A5040702342- Composting and Vermicomposting Techniques
- Wastewater Treatment and Nitrogen Removal
- Odor and Emission Control Technologies
- Agriculture Sustainability and Environmental Impact
- Constructed Wetlands for Wastewater Treatment
- Microbial Community Ecology and Physiology
- Ruminant Nutrition and Digestive Physiology
- Extracellular vesicles in disease
- Ammonia Synthesis and Nitrogen Reduction
- RNA Interference and Gene Delivery
- Phosphorus and nutrient management
- Aluminum Alloys Composites Properties
- Advanced ceramic materials synthesis
- Gut microbiota and health
- Anaerobic Digestion and Biogas Production
- Genetic and phenotypic traits in livestock
- Calcium signaling and nucleotide metabolism
- SARS-CoV-2 and COVID-19 Research
- Indoor Air Quality and Microbial Exposure
- Agricultural Systems and Practices
- Soil and Water Nutrient Dynamics
- Immunotherapy and Immune Responses
- Mycorrhizal Fungi and Plant Interactions
- Polyamine Metabolism and Applications
- Wireless Communication Networks Research
Tokyo University of Science
2024
Japan International Research Center for Agricultural Sciences
2020-2023
Hirosaki University
2021-2023
Iwate University
2023
Hokkaido Agricultural Research Center
2009-2020
Tokyo Institute of Technology
1985-2016
Universidad Nacional Agraria La Molina
2008
Columbia University
2008
Tohoku University
2006
N2O is a powerful greenhouse gas contributing both to global warming and ozone depletion. While fungi have been identified as putative source of N2O, little known about their production this gas. Here we investigated the N2O-producing ability collection 207 fungal isolates. Seventy strains producing in pure culture were identified. They mostly species from order Hypocreales order-particularly Fusarium oxysporum Trichoderma spp.-and lesser extent orders Eurotiales, Sordariales,...
A molecular analysis of betaproteobacterial ammonia oxidizers and a N(2)O isotopomer were conducted to study the sources emissions during cow manure composting process. Much NO(2)(-)-N NO(3)(-)-N Nitrosomonas europaea-like amoA gene detected at surface, especially top pile, suggesting that these ammonia-oxidizing bacteria (AOB) significantly contribute nitrification which occurs surface layer compost piles. However, (15)N site preference within asymmetric molecule (SP = delta(15)N(alpha) -...
The relative contribution of fungi, bacteria, and nirS nirK denirifiers to nitrous oxide (N2O) emission with unknown isotopic signature from dairy manure compost was examined by selective inhibition techniques. Chloramphenicol (CHP), cycloheximide (CYH), diethyl dithiocarbamate (DDTC) were used suppress the activity nirK-possessing denitrifiers, respectively. Produced N2O surveyed isotopocule analysis, its 15N site preference (SP) δ18O values compared. Bacteria, nirS, gene abundances...
The greenhouse gas (GHG) [methane (CH4) and nitrous oxide (N2O)] mitigation effects of mixing dried grass into passively-aerated manure during the composting process (which accounts for 68.7% Japanese dairy management) were assessed. Gaseous emissions [CH4, N2O, carbon dioxide (CO2) ammonia (NH3)] from about 4 t fresh with or without 400 kg mixed in measured by dynamic chamber method. addition contributed to a decrease GHG 20.8 ± 1.3 g kg−1 volatile solids (VS) 5.4 1.4 VS (74.3% mitigation)...
Severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2), the virus responsible for coronavirus disease 2019 (COVID-19), has caused a global public health crisis. The E protein, structural protein found in this particle, is also known to be viroporin. As such, it forms oligomeric ion channels or pores host cell membrane. However, relationship between these two functions poorly understood. In study, we showed that roles of particle and viroporin formation are distinct. This study...
Livestock manure management systems can be significant sources of nitrous oxide (N2 O), methane (CH4 ), and ammonia (NH3 ) emissions. Many studies have been conducted to improve our understanding the emission processes identify influential variables in order develop mitigation techniques adapted each step (animal housing, outdoor storage, spreading land). The international project DATAMAN (http://www.dataman.co.nz) aims a global database on greenhouse gases O, CH4 NH3 emissions from chain...
To control ammonia (NH(3)) volatilization from the dairy cattle (Bos taurus) manure composting process, a compost pile was covered with mature and gas emissions evaluated using dynamic chamber system. The peak of NH(3) observed immediately after piling up reduced 196 to 62 mg/m(3) by covering compost. accumulation NH(4)-N also observed. Covering mixing had no effect on microbial community structure. However, over time structure changed because decrease in easily degradable organic compounds...
Summary The effects of cashew nut shell liquid (CNSL) feeding on the methane (CH 4 ) emission and ruminal microbiome Lai Sind beef cattle were investigated. Changes in production rumen by CNSL monitored a respiration chamber 16S rRNA gene amplicon sequencing respectively. results demonstrated that mitigated 20.2%–23.4% CH vivo without apparent adverse feed intake digestibility. fluid analysis revealed significant increase proportion propionate total short‐chain fatty acids. relative...
This study aimed to characterize microbial community dynamics in aerated cow manure slurry at different aeration intensities.Batch aerobic treatments were set up 5-l jar fermentor, each containing 3 l of slurry; the slurries subjected low, medium and high (50, 150 250 ml min(-1), respectively) for 9 days. Microbial composition was determined using terminal restriction fragment length polymorphism a clone library targeting 16S rRNA genes. High accelerated organic carbon degradation parallel...
This study investigated the effects of substituting rice straw with cassava in total mixed ration silage on feed intake, digestibility, rumen fermentation, ruminating activity, and energy balance. An balance experiment was conducted to determine nutrient utilization dairy bulls. Four Holstein Friesian (HF) crossbred young bulls (93.19% HF × 6.81% Native Thai; average age 12.08 ± 0.22 months body weight 266 9.80 kg) were used a 4 Latin square design four 28-d periods. The dietary treatments...
Abstract. Nitrogen isotope ratios (δ15N) of NH4+ in dairy manure compost piles with and without bulking agent (10 % w∕w) were compared to understand the effects use on nitrogen conversion during composting. The δ15N–NH4+ values each three pile zones (top, side core) also compared. At end process, showed significantly higher δ15N (17.7 ± 1.3 ‰) than (11.8 0.9 ‰), reflecting NH3 loss former. samples from top zone, especially agent, very high concentrations 15N (δ15N: 12.7–29.8 values,...
We measured the greenhouse gas (GHG) emissions following beef cattle feeding and evaluated manure management in northeast Thailand (Khon Kaen) to obtain country-specific emission factor (EF) replace Intergovernmental Panel on Climate Change (IPCC) default value. fed four Thai native their typical diet of region then used head-cage dynamic chamber methods measure enteric methane (CH 4 ) GHG during storage, respectively. The effect amending with rice straw manure’s was evaluated. microbiome...
Extracellular vesicles (EVs) are important mediators of intercellular communication. However, the methods available for distinguishing heterogeneity secreted EVs and isolating purifying them limited. This study introduced a HiBiT-tag to detect various EV markers, including CD63, CD9, Epidermal Growth Factor Receptor (EGFR), Flotilin1, Syndecan-1, investigated whether these marker-containing were capable binding differently charged column carriers. Four carriers, Diethylaminoethyl (DEAE),...