A5066227784- Ubiquitin and proteasome pathways
- Pluripotent Stem Cells Research
- Cancer-related Molecular Pathways
- CRISPR and Genetic Engineering
- Microtubule and mitosis dynamics
- Sperm and Testicular Function
- MicroRNA in disease regulation
- Reproductive Biology and Fertility
- Cancer-related molecular mechanisms research
- RNA Interference and Gene Delivery
- Histone Deacetylase Inhibitors Research
- Renal and related cancers
- Mesenchymal stem cell research
- RNA Research and Splicing
- 3D Printing in Biomedical Research
- Circular RNAs in diseases
- Heat shock proteins research
- Hematopoietic Stem Cell Transplantation
- Endoplasmic Reticulum Stress and Disease
- Neuroscience and Neural Engineering
- Phagocytosis and Immune Regulation
- Epigenetics and DNA Methylation
- DNA Repair Mechanisms
- Biochemical and Molecular Research
- Neonatal Respiratory Health Research
Hanyang University
2016-2025
Anyang University
2016-2022
Institute of Cell Biology and Neurobiology
1998-2009
CHA University
2003-2004
University of Pennsylvania
2001
Center for Human Reproduction
1997
Previously, we have reported tissue- and stage-specific expression of miR-372 in human embryonic stem cells so far, not many reports speculate the function this microRNA (miRNA). In study, screened various cancer cell lines including gastric found first time that is expressed only AGS adenocarcinoma line. Inhibition using antisense oligonucleotide (AS-miR-372) suppressed proliferation, arrested cycle at G2/M phase, increased apoptosis cells. Furthermore, AS-miR-372 treatment LATS2, while...
The guinea pig sperm acrosomal matrix is the dense core of acrosome and likely to be important in biogenesis fertilization. Isolated matrices are composed a limited number major bands when analyzed by SDS-polyacrylamide gel electrophoresis, among which M r 67,000 protein that we have termed AM67. Indirect immunofluorescence demonstrated AM67 localized apical segment cauda epididymal acrosome. Immunoelectron microscopy further refined localization M1 (dorsal bulge) domain within Using...
Other SectionsABSTRACTINTRODUCTIONUBIQUITIN-SPECIFIC PROTEASE FAMILYOTUBAIN FAMILYDUB INHIBITORSCONCLUSIONACKNOWLEDGEMENTSFIGURESTABLESREFERENCES
Mouse embryonic stem cells (ESCs) are maintained in serum with leukemia inhibitory factor (LIF) to maintain self-renewal and pluripotency. Recently, a 2i culture method was reported using combination of MEK inhibition (MEKi) GSK3 (GSK3i) LIF ESCs naive ground state. How maintains state remains elusive. Here we show that MEKi GSK3i the ESC by downregulating global DNA methylation through two distinct mechanisms. MEK1 phosphorylates JMJD2C for ubiquitin-mediated protein degradation. Therefore,...
Mammalian target of rapamycin (mTOR) enhances translation from a subset messenger RNAs containing distinct 5′-untranslated region (UTR) sequence features. Here we identify 3′-UTR shortening mRNAs as an additional molecular signature mTOR activation and show that the specific mRNAs. Using genetic or chemical modulations activity in cells mouse tissues, cellular is crucial for shortening. Although long 3′-UTR-containing transcripts minimally contribute to translation, 3-′UTR-shortened...
Previously, we identified the guinea pig sperm acrosomal matrix glycoprotein AM67 and demonstrated that it is most closely related to mouse sp56, initially reported be a cell-surface protein. On contrary, our studies sp56 an intra-acrosomal component. Based upon homology between hypothesized was part of matrix, structure had yet exist in sperm. In this paper, show first appeared late meiotic cells accumulated during spermiogenesis, haploid stage spermatogenic cell development. Using...
Endochondral bone formation begins with the development of a cartilage intermediate that is subsequently replaced by calcified bone. The mechanisms occurring during early chondrogenesis control both mesenchymal cell differentiation into chondrocytes and proliferation are not clearly understood in vertebrates. Indian hedgehog (Ihh), one signaling molecules, known to hypertrophy replacement; these processes particularly important postnatal endochondral rather than chondrogenesis. In this...
Abstract Cisplatin is a chemotherapy drug that causes plethora of DNA lesions and inhibits transcription replication, resulting in the induction apoptosis cancer cells. However, over time, patients develop resistance to cisplatin due repeated treatment thus efficacy limited. Therefore, identifying an alternative therapeutic strategy combining along with targeting factors drive needed. CRISPR/Cas9 system-based genome-wide screening for deubiquitinating enzyme (DUB) subfamily identified USP28...
BACKGROUND: The purpose of this study was to establish the culture conditions required isolate, identify and expand male germ stem cell-like cells (GSC-LC) from testicular tissue patients with non-obstructive azoospermia (NOA). METHODS AND RESULTS: Testicular tissues obtained (two maturation arrest (MA, n = 2) Sertoli cell-only syndrome (SCOS, 11) were dissociated plated into gelatin-coated dishes. After 2–4 weeks, cultures both MA (100%) four SCOS (36.3%) exhibited multicellular colonies,...
The contents of the sperm acrosome are compartmentalized at biochemical and morphological levels. Biochemically, can be considered to comprised two compartments: one consisting readily soluble proteins containing a particulate acrosomal matrix. To test hypothesis that compartmentalization affects release components during course secretion in guinea pig sperm, we examined relationship between presence specific status monitored recovery constituents medium surrounding induced undergo...
Abstract Precise control of gene expression is paramount importance for proper embryonic development. Although a number microRNAs (miRNAs) has been implicated in fine-tuning mRNA translation during development, their exact roles gastrulation, particularly connection with functional targets, have yet to be clarified, regard stage-specific cell migration form three germ layers. We found that miR-124a expressed human stem cells (hESC), but gradually downregulated embryoid body (EB) formation...