A5100395254- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Monoclonal and Polyclonal Antibodies Research
- Immune Response and Inflammation
- Glycosylation and Glycoproteins Research
- Immune cells in cancer
- Immunotherapy and Immune Responses
- Ion channel regulation and function
- Neuroscience and Neuropharmacology Research
- Signaling Pathways in Disease
- Memory and Neural Mechanisms
- Anesthesia and Neurotoxicity Research
- Cancer Mechanisms and Therapy
- Immunodeficiency and Autoimmune Disorders
- Chronic Lymphocytic Leukemia Research
- Pneumocystis jirovecii pneumonia detection and treatment
- Lymphoma Diagnosis and Treatment
- Epilepsy research and treatment
- Cell Adhesion Molecules Research
- melanin and skin pigmentation
- Complement system in diseases
- CAR-T cell therapy research
- Cancer Immunotherapy and Biomarkers
- Advanced biosensing and bioanalysis techniques
- Cancer Research and Treatments
Army Medical University
2024
Center for Cancer Research
2024
Sichuan University
2022
West China Hospital of Sichuan University
2022
Center for Drug Evaluation and Research
2013-2020
United States Food and Drug Administration
2016-2020
Kashi University
2020
Southern Medical University
2017-2018
Research & Diagnostic Antibodies (United States)
2013
Stanford University
2006-2007
Abstract Fc receptor–like (FCRL) 5 is a novel IgG binding protein expressed on B cells, with the capacity to regulate Ag receptor signaling. We assessed FCRL5 expression circulating cells from healthy donors and found that FCRL5+ are most enriched among atypical CD21−/lo/CD27− tissue-like memory (TLM) which abnormally expanded in several autoimmune infectious diseases. Using multicolor flow cytometry, TLM were express more CD11c inhibitory receptors than did FCRL5− subset. The homing...
Fc receptor-like (FCRL) 5 regulates B cell Ag receptor signaling and has been reported to bind aggregated IgG. Using surface plasmon resonance, we analyzed the interaction of native IgG samples with FCRL5, revealing a complex binding mechanism, where isotype is just one factor. FCRL5 bound IgG1 IgG4 ~1 μM KD, whereas IgG3 was magnitude weaker. However, IgG2 displayed wide range affinities, indicating that additional factors affect binding. We used panel 19 anti-FCRL5 mAbs defined reactivity...
Mannan binding lectin (MBL), initially known to activate the complement pathway and defend against infection, was recently shown be potentially involved in development of several types cancer; however, its exact role cancers, especially effect on tumor microenvironment remain largely unknown. Here, using a murine hepatocellular carcinoma (HCC) model, we showed that MBL component liver MBL-deficient (MBL–/–) mice exhibited an enhanced growth compared with wild-type (WT) mice. This phenomenon...
The B-cell response to antigen is critically regulated by co-receptors. CD21 (complement receptor 2) amplifies the linked its ligands, specific C3 fragments. In contrast, human Fc receptor-like 5 (FCRL5), a novel IgG receptor, was reported inhibit (BCR) signaling. Here, we show that and FCRL5 physically associate, suggesting immune complexes containing both fragment could simultaneously engage pre-assembled receptors. We found activating signaling molecules such as CD19, active PLCγ2 BTK...
Abstract Atypical memory B cells accumulate in chronic infections and autoimmune conditions, commonly express FCRL4 FCRL5, respective IgA IgG receptors. We characterized from tonsils on the basis of both FCRL5 expression, defining three subsets with distinct surface proteins gene expression. FCRL4+FCRL5+ had most discrete protein expression were enriched cell adhesion pathways, consistent functioning as tissue-resident cells. FCRL4−FCRL5+ expressed transcription factors immunoglobulin genes...
Purpose Propofol, an aesthetic agent in paediatric patients, results neurotoxicity the developing neurons. To reduce side effects of propofol, protective role miR-455-3p (microRNA-455-3p) rat brain was investigated.Materials and methods Primary hippocampal neurons were isolated from postnatal day 1 or 2 SD (Sprague-Dawley) rats. The exposed to various concentrations propofol (0, 10, 30, 50 μM) for 6 h. Propofol-induced cell viability assessed by MTT assay, expression levels EphA4...
Abstract Noninfectious liver injury, including the effects of drugs and diet, is a major cause diseases worldwide. The innate inflammatory response to hepatocyte death plays crucial role in outcome injury. Mannan-binding lectin (MBL) pattern recognition molecule immune system, which primarily produced by liver. MBL deficiency occurs with high frequency population reported associated predisposition infectious diseases. We here observed that genetic ablation strongly sensitizes mice sterile...
Abstract Background Rheumatoid arthritis (RA) is a complex autoimmune disease with multiple etiological factors, among which aberrant memory CD4 T cells activation plays key role in the initiation and perpetuation of disease. SIGIRR (single immunoglobulin IL-1R-related receptor), member IL-1 receptor (ILR) family, acts as negative regulator ILR Toll-like (TLR) downstream signaling pathways inflammation. The aim this study was to investigate potential roles on RA underlying cellular molecular...
Abstract FCRL4 and FCRL5 are two members of the human Fc receptor like family with capacity to regulate antigen signaling. was shown bind IgA, whereas binds IgG. Although expression on B cell subsets has each been individually evaluated, no study thus far addressed their potential co-expression. Here we report that three distinct subpopulations CD38lowIgD-memory cells exist in tonsils, one co-expresses FCRL5, another is single-positive, a third lacks both proteins. We found FCRL5+FCRL4+...
Cationic nanostructures have emerged as an adjuvant and antigen delivery system that enhances dendritic cell maturation, ROS generation, uptake then promotes antigen-specific immune responses. In recent years, retinoic acid (RA) has received increasing attention due to its effect in activating the mucosal response; however, order use RA a adjuvant, it is necessary solve problem of dissolution, loading, delivery. Here, we describe cationic nanoemulsion-encapsulated (CNE-RA) composed lipid...
Abstract Germinal centers (GCs) that form in mucosal sites are exposed to gut-derived factors have the potential influence homeostasis independent of antigen receptor-driven selective processes. The G-protein Gα13 confines B cells GC and limits development GC-derived lymphoma. We discovered Gα13-deficiency fuels reaction via increased mTORC1 signaling Myc protein expression specifically mesenteric lymph node (mLN). competitive advantage Gα13-deficient (GCBs) mLN was not dependent on T cell...
Objective: To study T cell immunology in patients with colorectal cancer, the molecular features of clonal expanded TIL and PBL were analyzed by assessment TCRβ gene reopertoire. Methods: The rearranged V-J genes chain amplified RT-PCR separated denaturation polyacrylamide sequencing gel eletrophoresis. PCR products sequenced directly to determine characteristics transcripts. Results: There oligoclonal transcripts V both from pre-operation patients, which all disappeared after operation....
Abstract B cell response is modulated by an array of co-receptors which transduce activating and/or inhibitory signals. The balance these stimuli will determine function and fate. Human Fc receptor-like 5 (FCRL5) a transmembrane protein containing immunoglobulin superfamily domains expressed distinct subsets cells. functional role FCRL5 poorly understood. However has been reported to be upregulated in several tumors, including hairy leukemia, mantle lymphoma multiple myeloma. cytoplasmic...
Abstract Rheumatoid arthritis (RA) is a complex autoimmune disease with multiple etiological factors, among which aberrant T-cell activation plays key role in the initiation and perpetuation of disease. The factors that trigger T cell abnormalities these inflammatory processes are incompletely understood. SIGIRR (single immunoglobulin IL-1R-related receptor) member IL-1 receptor (ILR) family distinct structural functional characteristics, acting as negative regulator ILR Toll-like (TLR)...