A5090998204- Mesenchymal stem cell research
- 3D Printing in Biomedical Research
- Corneal Surgery and Treatments
- Tissue Engineering and Regenerative Medicine
- Pluripotent Stem Cells Research
- Bone Tissue Engineering Materials
- Osteoarthritis Treatment and Mechanisms
- Ocular Surface and Contact Lens
- Electrospun Nanofibers in Biomedical Applications
- dental development and anomalies
- Corneal surgery and disorders
- COVID-19 Clinical Research Studies
- Neurogenesis and neuroplasticity mechanisms
- Biomedical Ethics and Regulation
- Neonatal Respiratory Health Research
- Neuroscience and Neural Engineering
- Dental Implant Techniques and Outcomes
- Cleft Lip and Palate Research
- Oral and Maxillofacial Pathology
- Animal Genetics and Reproduction
- Renal and related cancers
- SARS-CoV-2 and COVID-19 Research
- Tendon Structure and Treatment
- Congenital heart defects research
- Knee injuries and reconstruction techniques
Universidade Federal de São Paulo
2009-2020
Instituto Butantan
2008-2014
Fundação de Apoio à Universidade Federal de São Paulo
2008
Purpose.: To determine the outcome of use a tissue-engineered cell sheet composed human undifferentiated immature dental pulp stem cells (hIDPSC) for ocular surface reconstruction in an animal model total limbal deficiency (LSCD). Methods.: LSCD was induced by application 0.5 M NaOH to right eye rabbits 25 seconds (mild chemical burn [MCB]) and 45 (severe [SCB]). After 1 month, superficial keratectomy performed remove fibrovascular pannus that covered animals' burned corneas. A hIDPSC...
Dental pulp (DP) can be extracted from child's primary teeth (deciduous), whose loss occurs spontaneously by about 5 to 12 years. Thus, DP presents an easy accessible source of stem cells without ethical concerns. Substantial quantities excellent quality and at early (2–5) passages are necessary for clinical use, which currently is a problem use adult cells. Herein, DPs were cultured generating least during six months through multiple mechanical transfers into new culture dish every 3–4...
Limbal stem cells (LSC) are self-renewing, highly proliferative in vitro, which express a set of specific markers and vivo have the capacity to reconstruct entire corneal epithelium cases ocular surface injury. Currently, LSC transplantation is commonly used procedure patients with either uni- or bilateral total limbal deficiency (TLSCD). Although holds great promise for patients, several problems need be overcome. In order find an alternative source that can partially substitute cornea...
Induced pluripotent stem cells (iPSCs) can be created by forcing expression of certain genes in fibroblasts or other somatic cell types, reversing them to a state similar that embryonic (ESC). Here, we used human immature dental pulp (hIDPSCs) as an alternative source for creating iPSC. hIDPSCs easily isolated from accessible tissue young and adult patients. possess fibroblast-like morphology, retaining characteristics multipotent cells. Reprogramming was fast, producing primary hIDPSC-iPSC...
During fetal development, mesenchymal progenitor (MP) cells are co-localized in major hematopoietic territories, such as yolk sac (YS), bone marrow (BM), liver (LV), and others. Studies using mouse human MP isolated from fetus have shown that these very similar but not identical to adult stem (MSC). Their differentiation potential is usually restricted production of highly committed osteogenic chondrogenic precursors. Such properties can be useful for tissue regeneration, when a great number...
Resorbable synthetic and natural polymer-based membranes have been extensively studied for guided tissue regeneration. Alloplastic biomaterials are often used regeneration due to their lower immunoreactivity when compared with allogeneic xenogeneic materials. Plenum® Guide is a membrane material based on polydioxanone (PDO), whose surface morphology closely mimics the extracellular matrix. In this study, was collagen as barrier bone-tissue in terms of acute subchronic systemic toxicity....
Innumerous protocols, using the mouse embryonic stem (ES) cells as model for in vitro study of neurons functional properties and features, have been developed. Most these protocols are short lasting, which, therefore, does not allow a careful analysis maturation, aging, death processes. We describe here novel efficient long-lasting protocol ES differentiation into neuronal cells. It consists obtaining embryoid bodies, followed by induction with retinoic acid nonadherent bodies...
ABSTRACT Objective: To describe the first series of cases autologous chondrocyte implantation (ACI) in collagen membrane performed Brazil. Methods: ACI was 12 knees 11 patients, aged 32.1 ± 10.9 years, with 5.3 2.6 cm2 full-thickness knee cartilage lesions, a six-month minimum follow-up. Two surgical procedures were performed: arthroscopic biopsy for isolation and expansion chondrocytes, which seeded onto implanted lesion site; characterization cultured cells implant using immunofluorescence...
<title>Abstract</title> Treatment of complex craniofacial deformities is still a challenge for medicine and dentistry because few approach therapies are available on the market that allows rehabilitation using 3D-printed medical devices. Thus, this preliminary study aims to evaluate bioactivity β-tricalcium phosphate (β-TCP) scaffolds polydioxanone membrane (PDO) with cell-based (ASCs) tissue engineering guided bone regeneration in critical calvary defects rats. Methods: Male rats were...
The COVID-19 pandemic has already reaped thousands of lives, although many scientific studies showed the possibility this scenario. Currently, further attention is provided to patients depicting comorbidities such as respiratory or immunocompromised diseases, hypertension, and diabetes, these individuals show a worse prognosis. Cell therapies using stem cells and/or defense cells, combined not with traditional treatment, could be an outstanding strategy for management since treatments can...