A5079054554- Immune responses and vaccinations
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Tuberculosis Research and Epidemiology
- Immune Response and Inflammation
- Adipose Tissue and Metabolism
- Neuroinflammation and Neurodegeneration Mechanisms
- Calcium signaling and nucleotide metabolism
- Immune cells in cancer
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Cytomegalovirus and herpesvirus research
- Cytokine Signaling Pathways and Interactions
- Immune Cell Function and Interaction
- Cardiovascular Disease and Adiposity
- Sepsis Diagnosis and Treatment
- Adipokines, Inflammation, and Metabolic Diseases
Universidade Federal de Santa Catarina
2018-2023
COVID-19 has accounted for more than 6 million deaths worldwide. Bacillus Calmette-Guérin (BCG), the existing tuberculosis vaccine, is known to induce heterologous effects over other infections due trained immunity and been proposed be a potential strategy against SARS-CoV-2 infection. In this report, we constructed recombinant BCG (rBCG) expressing domains of nucleocapsid spike proteins (termed rBCG-ChD6), recognized as major candidates vaccine development. We investigated whether rBCG-ChD6...
Abstract Background and Purpose Neutrophil overstimulation plays a crucial role in tissue damage during severe infections. Because pathogen‐derived neuraminidase (NEU) stimulates neutrophils, we investigated whether host NEU can be targeted to regulate the neutrophil dysregulation observed Experimental Approach The effects of inhibitors on lipopolysaccharide (LPS)‐stimulated neutrophils from healthy donors or COVID‐19 patients were determined by evaluating shedding surface sialic acids, cell...
Abstract Retinoic acid (RA) is a fundamental vitamin A metabolite involved in regulating immune responses through the nuclear RA receptor (RAR) and retinoid X receptor. While performing experiments using THP-1 cells as model for Mycobacterium tuberculosis infection, we observed that serum-supplemented cultures displayed high levels of baseline RAR activation presence live, but not heat-killed, bacteria, suggesting M. robustly induces endogenous pathway. Using vitro vivo models, have further...
Neutrophil overstimulation plays a crucial role in tissue damage during severe infections. Neuraminidase (NEU)-mediated cleavage of surface sialic acid has been demonstrated to regulate leukocyte responses. Here, we report that antiviral NEU inhibitors constrain host activity, release, ROS production, and NETs released by microbial-activated human neutrophils. In vivo, treatment with Oseltamivir results infection control survival peritonitis pneumonia models sepsis. Single-cell RNA...
Abstract Monocyte counts are increased during human tuberculosis (TB) but it has not been determined whether Mycobacterium ( Mtb ) directly regulates myeloid commitment. We demonstrated that exposure to directs primary CD34 + cells differentiate into monocytes/macrophages. In vitro conversion did require type I or II IFN signaling. contrast, enhanced IL-6 responses by cell cultures and IL-6R neutralization inhibited differentiation decreased mycobacterial growth in vitro. Integrated systems...
Abstract Tissue-resident macrophages comprise the most abundant immune cell population in healthy adipose tissue. Adipose tissue macrophage populations change during metabolic stress and ageing, are thought to contribute pathogenesis of obesity. Here, we studied subpopulations steady state, response nutritional infectious challenges. Using comprehensive cell-surface-based gene expression analyses, found that tissue-resident from epididymal white (eWAT) tightly associate with blood vessels,...