A5062977612- Biochemical and Structural Characterization
- Peptidase Inhibition and Analysis
- Spectroscopy Techniques in Biomedical and Chemical Research
- interferon and immune responses
- Traditional Chinese Medicine Analysis
- Pineapple and bromelain studies
- Transgenic Plants and Applications
- Enzyme Production and Characterization
- Immune Response and Inflammation
- 3D Printing in Biomedical Research
- Identification and Quantification in Food
- Phytochemical compounds biological activities
- Invertebrate Immune Response Mechanisms
- Mobile Crowdsensing and Crowdsourcing
- Metabolomics and Mass Spectrometry Studies
- Drug Solubulity and Delivery Systems
- Distributed Sensor Networks and Detection Algorithms
- Virus-based gene therapy research
- Microbial Natural Products and Biosynthesis
- Autophagy in Disease and Therapy
- Cell death mechanisms and regulation
- Monoclonal and Polyclonal Antibodies Research
- Biofuel production and bioconversion
- Cancer-related molecular mechanisms research
- Plant-based Medicinal Research
Singapore-MIT Alliance for Research and Technology
2021-2025
Beijing University of Chinese Medicine
2023-2024
Nanyang Technological University
2017-2023
Hunan Normal University
2021
We demonstrate the feasibility of machine-learning aided UV absorbance spectroscopy for in-process microbial contamination detection during cell therapy product (CTP) manufacturing. This method leverages a one-class support vector machine to analyse spectra cultures and predict if sample is sterile or contaminated. label-free technique provides rapid output (< 30 minutes) with minimal preparation volume 1 mL). Spiking 7 organisms into mesenchymal stromal cells supernatant aliquots from 6...
Astragalus membranaceus root, Huang Qi in Chinese, is a popular medicinal herb traditionally used to regulate blood glucose. Herein, the identification and characterization of two families cysteine-rich peptides (CRPs), designated α- β-astratides, from A. roots are reported. Proteomic analysis showed that α-astratide aM1 β-astratide bM1 belong distinct CRP families. The six-cysteine-containing proline-rich displayed high sequence identity Pea Albumin 1 Subunit b (PA1b), while...
Abstract Cysteine‐rich peptides (CRPs), which are disulfide‐constrained with 3 to 5 disulfide bonds and molecular weights of 2 6 kDa, generally hyperstable resistant thermal, chemical, enzymatic degradation. Herein, the discovery characterization a novel suite CRPs, collectively named potentides pA1–pA16 from root medicinal herb Potentilla anserina L , described. Through combination proteomic transcriptomic methods, it is shown that 35‐residue potentide pA3, most abundant member potentides,...
Species misidentification and adulteration are major concerns in authenticating herbal medicines. Radix Astragali (RA), the roots of Astragalus membranaceus, is a traditional medicine used for treating diabetes. However, it often substituted by Hedysarum (RH), polybotrys from same plant family Fabaceae, which possesses different bioactivities. Current authentication methods, focusing on chemical composition differences medicines based small molecules, have limitations when these markers...
Monoclonal antibodies require careful formulation due to their inherent stability limitations. Polysorbates are commonly used stabilize mAbs, but they prone degradation, which results in unwanted impurities. KLEPTOSE
Controlling microbial risks in cell therapy products (CTPs) is important for product safety. Here, we identified the nicotinic acid (NA) to nicotinamide (NAM) ratio as a biomarker that detects broad spectrum of contaminants cultures. We separately added six different bacterial species into mesenchymal stromal and T culture found NA was uniquely present these bacteria-contaminated CTPs due conversion from NAM by nicotinamidases, which mammals lack. In cells inoculated with 1 × 104 CFUs/mL...
Coffee processing generates a huge amount of waste that contains many natural products. Here, we report the discovery panel novel cell-penetrating and metal ion-binding microproteins designated coffeetide cC1a-c cL1-6 from husk two popular coffee plants,
Grafting a bioactive peptide onto disulfide-rich scaffold is promising approach to improve its structure and metabolic stability. The ginkgo plant-derived β-ginkgotide β-gB1 highly unusual molecule: Small, hyperdisulfide, found only in selected ancient plants. It also contains conserved 16-amino-acid core with three interlocking disulfides, as well six-amino-acid inter-cysteine loop 2 suitable for grafting epitopes. However, very little known about this recently-discovered family of...
<title>Abstract</title> We demonstrate the use of machine-learning aided UV absorbance spectroscopy to detect contamination during cell therapy product (CTP) manufacturing. The proposed method leverages a one-class support vector machine model analyse spectra cultures and predict if sample is sterile or contaminated. This label-free non-invasive provides rapid output (30 minutes) with minimal preparation volume (<1mL). Spiking 7 bacterial species into mesenchymal stromal cells supernatant...
As large language models continue to scale up, the imperative for fault tolerance in distributed deep learning systems intensifies, becoming a focal area of AI infrastructure research. Checkpoint has emerged as predominant strategy, with extensive studies dedicated optimizing its efficiency. However, advent sparse Mixture-of-Experts (MoE) model presents new challenges traditional checkpoint techniques due substantial increase size, despite comparable computational demands dense models....
Coffee processing generates a huge amount of waste which contains many natural products. Here, we report the discovery panel novel cell-penetrating and metal-ion binding microproteins designated coffeetide cC1a-c cL1-6 from husk two popular coffee plants, Coffea canephora liberica, respectively. Combining sequence determination database search, showed that prototypic cC1a is 37-residue, eight-cysteine microprotein with hevein-like cysteine motif but without chitin-binding domain. NMR...
Huamaoyan Granules (HMYG) and Capsules (HMYC) are Chinese patent medicines with different dosage forms of the same prescription. Due to preparation process, chemical composition these varies greatly among forms, but there were few studies on difference comparison quality control them. In order improve effectiveness safety in its clinical application, an idea combining high performance liquid chromatography (HPLC) chemometrics was put forward study
Traditional Chinese medicine (TCM) is gaining popularity as an alternative medicine. A central element of TCM the use herbal medicine, which could be benefited by a novel authentication method. Astragalus membranaceus , belongs to Fabaceae family, flowering plant native China. Its root, commonly known Huang Qi, traditional used for treating diabetes, promoting Qi and boosting immune system. However, there another medicinal herb, Hedysarum polybotrys also sharing similar name (Hong Qi)...
We show a machine learning-aided UV spectroscopy-based method using aseptic instrumentation to detect metabolite NA, marker of microbial contamination. This potentially enables rapid, at-line contamination detection in cell manufacturing.