents yield correctly cysteine‐rich microproteins...
Plants accumulate a vast array of secondary metabolites, which constitute natural resource for pharmaceuticals. Oldenlandia corymbosa belongs to the Rubiaceae family, and has been used in traditional medicine treat different diseases, including cancer. However, active metabolites plant, their biosynthetic pathway mode action cancer are unknown. To fill these gaps, we exposed this plant eight stress conditions combined omics data capturing gene expression, metabolic profiles, anti-cancer...
(1) Background: Doxorubicin (DOX) is a frontline chemotherapeutic, but its side effects from oxidative stress leading to cardiotoxicity, pose significant challenges clinical use. We recently discovered novel family of proteolysis-resistant, cystine-dense, and cell-penetrating microproteins Panax ginseng that we term ginsentides. Ginsentides, such as the 31-residue TP1 coordinate multiple biological systems prevent vascular dysfunction endoplasmic reticulum induced by internal external...
Abstract Plant knottins are of therapeutic interest due to their high metabolic stability and inhibitory activity against proteinases involved in human diseases. The only knottin-type proteinase inhibitor porcine pancreatic elastase was first identified from the squash family 1989. Here, we report identification characterization a neutrophil Hibiscus sabdariffa Malvaceae family. Combining proteomic transcriptomic methods, panel novel cysteine-rich peptides, roseltides (rT1-rT8), which range...
Chitin-binding hevein-like peptides (CB-HLPs) belong to a family of cysteine-rich that play important roles in plant stress and defense mechanisms. CB-HLPs are ribosomally synthesized known be bioprocessed from the following two types three-domain CB-HLP precursor architectures: cargo-carrying non-cargo-carrying. Here, we report identification characterization chenotides biosynthesized third type precursors, which cleavable hololectins quinoa (Chenopodium quinoa) family. Chenotides...
Cysteine-rich peptides (CRPs) play important host-defense roles in plants. However, information concerning CRPs the Cactaceae (cactus) family is limited, with only a single cactus-derived CRP described to date. Here, we report identification of 15 novel three different precursor architectures, bleogens pB1-15 from Pereskia bleo family. By combining proteomic and transcriptomic methods, showed that prototype, bleogen pB1, contained 36 amino acid residues, six-cysteine motif typical...
Ginseng, a popular and valuable traditional medicine, has been used for centuries to maintain health treat disease. Here we report the discovery characterization of ginsentides, novel family cysteine glycine-rich peptides derived from three most widely-used ginseng species: Panax ginseng, quinquefolius, notoginseng. Using proteomic transcriptomic methods, identified 14 TP1-TP14 which consist 31-33 amino acids whose expression profiles are species- tissues-dependent. Ginsentides have an...
Butelase-1, an asparaginyl endopeptidase or legumain, is the prototypical and fastest known Asn/Asp-specific peptide ligase. It highly useful for engineering macrocyclization of peptides proteins. However, certain biochemical properties applications naturally occurring recombinant butelase-1 remain unexplored. Here we report methods to increase yield natural bacterial expressed how they can be used improve stability activity two important industrial enzymes, lipase phytase, by end-to-end...
Heveins and hevein-containing (hev-) lectins play important roles in stress pathogenic responses plants but cause health concerns humans. Hev-hololectins contain multiple modular hev-peptide domains are abundantly present cereals pseudocereals. However, it is unclear why some cereal hev-hololectins presented as different forms of proteolytically processed proteoforms. Here we show the precursor architectures lead to processing mechanisms give either hololectins or hevein-like peptides. We...
Hypoxia-induced vascular endothelial dysfunction (VED) is a significant contributor to several severe human diseases, including heart disease, stroke, dementia, and cancer. However, current treatment options for VED are limited due the lack of understanding underlying disease mechanisms therapeutic leads. We recently discovered heat-stable microprotein in ginseng, called ginsentide TP1, that has been shown reduce cardiovascular models. In this study, we use combination functional assays...
Here, we report the discovery of first plant-derived and noncanonical epidermal growth factor receptor (EGFR) agonist, 36-residue bleogen pB1 from Pereskia bleo Cactaceae family. We show that is a low-affinity EGFR agonist using suite chemical, biochemical, cellular, animal experiments which include incisor eruption wound-healing mouse models. A focused positional scanning library Ala- d-amino acid scans yielded high-affinity analog, [K29k]pB1, with 60-fold-improved affinity mitogenicity....
Abstract Ginseng has been known as a “cure-all” traditional medicine to treat various illnesses and an adaptogen relieve stress. However, the active compounds of ginseng are small-molecule metabolites. Here we report ginsentides, which disulfide-dense, super-stable cell-penetrating peptides with 31–33 amino acids, adaptogens that restore homeostasis in response Using mass spectrometry-based target identification functional studies, show ginsentides promote vasorelaxation by producing nitric...
Stress and illness connection is complex involves multiple physiological systems. Panax ginsengs, reputed for their broad-spectrum "cure-all" effect, are widely prescribed to treat stress related illnesses. However, the identity of ginseng's medicinal compounds that relieve remains unresolved. Here, we identify ginsentides as principal bioactives coordinate systems restore homeostasis in response stress. Ginsentides disulfide-rich, cell-penetrating proteolytic-stable microproteins. Using...
Corneal scarring reduces corneal transparency, compromises vision, and is a major cause of vision loss worldwide. Epidermal growth factor (EGF), which the prototypic member EGF receptor (EGFR) agonists, present in tears to provide repair regeneration. Recently, we discovered bleogen pB1 cactus plant Pereskia bleo showed that it non-canonical hyperstable EGFR agonist with EGF-like wound healing properties for diabetic rats. Here, apply accelerate To assess effects pB1, induced an acute alkali...
Abstract Hypoxia-induced vascular endothelial dysfunction (VED) is a significant contributor to several severe human conditions, including heart disease, stroke, dementia, and cancer. However, current treatment options for VED are limited due lack of understanding the underlying disease mechanisms therapeutic leads. We recently discovered heat-stable microprotein in ginseng, known as ginsentide TP1 that has been shown reduce cardiovascular models. In this study, we use combination functional...
Coffee processing generates a huge amount of waste that contains many natural products. Here, we report the discovery panel novel cell-penetrating and metal ion-binding microproteins designated coffeetide cC1a-c cL1-6 from husk two popular coffee plants,
Insulin-resistant diabetes is a common metabolic disease with serious complications. Treatments directly addressing the underlying molecular mechanisms involving insulin resistance would be desirable. Our laboratory recently identified proteolytic-resistant cystine-dense microprotein from huáng qí (Astragalus membranaceus) called α-astratide aM1, which shares high sequence homology to leginsulins. Here we show that aM1 cell-penetrating mimetic, enters cells by endocytosis, and activates...
Abstract Disulfides in peptides and proteins are essential for maintaining a properly folded structure. Their oxidative folding is invariably performed an aqueous‐buffered solution. However, this process often slow can lead to misfolded products. Here, we report novel concept strategy that bio‐inspired mimic protein disulfide isomerase (PDI) by accelerating exchange rates many thousand‐fold. The proposed termed organic under solvents yield correctly cysteine‐rich microproteins...
Natural products have always played a vital role in the search for novel drugs, and their exploration continues to captivate researchers field of drug discovery development [...]
Coffee processing generates a huge amount of waste which contains many natural products. Here, we report the discovery panel novel cell-penetrating and metal-ion binding microproteins designated coffeetide cC1a-c cL1-6 from husk two popular coffee plants, Coffea canephora liberica, respectively. Combining sequence determination database search, showed that prototypic cC1a is 37-residue, eight-cysteine microprotein with hevein-like cysteine motif but without chitin-binding domain. NMR...