A5067466882- Melanoma and MAPK Pathways
- Ocular Oncology and Treatments
- Microtubule and mitosis dynamics
- Hippo pathway signaling and YAP/TAZ
- melanin and skin pigmentation
- Retinal Development and Disorders
- bioluminescence and chemiluminescence research
- Advanced Electron Microscopy Techniques and Applications
- Advanced Fluorescence Microscopy Techniques
- Multiple Myeloma Research and Treatments
- Molecular Biology Techniques and Applications
- RNA regulation and disease
- Ubiquitin and proteasome pathways
- Stroke Rehabilitation and Recovery
- Child and Adolescent Psychosocial and Emotional Development
- Cellular transport and secretion
- Quinazolinone synthesis and applications
- Suicide and Self-Harm Studies
- Electron and X-Ray Spectroscopy Techniques
- Transcranial Magnetic Stimulation Studies
- Endometrial and Cervical Cancer Treatments
- Cervical Cancer and HPV Research
- Electroconvulsive Therapy Studies
- Peptidase Inhibition and Analysis
- interferon and immune responses
University of Iowa
2024
University of Utah
2012-2023
University of Nebraska Medical Center
2022
Institute of Molecular Medicine
2017
Intermountain Healthcare
2015
Howard Hughes Medical Institute
2012-2013
Abstract Purpose: Mutational activation of GNAQ or GNA11 (GNAQ/11), detected in >90% uveal melanomas, leads to constitutive oncogenic pathways, including MAPK and YAP. To date, chemo- pathway-targeted therapies, either alone combination, have proven ineffective the treatment patients with metastatic melanoma. Experimental Design: We tested efficacy chloroquine hydroxychloroquine, combination pathway inhibition GNAQ/11-mutated cells vitro vivo identified mechanisms MEK1/2 inhibitor...
The devastating sequelae of diabetes mellitus include microvascular permeability, which results in retinopathy. Despite clinical and scientific advances, there remains a need for new approaches to treat Here, we have presented possible treatment strategy, whereby targeting the small GTPase ARF6 alters VEGFR2 trafficking reverses signs pathology 4 animal models that represent features diabetic retinopathy fifth model ocular pathological angiogenesis. Specifically, determined same signaling...
The activation of the small GTPase ARF6 has been implicated in promoting several pathological processes related to vascular instability and tumor formation, growth, metastasis. also plays a vital role during embryonic development. Recent studies have suggested that carries out these disparate functions primarily by controlling protein trafficking within cell. helps direct proteins intracellular or extracellular locations where they function normal cellular responses development later life....
Our objective was to determine the extent of testing and costs solely related diagnosis (the diagnostic odyssey) in a cohort children with inherited leukodystrophies.We determined all inpatient outpatient laboratory testing, including brain MRIs obtained for purpose diagnosis, over an 8-year time period retrospective population leukodystrophies. Costs were from activity-based cost accounting system standardized 2013 constant US dollars.Each patient had on average 20 tests (range 2-42 tests),...
Inherited leukodystrophies are a group of neurological disorders with significant morbidity and mortality. Children their families can experience lengthy diagnostic odysseys; however, there is no data on the charges related to testing for diagnosis in leukodystrophy patients, compared approaches using next‐generation sequencing (NGS). Our objective was determine determination diagnosis, overall yield testing, patients. We determined quantified all inpatient outpatient lab including brain...
Mapping the distribution of proteins is essential for understanding function in a cell. Fluorescence microscopy extensively used protein localization, but subcellular context often absent fluorescence images. Immuno-electron microscopy, on other hand, can localize proteins, technique limited by lack compatible antibodies, poor preservation morphology and because most antigens are not exposed to specimen surface. Correlative approaches acquire image from whole cell first, either...
Mapping the distribution of proteins is essential for understanding function in a cell. Fluorescence microscopy extensively used protein localization, but subcellular context often absent fluorescence images. Immuno-electron microscopy, on other hand, can localize proteins, technique limited by lack compatible antibodies, poor preservation morphology and because most antigens are not exposed to specimen surface. Correlative approaches acquire image from whole cell first, either...
Abstract Background As adolescent suicide rates have increased, there has been a nationwide increase in legislation requiring schools to create prevention policies. In Utah, school districts must implement youth program for grades 7–12. Currently, no systematic analyses of Utah district Thus, we developed new evaluation instrument, the School Suicide Policy Evaluation Tool (SSPET). Methods The SSPET was derived from “Model District Policy” and evaluates criteria on prevention, intervention,...
Background: As adolescent suicide rates have increased, there has been a nationwide focus on legislation requiring school districts to create prevention policies. There is lack of tools make quantitative assessment the quality and breadth school-district Thus, we developed proof-of-concept instrument, School District Suicide Policy Evaluation Tool (SDSPET). Methods: The SDSPET derived from “Model Prevention,” document compiled by four nationally recognized organizations. preliminary...
<p>Supplementary Materials and Methods</p>
<p>Response of uveal melanoma cell lines to MEK1/2 inhibition</p>
<p>Galpha and MEK1/2 inhibition in combination with Baf A1 CQ MP41 cells</p>
<p>YAP activation in OMM1 cells</p>
<p>Trametinib in combination with macropinocytosis or mitophagy inhibition</p>
<p>YAP requirement in MP41 cells</p>
<p>Validation of hepatic tumor model</p>
<p>Immunofluorescence analysis of autophagy in OMM2.5 cells with or without trametinib</p>
<p>Bioluminescent images of hepatic tumors</p>