A5102802806- Macrophage Migration Inhibitory Factor
- Carbohydrate Chemistry and Synthesis
- Pneumocystis jirovecii pneumonia detection and treatment
- Chemical Reaction Mechanisms
- Insect Resistance and Genetics
- Plant Parasitism and Resistance
- Plant Molecular Biology Research
- Viral Infectious Diseases and Gene Expression in Insects
- Plant and animal studies
- Agricultural safety and regulations
- Antimicrobial Peptides and Activities
- Toxin Mechanisms and Immunotoxins
- Entomopathogenic Microorganisms in Pest Control
- Silk-based biomaterials and applications
- Biofuel production and bioconversion
- Periodontal Regeneration and Treatments
- Enzyme Catalysis and Immobilization
- Nanoparticle-Based Drug Delivery
- Plant nutrient uptake and metabolism
- Synthesis and Characterization of Heterocyclic Compounds
- Silicon Nanostructures and Photoluminescence
- Mycorrhizal Fungi and Plant Interactions
- Oral and Maxillofacial Pathology
- Allelopathy and phytotoxic interactions
- Analytical Chemistry and Sensors
University of Amsterdam
2020-2021
Norwegian Institute of Bioeconomy Research
2019
Stadtwerke Jülich (Germany)
2019
RWTH Aachen University
2016-2019
University of Groningen
2015-2017
Pharmaceutical Biotechnology (Czechia)
2016
Isfahan University of Medical Sciences
2011
Using a family of cationic gold nanoparticles (NPs) with similar size and charge, we demonstrate that proper surface engineering can control the nature identity protein corona in physiological serum conditions. The coronas were highly dependent on hydrophobicity arrangement chemical motifs NP surface. NPs uptaken macrophages corona-dependent manner, predominantly through recognition specific complement proteins corona. Taken together, this study shows functionality be used to tune formed...
Abstract Most plants associate with beneficial arbuscular mycorrhizal (AM) fungi that facilitate soil nutrient acquisition. Prior to contact, partner recognition triggers reciprocal genetic remodelling enable colonisation. The plant Dwarf14-Like (D14L) receptor conditions pre-symbiotic perception of AM fungi, and also detects the smoke constituent karrikin. D14L-dependent signalling mechanisms, underpinning symbiosis are unknown. Here, we present identification a negative regulator from...
Abstract The Michael-type addition reaction is widely used in organic synthesis for carbon–carbon bond formation. However, biocatalytic methodologies this type of are scarce, which related to the fact that enzymes naturally catalysing bond-forming additions rare. A promising template develop new biocatalysts formation enzyme 4-oxalocrotonate tautomerase, exhibits promiscuous activity. Here we present mutability landscapes expression, tautomerase and activities, enantioselectivity...
Parasitic plants are that connect with a haustorium to the vasculature of another, host, plant from which they absorb water, assimilates, and nutrients. Because this parasitic lifestyle, need coordinate their lifecycle host. have evolved number host detection/host response mechanisms germination in chemical signals one major families plants, Orobanchaceae, is striking example. In update review, we discuss these stimulants. We review different compound classes function as stimulants, how...
A bifunctional dipeptide can help to reduce pesticide use while preserving crop productivity.
Abstract The enzyme 4‐oxalocrotonate tautomerase (4‐OT), which has a catalytic N‐terminal proline residue (Pro1), can promiscuously catalyze various carbon–carbon bond‐forming reactions, including aldol condensation of acetaldehyde with benzaldehyde to yield cinnamaldehyde, and Michael‐type addition wide variety nitroalkenes valuable γ‐nitroaldehydes. To gain insight into how 4‐OT catalyzes these unnatural we carried out exchange studies in D 2 O, X‐ray crystallography studies. former...
Abstract The enzyme 4‐oxalocrotonate tautomerase (4‐OT), which catalyzes enol–keto tautomerization as part of a degradative pathway for aromatic hydrocarbons, promiscuously various carbon–carbon bond‐forming reactions. These include the aldol condensation acetaldehyde with benzaldehyde to yield cinnamaldehyde. Here, we demonstrate that 4‐OT can be engineered into more efficient aldolase this reaction, >5000‐fold improvement in catalytic efficiency ( k cat / K m ) and >10 7 ‐fold change...
The enzyme 4-oxalocrotonate tautomerase (4-OT), which in nature catalyzes a tautomerization step as part of catabolic pathway for aromatic hydrocarbons, was found to promiscuously catalyze different types aldol reactions. These include the self-condensation propanal, cross-coupling propanal and benzaldehyde, pyruvate, intramolecular cyclizations hexanedial heptanedial. Mutation catalytic amino-terminal proline (P1A) greatly reduces 4-OT's aldolase activities, whereas mutation another active...
Abstract Main conclusion The sunflower sesquiterpene lactones 8-epixanthatin and tomentosin can bind to the hydrophobic pocket of KAI2 with an affinity much higher than for exogenous ligand KAR. Sesquiterpene (STLs) are secondary plant metabolites a wide range biological, such as anti-microbial, activities. Intriguingly, STLs have also been implicated in development: several Asteraceae, STL levels correlate photo-inhibition hypocotyl elongation. Although this effect was suggested be due...
The enzyme 4-oxalocrotonate tautomerase (4-OT) from Pseudomonas putida mt-2 takes part in a catabolic pathway for aromatic hydrocarbons, where it catalyzes the conversion of 2hydroxyhexa-2,4-dienedioate into 2-oxohexa-3-enedioate. This can also promiscuously catalyze carbon–carbon bond-forming reactions, including various types aldol by using its amino-terminal proline as key catalytic residue. Here, we used systematic mutagenesis to identify two hotspots 4-OT (Met45 and Phe50) at which...
The aim of this study was to isolate and express the randomly mutated α- amylase gene from B. subtilis strain 168. BS168F: 5 ′ -gtgtcaagaatgtttgc-3 BS168R: 3 -gttttgttaaaagatga-5 primers were used amplify using following cycle in error-prone PCR method: 94 ° C for 30 s, 40 2 min, 72 min cycles that followed with as a post cycle. E. coli XL1 blue host plasmid construction. Amylase enzyme activity assay performed continuous spectrophotometric procedures. Results sequencing showed sequence...
<ns4:p><ns4:bold>Background: </ns4:bold>The eclepins are a group of root-exuded compounds that induce the hatching cyst nematodes, economically relevant pests for crops such as soybean and potato. From an evolutionary standpoint beneficial effect would be expected plant.</ns4:p><ns4:p> <ns4:bold>Methods: </ns4:bold>To identify this effect, we analysed transcriptome two plant species in response to treatment with eclepin. One eclepins, solanoeclepin A (solA), produced by members...