OPINION article Front. Microbiol., 10 May 2019Sec. Virology Volume - 2019 | https://doi.org/10.3389/fmicb.2019.01057

African swine fever virus (ASFV) is the etiological agent of a contagious and often lethal disease domestic pigs that has significant economic consequences for industry. The devastating industry in Central Europe East Asia, with current outbreaks caused by circulating strains ASFV derived from 2007 Georgia isolate (ASFV-G), genotype II ASFV. In absence any available vaccines, (ASF) outbreak containment relies on control culling infected animals. Limited cross-protection studies suggest order...

African swine fever virus (ASFV) is the etiological agent of a contagious and often lethal viral disease domestic pigs that has significant economic consequences for industry. The control (ASF) been hampered by unavailability vaccines. Successful experimental vaccines have derived from naturally occurring, cell culture-adapted, or genetically modified live attenuated ASFV. Recombinant viruses harboring engineered deletions specific virulence-associated genes induce solid protection against...

In this study, we analyzed full-length SARS-CoV-2 genomes from multiple countries to determine early trends in the evolutionary dynamics of novel COVID-19 pandemic. Results indicated evolved into at least three phylogenetic groups, characterized by positive selection specific residues accessory proteins ORF3a and ORF8. Also, are reporting potential relevant sites under non-structural nsp6 helicase. Our analysis co-evolution showed evidence epistatic interactions among genome that may be...

The African swine fever virus (ASFV) is currently causing a pandemic affecting wild and domestic from Western Europe to Asia. No commercial vaccines are available prevent (ASF), resulting in overwhelming economic losses the industry. We recently developed recombinant vaccine candidate, ASFVG-ΔI177L, by deleting I177L gene genome of highly virulent ASFV strain Georgia (ASFV-G). ASFV-G-ΔI177L has been proven safe efficacious challenge studies using parental ASFV-G. Here, we present data...

African swine fever virus (ASFV) is causing a devastating pandemic in domestic and wild within an extended geographical area from Central Europe to East Asia, resulting economic losses for the regional industry. There are no commercial vaccines; therefore, disease control relies on identification culling of infected animals. We report here that deletion ASFV gene A137R highly virulent ASFV-Georgia2010 (ASFV-G) isolate induces significant attenuation virulence swine. A recombinant lacking...

The CD2-like African swine fever virus (ASFV) gene 8DR, (also known as EP402R) encodes for a structural transmembrane glycoprotein that has been shown to mediate hemadsorption and be involved in host immunomodulation well the induction of protective immune response. In addition, several natural ASFV isolates showing decreased virulence non-hemadsorbing suggesting an association between altered or deleted forms 8DR attenuation. Here we demonstrate deletion from genome Georgia2010 isolate...

African swine fever virus (ASFV) causes a virulent, deadly infection in wild and domestic is currently causing pandemic covering contiguous geographical area from Central Eastern Europe to Asia. No commercial vaccines are available prevent (ASF), resulting devastating economic losses the industry. The most advanced vaccine candidates live attenuated strains developed using genetically modified virulent parental virus. Recently, we candidate, ASFV-G-ΔI177L, by deleting I177L gene genome of...

African swine fever virus (ASFV) is the etiological agent of (ASF), a disease domestic and wild that has spread throughout large geographical area including Central Europe, East Southeast Asia, Southern Africa. Typically, clinical presentation in affected heavily depends on virulence ASFV strain. Very recently, was detected Dominican Republic (DR) Haiti, constituting first diagnosis more than 40 years Western hemisphere. In this report, pigs inoculated with an field strain isolated from...

African swine fever virus (ASFV) is currently the most dreaded infectious disease affecting global production industry. There no commercial vaccine available, making culling of infected animals current solution to control outbreaks. Effective experimental vaccines have been developed by deleting genes associated with virulence. Deletion ASFV 9GL gene (∆9GL) has resulted in attenuation different strains, although degree varies across isolates. Here, we investigated possibility increased...

No commercial vaccines are available to prevent African swine fever (ASF). The ASF pandemic caused by the virus Georgia 2010 (ASFV-G) strain is seriously affecting pork production in a contiguous geographical area from Central Europe East Asia.

African swine fever virus (ASFV) is the etiological agent of (ASF), a devastating disease affecting domestic and wild currently causing global pandemic, severely production. Here, we demonstrate that deletion previously uncharacterized ASFV gene, H108R from highly virulent ASFV-Georgia2007 (ASFV-G) genome strain, reduces virulence in swine. ASFV-G-ΔH108R, recombinant with gene deleted, was used to evaluate involvement for replication ASFV-G-ΔH108R showed delayed macrophage cultures. A group...

African swine fever virus (ASFV) is causing outbreaks both in domestic pigs and wild boar Europe Asia. In 2018, the largest pig producing country, China, reported its first outbreak of (ASF). Since then, disease has quickly spread to all provinces China other countries southeast Asia, most recently India. Outbreaks occur as far west Poland, one isolated been Belgium. The current strain highly contagious can cause a high degree lethality pigs, leading widespread costly losses industry....

African swine fever virus (ASFV) causes a contagious and frequently lethal disease of pigs with significant economic consequences to the industry. The ASFV genome encodes for more than 160 genes, but only few them have been studied in detail. Here we report characterization open reading frame (ORF) MGF360-16R. Kinetic studies RNA transcription demonstrated that MGF360-16R gene is transcribed as late protein. Analysis host-protein interactions using yeast two-hybrid screen identified SERTA...

African swine fever virus (ASFV) causes a devastating disease of that has caused outbreaks in Central Europe since 2007, spreading into Asia 2018. ASFV is large, structurally complex with large dsDNA genome encoding for more than 160 genes, most them still uncharacterized. p22, encoded by the gene KP177R, an early transcribed, structural protein located particle. Although its exact function unknown, p22 recently been identified as interacting partner several host proteins. Here, we describe...

African swine fever virus (ASFV) is the etiological agent of a frequently lethal disease, ASF, affecting domestic and wild swine. Currently, ASF causing pandemic pig production in Eurasia. There are no vaccines available, therefore control disease based on culling infected animals. We report here that deletion ASFV gene A104R, histone-like protein, from genome highly virulent ASFV-Georgia2010 (ASFV-G) strain induces clear decrease virulence when experimentally inoculated A recombinant...

African swine fever virus (ASFV) is responsible for an ongoing pandemic that affecting central Europe, Asia, and recently the Dominican Republic, first report of disease in Western Hemisphere over 40 years. ASFV a large, complex with double-stranded DNA (dsDNA) genome carries more than 150 genes, most which have not been studied. Here, we assessed role MGF110-5L-6L gene during replication cell cultures experimental infection swine. A recombinant deleted (ASFV-G-ΔMGF110-5L-6L) was developed...

The African swine fever virus (ASFV) mutant ASFV-G-∆I177L is a safe and efficacious vaccine which induces protection against the challenge of its parental virus, Georgia 2010 isolate. Although genetic DIVA (differentiation between infected vaccinated animals) assay has been developed for this vaccine, still there not serological test differentiating animals with those wild-type viruses. In report, we describe development having deleted EP402R gene, encodes viral protein responsible mediating...

ABSTRACT Two near full-length sequences of vesicular stomatitis Indiana virus (VSIV), representing endemic VSIV lineages circulating in cattle Mexico, are reported. These will allow us to gain more insight into the genetic relationship between viruses Mexico and emergence epizootic United States.

Vesicular stomatitis virus (VSV) causes sporadic outbreaks of vesicular disease in the southwestern United States. The intrinsic characteristics epidemic strains associated with these are poorly understood. In this study, we report distinctive genomic and biological an (NJ0612NME6) strain VSV compared endemic (NJ0806VCB) strain. Genomic comparisons between two revealed a total 111 nucleotide differences (23 non-synonymous) potentially relevant replacements located P, G, L proteins. When...

African swine fever (ASF) is currently causing an epizootic, affecting pigs throughout Eurasia, and significant economic losses in the industry. ASF caused by virus (ASFV) that consists of a large dsDNA genome encodes for more than 160 genes; few these genes have been studied detail. ASFV contains four multi-gene family (MGF) groups implicated regulating immune response host specificity; however, individual roles most not well studied. Here, we describe evaluation previously uncharacterized...

African swine fever (ASF) is an important disease in currently producing a pandemic affecting pig production worldwide. Except Vietnam, where two vaccines were recently approved for controlled use the field, no vaccine commercially available control. Up to now, most effective developed are based on of live-attenuated viruses. Most these promising candidates by deleting virus genes involved process viral pathogenesis and production. Therefore, via genomic modification parental field strains,...