A5016947185- Nanoplatforms for cancer theranostics
- Advanced biosensing and bioanalysis techniques
- 3D Printing in Biomedical Research
- RNA Interference and Gene Delivery
- Natural Compound Pharmacology Studies
- Cell death mechanisms and regulation
- Cancer Cells and Metastasis
- Advanced Fiber Laser Technologies
- Biological Activity of Diterpenoids and Biflavonoids
- Zebrafish Biomedical Research Applications
- Microtubule and mitosis dynamics
- Cellular Mechanics and Interactions
- Luminescence and Fluorescent Materials
- Nonlinear Dynamics and Pattern Formation
- Nonlinear Photonic Systems
- Microfluidic and Bio-sensing Technologies
- Angiogenesis and VEGF in Cancer
- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- Bioactive Compounds and Antitumor Agents
- Immune Cell Function and Interaction
- Photonic and Optical Devices
- Natural product bioactivities and synthesis
- Phytoestrogen effects and research
- Neuroinflammation and Neurodegeneration Mechanisms
University of Macau
2016-2025
Shandong University
2025
Second Affiliated Hospital of Zhejiang University
2024
Chongqing Cancer Hospital
2020-2024
Chongqing University
2017-2024
Jiangxi University of Traditional Chinese Medicine
2024
University of Electronic Science and Technology of China
2023
Mianyang Central Hospital
2023
University of Chinese Academy of Sciences
2020
Harbin Medical University
2020
Molecules of siloles are almost non-fluorescent but their nanoaggregates highly emissive, exhibiting aggregation-induced emission (AIE). The AIE-active aggregates cytocompatible with living cells, stain cytoplasms HeLa cells indelibly without contaminating another type cell line in a co-culture system, and remain visible for long period time. Detailed facts importance to specialist readers published as "Supporting Information". Such documents peer-reviewed, not copy-edited or typeset. They...
Abstract Circulating tumor cells (CTCs) are the primary targets of cancer treatment as they cause distal metastasis. However, how CTCs response to exercise-induced high shear stress is largely unknown. To study effects hemodynamic microenvironment on CTCs, we designed a microfluidic circulatory system that produces exercise relevant stresses. We explore stresses breast with different metastatic abilities, ovarian, lung and leukemic origin. Three major findings were obtained. 1) High 60...
Human serum albumin (HSA) is a major protein component of blood plasma, and its assay obvious value to biological research. We, herein, present readily accessible fluorescent bioprobe for HSA detection quantitation. A nonemissive tetraphenylethene derivative named sodium 1,2-bis[4-(3-sulfonatopropoxyl)phenyl]-1,2-diphenylethene (BSPOTPE) induced emit by HSA, showing novel phenomenon aggregation-induced emission (AIE). The AIE enjoys broad working range (0−100 nM), low limit (down 1 superior...
Background and aims Pancreatic ductal adenocarcinoma (PDAC) is a leading cause of cancer-related death worldwide. Neurotransmitter-initiated signalling pathway profoundly implicated in tumour initiation progression. Here, we investigated whether dysregulated neurotransmitter receptors play role during pancreatic tumourigenesis. Methods The Cancer Genome Atlas Gene Expression Omnibus datasets were used to identify differentially expressed receptors. expression pattern gamma-aminobutyric acid...
Although organic semiconducting polymer nanoparticles (SPNs) have emerged as an important category of optical imaging agents, their application in molecular is still its infancy and faces many challenges. We herein report a straightforward one-pot synthetic approach to construct multilayered near-infrared (NIR) fluorescent SPNs with enhanced fluorescence optimized biodistribution for vivo imaging. In addition the SP core, middle silica protection layer outer poly(ethylene glycol) (PEG)...
Liver is one of the most preferred destinations distant metastasis in gastric cancer (GC). As effective treatment still limited, prognosis GC patients bearing liver poor. We filter out lysyl oxidase (LOX) to study its function tumor microenvironment (TME) and seek for potential therapeutic targets.Transcription analysis on 6 cases with respective paired primary tumors adjacent normal livers was performed. The filtration LOX done using 5 datasets. 69 tissues were utilized perform...
Abstract Background B7-H3, an immune-checkpoint molecule and a transmembrane protein, is overexpressed in non-small cell lung cancer (NSCLC), making it attractive therapeutic target. Here, we aimed to systematically evaluate the value of B7-H3 as target NSCLC via T cells expressing B7-H3-specific chimeric antigen receptors (CARs) bispecific killer engager (BiKE)-redirected natural (NK) cells. Methods We generated CAR B7-H3/CD16 BiKE derived from anti-B7-H3 antibody omburtamab that has been...
Recently, it has been shown that cyclin B1 was degraded mainly before the onset of anaphase in mammalian cells. When a nondegradable form introduced into cells, metaphase-anaphase transition blocked. This blockage not due to failure activating anaphase-promoting complex, nor degradation securin. To resolve question whether this by overexpressing is physiologically relevant or not, we developed novel method estimate relative protein level overexpressed mutant within an individual cell. We...
Cross-links holding on, then letting go: Polyacrylamide main chains were branched with DNA strands, which could be gelatinized by base pairing a thrombin-bound cross-linking strand (see scheme). A complementary can form duplex the to dissolve hydrogel and release thrombin.
We report the development of a very efficient cell-based high throughput screening (HTS) method, which utilizes novel bio-sensor that selectively detects apoptosis based on fluorescence resonance energy transfer (FRET) technique.We generated stable HeLa cell line expressing FRET-based protein. When cells undergo apoptosis, they activate protease called 'caspase-3'. Activation this enzyme will cleave our sensor protein and cause its emission to shift from wavelength 535 nm (green) 486 (blue)....
A hemodynamic Lab-on-a-chip system was developed in this study. This has two unique features: (1) it consists of a microfluidic network with an array endothelial cell seeding sites for testing them under multiple conditions, and (2) the flow rate frequency culture medium microchannel are controlled by pulsation free pump to mimic profile blood vessel different physiological conditions. The investigated conditions were: resting condition normal shear stress 15 dyne cm−2 heart 70 bpm,...
Understanding the survival mechanism of metastatic cancer cells in circulation will provide new perspectives on metastasis prevention and also shed light metastasis-derived drug resistance. In this study, we made it feasible to detect apoptosis circulating tumor (CTCs) real-time by integrating a fluorescence resonance energy transfer (FRET)-based caspase sensor into one vitro microfluidic circulatory system, two vivo models: zebrafish mouse lung model. Our study demonstrated that fluid shear...
Angiogenesis marks the transformation of a benign local tumor into life-threatening disease. Many in vitro assays are available on two-dimensional (2D) platforms, however, limited research has been conducted to investigate behavior tumors and endothelial cells (ECs) grown three-dimensional (3D) platforms. This study provides 3D co-culture spheroid with ECs interplay between cells. In HepG2 hepatocellular carcinoma (HCC) cells, differentiate form tubule networks when co-culture. Addition...
Resistance to doxorubicin (DOX) remains a big challenge breast cancer treatment especially for triple negative (TNBC).Our previous study revealed that the antioxidant system plays an important role in conferring metastasis derived DOX resistance.In this study, we used two-dimensional difference gel electrophoresis (2D-DIGE) proteomics compare expression profiles of two generations TNBC cell lines which have increased metastatic ability nude mice and exhibited resistance DOX.Through careful...
Abstract Circulating tumor cells (CTCs) shed from primary tumors must overcome the cytotoxicity of immune cells, particularly natural killer (NK) to cause metastasis. The microenvironment (TME) protects which is partially executed by cancer‐associated mesenchymal stromal (MSCs). However, mechanisms MSCs influence NK resistance CTCs remain poorly understood. This study demonstrates that enhance cancer in a gap junction‐dependent manner, thereby promoting survival and metastatic seeding...
During apoptosis, cytochrome c is released from mitochondria to the cytosol activate a caspase cascade, which commits cell death process. It has been proposed that release of caused by swelling mitochondrial matrix triggered apoptotic stimuli. To test this theory, we measured directly dynamic re-distribution green fluorescence protein (GFP)-tagged and morphological change within living HeLa cells during u.v.-induced apoptosis. We observed did not swell when was Instead, swelled spherical...
A method was developed for detection of Listeria monocytogenes by polymerase chain reaction amplification followed agarose gel electrophoresis or dot blot analysis with a 32P-labeled internal probe. The technique identified 95 L. strains, 0 12 strains other species, and non-Listeria strains.