Abstract A molecular epidemiological study on common diarrheal viruses was conducted in Ho Chi Minh City, Vietnam between October 2002 and September 2003. Fecal samples were collected from 1,010 hospitalized children with acute gastroenteritis. Those screened for groups A, B, C rotavirus, adenovirus, genogroups I II norovirus (NoV), sapovirus (SaV), human astrovirus (HAstV) by RT‐multiplex PCR, the positive specimens characterized further ELISA, nested or sequencing. Among detected, group...

A total of 82 fecal specimens which were known to be negative for rotavirus, adenovirus, norovirus, sapovirus, and astrovirus collected from infants children with acute gastroenteritis in Chiang Mai, Thailand, January December 2005 screened human parechovirus (HPeV). HPeV was detected by reverse transcription-PCR a primer pair that amplified the 5' untranslated region its genome genotyped sequencing VP1 region. 12 tested, detection rate found 14.6%. The capsid gene successfully sequenced...

Delayed wound healing reduces the quality of life (QOL) patients. Thus, understanding mechanism is indispensable for better management. However, role innate immunity in thus far unknown. Recently involvement TLR3 has been evaluated. The systemic administration polyriboinosinic-polyribocytidylic acid (poly I:C ; a substitute viral dsRNA and ligand toll-like receptor 3), enhances vivo. aim this study to improve our link between human healing, particularly re-epithelialization. present showed...

Viral gastroenteritis is a significant global health concern. An effective, rapid, and easy-to-use diagnostic tool essential for screening causative viruses. Forty-eight samples, known to be infected with one of the following viruses: norovirus, group A rotavirus, astrovirus, adenovirus, sapovirus determined by reverse transcription-PCR nucleotide sequencing, were evaluated Fast Track Diagnostics (FTD) viral assay. The assay demonstrated 100% specificity all viruses matched RT-PCR...

Rubella infection (RuV) during early pregnancy is a known cause of congenital rubella syndrome (CRS). However, the mechanisms by which virus crosses placenta and infects fetus are not fully understood. It has been that various kinds cell stresses can occur formation. Previously, we demonstrated low-glucose-induced endoplasmic reticulum stress could drastically enhance RuV in immortalized human first-trimester trophoblast cells. In this study, investigated roles oxidative these Oxidative was...

Of 362 fecal specimens collected from infants and children hospitalized with acute gastroenteritis in Sri Lanka September 2005 to August 2006, 30 (8.3%) were positive for human parechovirus (HPeV). Six different HPeV genotypes, including HPeV1, -3, -4, -5, -10, -11, identified, of these, HPeV11 was reported the first time.

Problem The functions of vaginal lactobacilli in susceptibility to infectious diseases as regards epithelial barrier integrity and wound healing remain incompletely understood. Method study Lactobacillus crispatus , one the most common species vagina among protective against sexually transmitted infections, was cocultured with an immortalized human cell line ( MS 74), a scratch assay performed evaluate re‐epithelialization. concentration vascular endothelial growth factor A VEGF ) measured...

SUMMARY Information about viral acute respiratory infections (ARIs) is essential for prevention, diagnosis and treatment, but it limited in tropical developing countries. This study described the clinical epidemiological characteristics of ARIs children hospitalized Vietnam. Nasopharyngeal samples were collected from with at Ho Chi Minh City Children's Hospital 2 between April 2010 May 2011 order to detect viruses by polymerase chain reaction. Viruses found 64% 1082 patients, 12% being...

Abstract Nucleotide and amino acid sequences of the VP8 ☆ gene five Vietnamese P[6] rotavirus strains detected from hospitalized patients with acute gastroenteritis were analyzed compared other human porcine rotaviruses. It is interest that these had greatest identity two Italian strains, 134/04‐10 134/04‐11. To our knowledge, rotaviruses are rare belonging to lineage I cluster into sublineage Ic rotaviruses, not Ia, as have done so far. Sequence analysis VP7 was also performed. The results...

Of 362 fecal samples collected from children with acute gastroenteritis in Sri Lanka during 2005-2006, 30 (8.3%) were positive for human parechovirus (HPeV) by reverse transcription-PCR. A novel HPeV, designated as HPeV10, was identified 2 sequence analysis of the viral protein 1 gene detected HPeVs.

SUMMARY Molecular epidemiology and clinical impact of human rhinovirus (HRV) are not well documented in tropical regions. This study compared the characteristics HRV to other common viral infections investigated molecular hospitalized children with acute respiratory (ARIs) Vietnam. From April 2010 May 2011, 1082 nasopharyngeal swabs were screened for viruses by PCR. VP4/VP2 sequences further characterized. was most commonly detected virus (30%), which 70% diagnosed as either pneumonia or...

Abstract Over the last decade, rotavirus G1 has represented most common genotype worldwide. Since 2000, prevalence of decreased in some countries such as Japan and China. To monitor trend VP7 encoding gene G1, we performed a sequence analysis 74 strains isolated Japan, China, Thailand, Vietnam during period from 2002 to 2005. The phylogenetic tree showed that all studied four clustered into lineage III, same majority China 1990 1991. Examination deduced amino acid sequences revealed an...

Abstract A total of 329 fecal specimens, which had been known to be negative for rotavirus, adenovirus, norovirus, sapovirus, and astrovirus, were collected from infants children with acute gastroenteritis in Japan Thailand during 2005–2008 screened human bocavirus (HBoV). HBoV was detected by PCR a primer pair that amplified the NP1 region its genome genotyped sequencing VP1/VP2 region. Of samples tested, 6 (1.8%) positive HBoV. these, five one sample Thailand, detection rates each country...

One hundred and twenty five fecal specimens were collected from sporadic cases of acute gastroenteritis in a pediatric clinic Osaka City, Japan July 2004 to June 2005 tested for the presence rotavirus, norovirus, sapovirus, astrovirus, adenovirus by RT-multiplex PCR. Among diarrheal viruses detected, norovirus was most prevalent (19.2%, 24 125), followed group A rotavirus (18.4%, 23 astrovirus (1.6%, 2 (0.8%, 1 respectively. Interestingly, sapovirus infection identified with high incidence...

A total of 29 Campylobacter jejuni and C. coli strains were isolated from Thai Japanese children with diarrhea using the Loop-mediated Isothermal Amplification method. The samples evaluated for mutations in gyrA 23S rRNA order to assess resistance against fluoroquinolones macrolides, respectively. Among strains, 9 (8 1 coli) children, other 20 (C. jejuni) children. High fluoroquinolone rates observed (66.7%) (90%) Macrolide was not but at a considerable rate 12.5% cohort. results indicate...

Human astrovirus (HAstV) infection is one of the leading causes acute gastroenteritis in young children. The present study reports outbreak HAstV children with Kyoto, Japan, during COVID-19 pandemic, 2021.A total 61 stool samples were collected from who visited a pediatric outpatient clinic Maizuru city, Japan July to October, 2021. was screened by RT-PCR, and genotypes identified nucleotide sequence analysis.Of cases gastroenteritis, 20 mono-infected alone. In addition, mixed NoV, RVA also...

Abstract Sequence analysis of the capsid gene Aichi viruses was performed on 12 strains detected in Japan, Bangladesh, Thailand, and Vietnam during 2002–2005. The phylogenetic tree constructed from 17 nucleotide sequences studied reference demonstrated that virus clustered into two branches. A classification based proposed, which lineage I consists Vietnam, Germany, II includes Bangladeshi a Brazilian strain. J. Med. Virol. 80: 1222–1227, 2008. © 2008 Wiley‐Liss, Inc.

Problem Epidemiological data suggested that pandemic influenza increased the risks of spontaneous abortion and premature labor, while seasonal also risk schizophrenia in adolescence. However, their pathogenesis is so far unknown. Method study The first trimester trophoblast cell lines, namely, Swan71 HTR8 cells were challenged with A/Udorn/72 virus (H3N2). At indicated time points, examined for expression proteins. Viral replication culture media, apoptosis human leukocyte antigen (HLA)‐G...

Sequence and phylogenetic analyses of the rotavirus VP7 gene were performed on 52 human G2 G4 strains isolated in Japan, China, Thailand, Vietnam during 2001-2003. All genotype included study clustered into lineage II tree, together with majority global detected since 1995. The amino acid substitution at position 96 from aspartic to asparagine was noted among emerging or re-emerging 2002-2003. Genotype grouped Ia whereas Japanese Ic which Argentina, Italy, Paraguay, Uruguay. It is noteworthy...