Lumpy skin disease (LSD) is a transboundary cattle caused by Capripoxvirus of the family Poxviridae. In Uganda, documented information on epidemiology rare and there no nationwide control plan, yet LSD endemic. This study set out to investigate seroprevalence lumpy determine risk factors for seropositivity, carrying cross-sectional in 21 districts Uganda.A total 2,263 sera samples were collected from 65 herds an indirect ELISA was used screen virus (LSDV) antibodies. We univariable...

Nagana (African Animal Trypanosomiasis-AAT) and tick-borne diseases (TBDs) constrain livestock production in most parts of sub-Saharan Africa. To this realisation, Uganda government set up an African trypanosomiasis (AT) control unit, which among other activities generates national tsetse priority maps using apparent density data. Such underestimate mechanically transmitted AAT thus ought to be refined actual AT prevalence We therefore out generate up-to-date cattle donkey data as well find...

Rhipicephalus microplus, an invasive tick species of Asian origin and the main vector Babesia species, is considered one most widespread ectoparasites livestock. The has spread from its native habitats on translocated livestock to large parts tropical world, where it replaced some local populations decoloratus ticks. Although was reported in Uganda 70 years ago, not been found any subsequent surveys. This study carried out update national distribution as a basis for tick-borne disease...

African swine fever (ASF) is a contagious viral disease, which can cause up to 100% mortality among domestic pigs. In Uganda there paucity of information on the epidemiology hence study was carried out elucidate patterns ASF outbreaks. Spatial and temporal analyses were performed with data collected monthly by district veterinary officers (DVOs) sent central administration at MAAIF from 2001 2012. Additionally, risk factors associated characteristics related disease assessed based...

African swine fever (ASF) is a contagious viral disease which can cause up to 100% mortality among domestic pigs leading serious socio-economic impact on people's livelihoods. ASF endemic in Uganda and there paucity of information the epidemiology disease. The major aim this study was determine seroprevalence prevalence virus (ASFV) apparently healthy slaughter at Wambizi slaughterhouse Kampala city, Uganda. We also estimated presence ASFV antibodies circulating antigens from selected...

Abstract Background Ticks and tick-borne diseases (TTBDs) are a significant threat to livestock production in sub-Saharan Africa. Transhumance pastoralism practiced Karamoja region other factors like cattle trade, communal grazing the presence of wildlife predispose TTBDs. Tick species abundance distribution data can be used as tool for early disease diagnosis inform tick control strategies. However, these north-eastern Uganda currently limited; previous surveys were relatively localized...

Antimicrobial resistance (AMR), a global threat driven by factors such as improper antimicrobial use in humans and animals, is projected to cause 10 million annual deaths 2050. For behavior change, public health messages must be tailored for diverse audiences. Generative AI may have the potential create culturally linguistically suited AMR awareness messages. This study assesses ChatGPT-4's capability crafting content. 4 veterinary professionals from linguistic cultural backgrounds...

African swine fever (ASF) is a highly lethal and economically significant disease of domestic pigs in Eastern Africa particularly Uganda where outbreaks regularly occur. Sequence analysis variable genome regions have been extensively used for molecular epidemiological studies virus (ASFV) isolates. By combining p72, P54 pB602L (CVR), high level resolution approach achieved viral discrimination. The major aim this study therefore, was to investigate the genetic relatedness ASF that occurred...

Lumpy skin disease (LSD) is a devastating transboundary viral of cattle which causes significant loss in production. Although this has been reported Uganda and throughout East Africa, there almost no information about its epidemiology, spatial or spatio-temporal distribution. We carried out retrospective study on the epidemiology LSD between years 2002 2016, using data outbreaks collected monthly by central government veterinary administration. Descriptive statistics were computed frequency...

Abstract Background Lumpy skin disease (LSD) is an infectious viral of cattle caused by a Capripoxvirus . LSD has substantial economic implications, with infection resulting in permanent damage to the affected animals which lowers their commercial value. In Uganda, endemic and cases are frequently reported government authorities. This study was undertaken molecularly characterize lumpy virus (LSDV) strains that have been circulating Uganda between 2017 2018. Secondly, aimed determine...

Pathogenic water dwelling protozoa such as Acanthamoeba spp., Hartmannella Naegleria Cryptosporidium spp. and Giardia are often responsible for devastating illnesses especially in children immunocompromised individuals, yet their presence prevalence certain environment sub-Saharan Africa is still unknown to most researchers, public health officials medical practitioners. The objective of this study was establish the pathogenic free-living amoeba (FLA), Queen Elizabeth Protected Area (QEPA)....

Effective management of foot and mouth disease (FMD) requires diagnostic tests to distinguish between infected vaccinated animals (DIVA). To address this need, several enzyme-linked immunosorbent assay (ELISA) platforms have been developed, however, these vary in their sensitivity specificity are very expensive for developing countries. Camelid-derived single-domain antibodies fragments so-called Nanobodies, demonstrated great efficacy the development serological diagnostics. This study...

African swine fever (ASF) is an infectious transboundary disease of domestic pigs and wild currently the most serious constraint to piggery in Uganda. The causative agent ASF a large double-stranded linear DNA virus with complex structure. There are twenty-four ASFV genotypes described date; however, Uganda, only IX X have been previously described. Inadequate outbreak investigation has contributed delayed establishment effective interventions aid control ASF. Continuous characterization...

Antimicrobial peptides (AMPs) constitute a broad range of bioactive compounds in diverse organisms, including fish. They are effector molecules for the innate immune response, against pathogens, tissue damage and infections. Still, AMPs from African Catfish, Clarias gariepinus , skin mucus largely unexplored despite their possible therapeutic role combating antimicrobial resistance. In this study, Catfish (ACAPs) were identified C. . Native extracted fish scrapings 10% acetic acid (v/v)...

Amidst rising cases of antimicrobial resistance, peptides (AMPs) are regarded as a promising alternative to traditional antibiotics. Even so, poor pharmacokinetic profiles certain AMPs impede their utility necessitating, careful assessment potential AMPs' absorption, distribution, metabolism, excretion, and toxicity (ADMET) properties during novel lead exploration. Accordingly, the present study utilized ADMET scores profile seven previously isolated African catfish (ACAPs). After profiling,...

Acanthamoeba is an emerging potentially pathogenic amoeba that has been receiving increasing attention worldwide as a reservoir and potential vector for the transmission of bacteria. It also associated with brain cell damage, keratitis skin irritation in humans. Its effects are more severe immunocompromised individuals. This study provides first time Uganda, information on prevalence genotypes environmental domestic (tap) water. A total 324 84 tap water samples were collected between...

This paper describes an assessment of the patterns peste des petits ruminants virus circulation in Karamoja subregion Uganda conducted to identify communities that maintain and inform development a targeted vaccination strategy. Participatory epidemiological methods were used develop operational hypothesis for PPR was subsequently validated through outbreak investigation genomics. The participatory included risk mapping with livestock owners, community animal health workers veterinarians...

Antimicrobial resistance remains a great threat to global health. In response the World Health Organizations’ call for action, nature has been explored novel and safe antimicrobial candidates. To date, fish have gained recognition as potential source of safe, broad spectrum effective therapeutics. The use computational methods design candidates industrial application however, lagging behind. fill gap contribute current fish-derived peptide repertoire, this study used Support Vector Machines...

Here, we report the results of a cross-sectional study designed to monitor circulation and genetic diversity foot mouth disease virus (FMDV) in Uganda between 2014 2017. In this study, 13,614 sera 2,068 oral-pharyngeal fluid samples were collected from cattle analysed determine FMDV seroprevalence, circulating serotypes their phylogenetic relationships. Circulation was evidenced by detection antibodies against non-structural proteins or viral isolations all districts sampled Uganda. Sequence...

Ticks are arthropod vectors of pathogens both Veterinary and Public health importance. Acaricide application which is currently the mainstay tick control hampered by high cost, need for regular selection multi-acaricide resistant populations. In light this, future approaches poised to rely on integration rational acaricide other methods such as vaccination. To contribute systematic research-guided efforts produce anti-tick vaccines, we carried out an in-silico analysis aquaporin-1 (AQP1)...

African swine fever (ASF) is a contagious viral disease, which can cause up to 100% mortality among domestic pigs. Pig production growing rapidly in Uganda East countries and not only source of food but also an important income for many people living the rural areas. Field diagnosis ASF depends on clinical signs has be confirmed laboratory since are pathognomonic. Diagnostic techniques focused serological tests detection antigen antibody, genomic DNA by polymerase chain reaction (PCR), virus...

African swine fever (ASF) is a highly lethal and economically significant disease of domestic pigs in Uganda where outbreaks regularly occur. There neither vaccine nor treatment available for ASF control. Twenty two virus (ASFV) genotypes (I - XXII) have been identified based on partial sequencing the C-terminus major capsid protein p72 encoded by B646L gene. The majority previously characterized Ugandan ASFV strains belong to genotype IX. aim current study was determine among asymptomatic...

The continued endemicity of foot and mouth disease virus (FMDV) in East Africa has significant implications for livestock production poverty reduction, yet its complex epidemiology endemic settings remains poorly understood. Identifying FMDV dispersal routes drivers transmission is key to improved control strategies. Environmental heterogeneity anthropogenic (e.g., demand animal products) can impact viral spread by influencing host movements. Here, we utilized serotype O VP1 genetic...