Nitrous oxide (N2O) is emitted from pig manure composting, and the emission correlates with nitrite (NO2-) accumulation in composting material. In present study, we added nitrite-oxidizing bacteria (NOB)to inhibit NO2- evaluated its effect on N2O a laboratory-scale experiment. Mature compost (MPMC) containing NOB at 10(6) MPN g(-1) WM or cultured MPMC (cul-MPMC) 10(11) was after thermophilic phase of composting. The addition these materials prevented accumulation, promoting oxidation to...

Abstract A full-scale swine-wastewater activated sludge treatment plant that contains naturally enriched anammox biofilms was investigated for 2 years. Red biofilm in this system included Planctomycetes at a maximum of 62.5% the total bacteria diversity, including Candidatus Jettenia and Brocadia. The operated with an influent containing 1,104 ± 513 mg/L biochemical oxygen demand (BOD) 629 198 nitrogen (TN) (BOD/N 1.78 0.58) volumetric BOD loading rate 0.32 0.12 kg/m3/d. Notwithstanding...

A thermophilic bacterium, strain TAT105, was isolated from compost made of animal wastes. TAT105 had high tolerance to ammonium nitrogen up 1200 mM, and highly assimilated during the growth on swine feces. The classified into Bacillus, close Bacillus pallidus. To evaluate effect adding ammonia (NH3) emission composting process wastes, laboratory scale done. NH3 tended be lower loss smaller in TAT105-added material than control which not added. Thermophilic ammonium-tolerant bacteria...

Abstract The present study investigated the nitrogen balance in swine manure composting to evaluate effect of nitrite (NO− 2) accumulation, which induces nitrogenous emissions, such as N2O, during compost maturation. During active composting, most N losses result from NH3 emission, was 9.5% initial total (TNinitial), after which, NO− 2 began accumulate only ammonia-oxidizing bacteria proliferated. After addition mature (MSC), including nitrite-oxidizing (NOB), could prevent accumulation and...

Bacillus sp. strain TAT105 is a thermophilic, ammonium-tolerant bacterium that grows assimilating ammonium nitrogen and reduces ammonia emission during composting of swine feces. To develop practical use TAT105, dried solid culture (5.3 × 10(9) CFU/g dry matter) was prepared as an additive. It could be stored for one year without significant reduction TAT105. Laboratory-scale feces conducted by mixing the When additive, mixed with equal weight water day before use, added to obtain...

The nitrogen concentrations of piggery wastewater and its activated sludge treatment effluent were surveyed in nine farms with various facilities. biological oxygen demand suspended solids the sufficiently removed by because their averaged 59 mg·L-1 45 mg·L-1, respectively; however, was not concentration 430 mg·L-1. When higher than general permissible limit under Japanese law, composition tended to be mainly ammonium when pH 8 or higher, a mixture ammonium, nitrite and/or nitrate below 8....

This study aimed to characterize microbial community dynamics in aerated cow manure slurry at different aeration intensities.Batch aerobic treatments were set up 5-l jar fermentor, each containing 3 l of slurry; the slurries subjected low, medium and high (50, 150 250 ml min(-1), respectively) for 9 days. Microbial composition was determined using terminal restriction fragment length polymorphism a clone library targeting 16S rRNA genes. High accelerated organic carbon degradation parallel...

A dynamic chamber system consisting of a covering composting mixture (3 m in diameter, 2.2 height, 13 m3), ventilator for suction air into the chamber, and equipment to measure gas composition indicate temperature, was developed evaluation harmful emissions from such livestock waste composting. Fresh introduced through space between floor lower edge exhaust removed an outlet placed on top chamber. NH3, CH4 N2O concentrations were measured by Infrared Photoacoustic Detector (IPD, multi...

The relationship between the activity and community structure of microbes associated with oxidation ammonia in a full-scale rockwool biofilter was examined by kinetic, denaturing gradient gel electrophoresis (DGGE), sequence analyses. packing materials were sampled from two different depths at 3 sites. Estimated K(m) values similar among same sampling sites, while V(max) differed mid-point sample. lower depth this site had highest V(max). A correspondence analysis showed DGGE profile...

To obtain insight into the complex behaviour of denitrifying and total bacterial groups during nitrogen accumulation process in an ammonia-loaded biofiltration system.Denitrifying communities a laboratory-scale rockwool biofilter with intermittent water recirculation were analysed by using denaturing gradient gel electrophoresis targeting nosZ metabarcoding sequencing 16S rRNA gene. Gene abundance was evaluated quantitative PCR. The number increased from 6·59 × 106 to 3·33 108 copies per...

Anammox reaction requires nitrite and ammonium in the ratio of 1.1-1.3. However, controlling a partial nitrification process prior to anammox maintain this an influent reactor is not easy. In study, effect zeolite on was investigated determine method preservation case supply or ammonium. Up-flow column type reactors, filled with either non-woven fabric, were operated two-week intervals purified livestock wastewater containing nitrite. The showed significantly higher nitrogen removal rates...

Composting of livestock manure is a significant source greenhouse gases such as nitrous oxide (N2O), but the complexity N2O generation pathway hinders efforts to develop effective countermeasures. In this paper, we present one mitigation option for emissions from composting. Nitrite (NO2-) has an important role in generation. It been observed that composting are induced when NO2- accumulated during nitrification. Accordingly, our technique, nitrite-oxidizing bacteria (NOB), nitrifying...

ABSTRACT To remove the excess nitrogen and phosphorus in swine wastewater, an electrodialysis technique was applied to advanced treatment method. The laboratory‐scale wastewater system constructed for present study consisted of activated sludge process, as main unit, electrodialysis, unit. This operated 200 days processing performance evaluated. By approximately 99% NO 3 – PO 4 3– sludge‐treated water (AT solution) removed during operation. Furthermore, decreased color density AT solution at...

To investigate community shifts of amoA-encoding archaea (AEA) and ammonia-oxidizing bacteria (AOB) in biofilter under nitrogen accumulation process.A laboratory-scale rockwool with an irrigated water circulation system was operated for 436 days ammonia loading rates 49-63 NH(3) g m(-3) day(-1). The AEA AOB communities were investigated by denaturing gradient gel electrophoresis, sequencing real-time PCR analysis based on amoA genes. results indicated that changes abundance compositions...

Swine wastewater has a high potential for phosphorous recovery in Japan. The pH of swine increased up to approximately 8.5 with continuous aeration, and large part the soluble PO4-P, Mg Ca was crystallized. A reactor removing recovering from designed dual function i.e., crystallization through separation formed struvite by settling. However, dehydration, composting characterization process first needed before using sediment sludge, including struvite, on farmland, since it will settle along...