A5057244234- Peroxisome Proliferator-Activated Receptors
- Cancer, Lipids, and Metabolism
- DNA Repair Mechanisms
- Metabolomics and Mass Spectrometry Studies
- Cancer, Hypoxia, and Metabolism
- Fatty Acid Research and Health
- Microtubule and mitosis dynamics
- Mitochondrial Function and Pathology
- Metabolism, Diabetes, and Cancer
- Ion channel regulation and function
- Genomics and Chromatin Dynamics
- Drug Transport and Resistance Mechanisms
- Phosphodiesterase function and regulation
- Metabolism and Genetic Disorders
- Cardiac electrophysiology and arrhythmias
- Epigenetics and DNA Methylation
- Lipid metabolism and biosynthesis
- Cholesterol and Lipid Metabolism
- Cancer-related Molecular Pathways
- Mast cells and histamine
- Autophagy in Disease and Therapy
- Ion Channels and Receptors
- RNA Research and Splicing
- Cardiac Arrhythmias and Treatments
- Adipose Tissue and Metabolism
Foie, Métabolisme, Cancer
2015-2021
Inserm
2012-2021
Université de Tours
2012-2021
Centre Hospitalier Universitaire de Tours
2019
Centre Hospitalier Universitaire d'Angers
2019
Centre de recherche Translationnelle en Médecine moléculaire
2019
Centre Hospitalier Universitaire de Grenoble
2016
Clinical Investigation Center Plurithematic Tours
2016
Pfizer (United States)
2000-2008
Pfizer (France)
2002-2007
Using immunodepletion of cyclin E and the inhibitor protein p21WAF/CIP1, we demonstrate that protein, in association with Cdk2, is required for elongation phase replication on single-stranded substrates. Although E/Cdk2 likely to be major target by which p21 inhibits initiation sperm DNA replication, can inhibit through a mechanism dependent PCNA. While complex appears have role another Cdk kinase, possibly A/Cdk, may involved later step controlling switch from elongation. The provision...
The degradation of the extracellular matrix by cancer cells represents an essential step in metastatic progression and this is performed cell structures called invadopodia. NaV1.5 (also known as SCN5A) Na(+) channels are overexpressed breast tumours associated with occurrence. It has been previously shown that activity enhances invasiveness through perimembrane acidification subsequent cysteine cathepsins. Here, we show colocalises Na(+)/H(+) exchanger type 1 (NHE-1) caveolin-1 at sites...
NaV1.5 voltage-gated sodium channels are abnormally expressed in breast tumours and their expression level is associated with metastatic occurrence patients' death. In cancer cells, activity promotes the proteolytic degradation of extracellular matrix enhances cell invasiveness. this study, we showed that extinction human cells almost completely abrogated lung colonisation immunodepressed mice (NMRI nude). Furthermore, demonstrated ranolazine (50 μM) inhibited currents reduced NaV1.5-related...
The replication licensing factor (RLF) is an essential initiation that involved in preventing re-replication of chromosomal DNA a single cell cycle. In Xenopus egg extracts, it can be separated into two components: RLF-M, complex MCM/P1 polypeptides, and RLF-B, which currently unpurified. this paper we investigate variations RLF activity throughout the Total low metaphase, due to lack RLF-B presence inhibitor. rapidly activated on exit from then declines during interphase. inhibitor present...
ABSTRACT Extracts of activated Xenopus eggs in which protein synthesis has been inhibited support a single round chromosomal DNA replication. Affinity-depletion cyclin dependent kinases (Cdks) from these extracts blocks the initiation We define ‘S-phase promoting factor’ (SPF) as Cdk activity required for replication Cdk-depleted extracts. Recombinant cyclins A and E, but not B, showed significant SPF activity. High concentrations promoted entry into mitosis, In contrast, high E1 neither...
Adenosine 5′-triphosphate (ATP) is found in high concentrations the extracellular microenvironment of tumours and postulated to play critical roles cancer progression. In present study, we that stimulation human MCF-7 breast cells with 30 µM ATP increased their migration by 140±31%, whereas it had minor or no effect on proliferation. This was prevented ectonucleotidase apyrase antagonized suramin pyridoxalphosphate-6-azophenyl-2′,4′-disulfonic acid, consistently participation P2 receptors....
Abstract The development of metastases largely relies on the capacity cancer cells to invade extracellular matrices (ECM) using two invasion modes termed ‘mesenchymal’ and ‘amoeboid’, with possible transitions between these modes. Here we show that SCN4B gene, encoding for β4 protein, initially characterized as an auxiliary subunit voltage-gated sodium channels (Na V ) in excitable tissues, is expressed normal epithelial reduced protein levels breast biopsies correlate high-grade primary...
Abstract Loss of epithelial polarity and gain in invasiveness by carcinoma cells are critical events the aggressive progression cancers depend on phenotypic transition programs such as epithelial-to-mesenchymal (EMT). Many studies have reported aberrant expression voltage-gated sodium channels (Na V ) carcinomas specifically Na 1.5 isoform, encoded SCN5A gene, breast cancer. activity, through an entry ions, cancer is associated with increased invasiveness, but its participation to EMT has be...
// Audrey Gambade 1, * , Sami Zreika 2, Maxime Guéguinou 3 Igor Chourpa 4 Gaëlle Fromont 3, 5 Ana Maria Bouchet Julien Burlaud-Gaillard Marie Potier-Cartereau Sébastien Roger 1 Vincent Aucagne 6 Stéphan Chevalier Christophe Vandier Caroline Goupille 5, Günther Weber 4, Inserm, UMR1069, Nutrition, Croissance et Cancer, Tours, France 2 Department of Medical Lab Technology, Jinan University, Tripoli, Lebanon Ion channel network Canceropole Grand Ouest Université François Rabelais, CHRU Hôpital...
The P2X7 receptor is an ATP-gated cation channel with a still ambiguous role in cancer progression, proposed to be either pro- or anti-cancerous, depending on the cell type tumour. Its mammary progression not yet defined. Here, we show that functional highly aggressive cells, and induces change morphology fast F-actin reorganization formation of filopodia, promotes invasiveness through both 2- 3-dimensional extracellular matrices vitro. Furthermore, sustains Cdc42 activity acquisition...