A5052417296- Polyamine Metabolism and Applications
- Cannabis and Cannabinoid Research
- Amino Acid Enzymes and Metabolism
- melanin and skin pigmentation
- Mast cells and histamine
- Neurotransmitter Receptor Influence on Behavior
- Cutaneous Melanoma Detection and Management
- Click Chemistry and Applications
- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- Cellular Mechanics and Interactions
- Hippo pathway signaling and YAP/TAZ
- Electrolyte and hormonal disorders
- Food Allergy and Anaphylaxis Research
- Diet, Metabolism, and Disease
- Skin Protection and Aging
- Drug Transport and Resistance Mechanisms
- Neuroscience of respiration and sleep
- Biochemical Analysis and Sensing Techniques
- Neurobiology and Insect Physiology Research
- Olfactory and Sensory Function Studies
- CRISPR and Genetic Engineering
- Cell Adhesion Molecules Research
Universidad de Murcia
2014-2024
Instituto Murciano de Investigación Biosanitaria
2014-2024
Cutaneous melanoma, a lethal skin cancer, arises from malignant transformation of melanocytes. Solar ultraviolet radiation (UVR) is major environmental risk factor for melanoma since its interaction with the generates DNA damage, either directly or indirectly via oxidative stress. Pheomelanin pigments exacerbate stress in melanocytes by UVR-dependent and independent mechanisms. Thus, considered to contribute melanomagenesis, particularly people pheomelanic pigmentation. The melanocortin 1...
Abstract Metabolic dysfunction‐associated steatotic liver disease (MASLD) is a chronic condition that often progresses to more advanced stages, such as metabolic steatohepatitis (MASH). MASH characterized by inflammation and hepatocellular ballooning, in addition hepatic steatosis. Despite the relatively high incidence of population its potential detrimental effects on human health, this still not fully understood from pathophysiological perspective. Deregulation polyamine levels has been...
Mahogunin Ring Finger 1 is an E3-ubiquitin ligase encoded by the color gene MGRN1. Our previous in vitro and vivo studies demonstrated that Mgrn1 deletion mouse melanoma cells induced cell differentiation adhesion, decreased motility invasion on collagen I, lung colonization model. Here, we investigated role of MGRN1 human morphology, adhesion expression genes/proteins involved EMT-like transition. We wild-type BRAF adopted a clustering-like morphology with permanent abrogation resulting...
The intracellular levels of polyamines, polycations implicated in proliferation, differentiation and cell survival, are regulated by controlling their biosynthesis, catabolism transport. Antizymes antizyme inhibitors key regulatory proteins polyamine affecting ornithine decarboxylase, the rate-limiting biosynthetic enzyme, uptake. We recently described molecular function a novel inhibitor (AZIN2). However, physiological AZIN2 mammals is mostly unknown. To gain insight on tissue expression...
We examined in detail the distribution of AZIN2 (antizyme inhibitor 2) expression adult mouse hindbrain and neighboring spinal cord. AZIN2, similar to previously known AZIN1, is a recently‐discovered, functional paralog ornithine decarboxylase (ODC). Due their structural similarity ODC, both AZIN1 counteract inhibitory action 3 antizymes (AZ1‐3) on ODC synthesis polyamines, thus increasing intracytoplasmic levels polyamines. strongly, but heterogeneously, expressed brain. Our study uses line...
The specific role of polyamines in the testis physiology is not fully understood. Antizymes (OAZs) and antizyme inhibitors (AZINs) are modulators ornithine decarboxylase (ODC), a key enzyme polyamine biosynthesis uptake. Although three known OAZs expressed testis, only OAZ3 has been proven to have an essential male fertility. Regarding two existing AZINs, AZIN2 most abundantly member this gonad. Whereas previous studies suggested that might participate mouse spermatogenesis,...
Ornithine decarboxylase (ODC) is the key enzyme in polyamine biosynthetic pathway. ODC levels are controlled by polyamines through induction of antizymes (AZs), small proteins that inhibit and target it to proteasomal degradation without ubiquitination. Antizyme inhibitors (AZIN1 AZIN2) homologous bind AZs counteract their negative effect on ODC. Whereas AZIN1 well‐characterized proteins, little known structure stability AZIN2, lastly discovered member this regulatory circuit. In work we...
Common variants of the MC1R gene coding α-melanocyte stimulating hormone receptor are associated with light skin, poor tanning, blond or red hair, and increased melanoma risk, due to pigment-dependent -independent effects. This complex phenotype is usually attributed impaired activation cAMP signaling. However, several show significant residual coupling efficiently activate mitogenic extracellular signal-regulated kinase 1 2 (ERK1/2) Yet, signaling key actions wildtype variant have never...
Mahogunin Ring Finger 1 (MGRN1) is an E3-ubiquitin ligase absent in dark-furred mahoganoid mice. We investigated the mechanisms of hyperpigmentation Mgrn1-null melan-md1 melanocytes, Mgrn1-KO cells obtained by CRISPR-Cas9-mediated knockdown Mgrn1 melan-a6 and depleted MGRN1 siRNA treatment. Mgrn1-deficient melanocytes showed higher melanin content associated with increased melanosome abundance fraction melanosomes highly melanized maturation stages III-IV. Expression, post-translational...
Background: Alterations in the neural polyamine system are known to be associated with different brain pathological conditions. In addition, regulation of enzymes involved metabolism such as ornithine decarboxylase (ODC), antizymes (AZs), and antizyme inhibitors (AZINs) is critical during development. However, while most studies focus on ODC AZs, less about AZIN expression function brain. Thus, our aim was analyze pattern AZIN2 postnatal development, its distribution, possible implication...
The role that the induction of cardiac ornithine decarboxylase (ODC), a key enzyme in polyamine biosynthesis, by beta-adrenergic agents may have heart hypertrophy is controversial issue. Besides, signaling pathways related to ODC regulation not been fully elucidated. Here we show Balb C mice stimulation activity adrenergic was mainly mediated β2 -adrenergic receptors, and this lower hypertrophic heart. Interestingly, abolished L-calcium channel antagonists verapamil nifedipine. In addition,...
The administration of l-arginine hydrochloride has been used for testing pituitary secretion in humans, and as an experimental model induction acute pancreatitis rats mice. Whereas the first case, amino acid is associated with hiperkalemia, no data are available on possible changes potassium homeostasis. present study shows that to mice or other cationic acids almost duplicate plasma levels. This effect was a marked decrease tissue both pancreas liver. No were found tissues. These cannot be...
Mahogunin Ring Finger 1 (MGRN1), a ubiquitin ligase expressed in melanocytes, interacts with the α melanocyte-stimulating hormone receptor, well-known melanoma susceptibility gene. Previous studies showed that MGRN1 modulates phenotype of mouse melanocytes and cells, effects on pigmentation, shape, motility. Moreover, knockdown augmented burden DNA breaks indicating loss promoted genomic instability. However, data concerning roles human cells remain scarce. We analyzed cells. Transient...
Ornithine decarboxylase (ODC) is a key enzyme in the biosynthesis of polyamines, organic cations that are implicated many cellular processes. The regulated at post-translational level by an unusual system includes antizymes (AZs) and antizyme inhibitors (AZINs). Most studies on this complex regulatory mechanism have been focused human rodent cells, showing AZINs (AZIN1 AZIN2) homologues ODC but devoid enzymatic activity. Little known about Xenopus its paralogues, spite relevance as model...
Abstract Ornithine decarboxylase (ODC) is a key enzyme in the biosynthesis of polyamines, organic cations that are implicated many cellular processes. The regulated at post-translational level by an unusual system includes antizymes (AZs) and antizyme inhibitors (AZINs). Most studies on this complex regulatory mechanism have been focused human rodent cells, showing AZINs (AZIN1 AZIN2) homologues ODC but devoid enzymatic activity. Little known about Xenopus its paralogues, spite relevance as...