galactosamine-protein acetylgalactosaminyltransferase (EC 2.4.1.41).Partial purification from salivary gland extracts and acceptor specificity of this purified transferase have been reported (9).Although the preparation was far homogeneous, it found that enzyme acted preferentially on high molecular weight acceptors such as apomucins (deglycosylated submaxillary mucins) or a protein bovine myelin (A1 protein).We undertook to purify characterize an ascites hepatoma, AH 66, in order obtain...

Development of new therapeutic strategies for breast cancer is urgently needed due to the sustained emergence drug resistance, tumor recurrence and metastasis. To gain a novel insight into approaches fight against cancer, cytocidal effects hellebrigenin (Helle) arenobufagin (Areno) were investigated in human estrogen receptor (ER)-positive cell line MCF-7 triple-negative MDA-MB-231. Helle exhibited more potent cytotoxicity than Areno both cells, cells susceptible drugs comparison with...

Chemo-sensitivity of two-dimensional (2D) monolayers and three-dimensional (3D) spheroids human breast cancer MCF-7 cells were investigated.MCF-7 cultured in or established using a thermo-reversible gelatin polymer, the presence daunorubicin, docetaxel, As2S2 Cell proliferation was examined by Counting Kit-8 assay.Daunorubicin, dose-dependently decreased cell both 2D- 3D-culture systems. The 3D less sensitive to these agents than 2D cells. Verapamil, an inhibitor P-glycoprotein, partially...

Proline-producing strains of Serratia marcescens were more osmotolerant than wild-type strains. Growth inhibition by proline analogs was significantly enhanced increasing the osmotic stress medium. Mutants resistant to azetidine-2-carboxylate derived from a proline-producing strain, SP126, under high condition. One mutants, strain SP187, produced 56 mg L-proline per ml medium containing sucrose and urea. This amount ca. 3 times larger that SP126. The intracellular glutamate content which...

The need for novel therapeutic strategies breast cancer remains paramount given the sustained development of drug resistance, tumor recurrence and metastasis. Trivalent arsenic derivatives (arsenite, AsIII) with remarkable clinical efficacy in acute promyelocytic leukemia has been demonstrated to exhibit inhibitory effect against cells. We recently that AsIII plus tetrandrine (Tetra), a Chinese plant-derived alkaloid, exerted potent antitumor activity human cells, however, underlying...

Oral mucositis (OM) is a common side effect in patients with cancer receiving chemotherapy and radiotherapy; however, no salivary mediator known to be associated OM. We aimed determine candidate inflammatory mediators potentially OM cancer. To this end, we compared the relationships between grade, oral mucosal dryness, (Interleukin (IL)-1β, IL-6, IL-10, IL-12p70, tumor necrosis factor (TNF), prostaglandin E2, vascular endothelial growth factor) healthy volunteers (HV). collected saliva...

Prostaglandin F2α (PGF2α) analog formulations are the most commonly used drugs for glaucoma treatment. They known to be superior β-blockers reducing intraocular pressure and can effective all through day. Because of action, topical contraindicated patients with bronchial asthma. PGF2α is also act as a constrictor respiratory tract. The present study aims analyze relationship between analogs In addition, we utilized combined both contents evaluate comparison analogs. Data from Japanese...

Some aspects regarding the use of antiplatelet agents after coil embolization for subarachnoid hemorrhage during admission remain unclear. This study used diagnostic procedure combination (DPC) data to investigate safety and prognostic effects aspirin monotherapy P2Y12 inhibitor therapy on bleeding events. cross-sectional Japanese DPC assess patients who were hospitalized with received between April 2016 March 2020 (n = 4421). The (A group, n 2848) (AP 1573) groups compared. primary...

The authors have previously reported that homologous immunoglobulin (Ig)G reduces the occurrence of dextran sulfate sodium (DSS)-induced colitis, mainly by suppressing recruitment immunocompetent cells into colitis lesions. However, mechanisms cell and its suppression IgG remain unclear. In addressing these questions, this study focused on activation T in presence macrophages. found [3H]-thymidine uptake from DSS-induced mice, but not normal was significantly enhanced when cultured with...

Proteinuria is a major side-effect of the anti-tumor drug bevacizumab, although its incidence and risk factors in real world are still unclear. Although renin-angiotensin-aldosterone system inhibitors used clinically to prevent proteinuria, their efficacy remains The aim present study was reveal bevacizumab-induced proteinuria examine effectiveness antihypertensive drugs preventing proteinuria. We conducted retrospective cohort using National Hospital Organization Clinical Data Archives...

In China, arsenic disulfide (As2S2) has been used for the treatment of hematological malignancies. The present study aimed to evaluate effects As2S2 on human breast cancer MCF‑7 cell line cultured in both two‑dimensional (2D) monolayers and three‑dimensional (3D) spheroids explore its therapeutic potential treatment. Cellular viability induction apoptosis were examined with a counting kit‑8 (CCK‑8) assay flow cytometric analysis, respectively. Alterations expression levels...

A norleucine-resistant mutant was derived from an isoleucine-valine auxotroph of a leucine accumulator Serratia marcescens. The could accumulate norleucine norvaline in the medium without addition methionine, which antagonized norleucine. This constitutively formed homoserine-O-transsuccinylase.

Three-dimensionally (3D) cultured tumor cells (spheroids) exhibit more resistance to therapeutic agents than the in traditional two-dimensional (2D) system (monolayers). We previously demonstrated that arsenic disulfide (As 2 [Formula: see text] exerted significant anticancer efficacies both 2D- and 3D-cultured MCF-7 cells, whereas 3D spheroids were shown be resistant As S treatment. L-buthionine-(S, R)-sulfoximine (BSO), an inhibitor of glutathione (GSH) synthesis, has been regarded a...

Hochuekkito (HET), Juzentaihoto (JTT), and Ninjin’yoeito (NYT) have been used as Hozai, a group of traditional Japanese herbal medicines, to treat physically mentally weak cancer patients. Their compositions are quite different, pharmaceutical companies using different types or quantities herbs for formulations with the same name. Here, we compared immunological differences between HET, JTT, NYT respect induced T cell subsets cytokines. Peripheral blood mononuclear cells (PBMCs) were...

Severe nausea and vomiting induced by antineoplastics diminish the patient’s quality of life ability to tolerate further chemotherapy. Ramosetron hydrochloride is a 5-HT3 receptor antagonist, which has an active metabolite (M-1), expected be useful in inhibition chemotherapy-induced vomiting. In present study, order analyze pharmacological effect ramosetron comprehensive manner, we estimated occupancy after intravenous administration using pharmacokinetic parameters dissociation constants...

The drug-binding properties of human alpha-foetoprotein (alpha FP) were investigated by a fluorescence-spectral method. Human alpha FP was shown to bind albumin's site I marker (warfarin, phenylbutazone), II (L-tryptophan), but not III (cholic acid, digoxin). binding towards lower alcohols examined, and this seems depend partly on the hydrophobicity ligands. is discussed in comparison with serum albumin or rat FP.

Cepharanthine (CEP) is a bis-bynzelisoquinoline alkaloid from the same class as anticancer agent tetrandrine (TET). However, effects of CEP against breast cancer have not been extensively studied, despite its long therapeutic history with low toxicity other types cancer. 3D culture systems more accurately mimic human body and address limitations determining drug effectiveness compared 2D systems. In present study, antitumor activities TET were in triple-negative (TNBC) MDA-MB-231 estrogen...

Approximately a decade has passed since the addition of venous thromboembolism to list significant adverse reactions antipsychotic drugs. However, only few studies have investigated relationship between use and in Japanese population. We aimed evaluate risk recurrent users drugs update evidence on A cross-sectional retrospective analysis data from large claims database, managed by Medical Data Vision Co. Ltd., was conducted. Adult patients who experienced October 2014 September 2018 acute...

The effects of hypnotics are one the many causes falls.However few studies have been done on a relationship between falls and use in Japan.We studied 109 patients who had experienced hospital.The male to female ratio was 6:4,mean age 67 years,22% them hepatic dysfunction,and 12% renal dysfunction.Among them,38.5% were hypnotics,and other frequently prescribed drugs diuretics,analgesic antipyretics laxatives.The incidence for higher subjects ultra-short-acting type (1.6 6.2%) than those...

Pharmacological effect after intravenous (i.v.) or subcutaneous (s.c.) administration of human recombinant granulocyte colony stimulating factor (rhG-CSF) was evaluated by using a physiologically based pharmacokinetic/pharmacodynamic model. The increase neutrophil counts in blood s.c. rhG-CSF (0.5-1.0 μg/kg) larger than that i.v. the same dose, while area under curves plasma concentration smaller (Azuma et al., J. Clin. Therap. Med., 5, 1579-1603, 1989). Based on model considering metabolic...

Clinical evidence of thiamine-related neuropsychiatric symptoms, including the initial stage, is limited because serum thiamine levels tend to be evaluated only for patients who develop severe symptoms suspected related deficiency. This study aimed evaluate relationship between decline and effect chemotherapy on in gastrointestinal hematological cancer receiving chemotherapy.We retrospectively identified 87 were diagnosed with cancers at our hospital. We risk factors associated...

Abstract Objectives Patients with hematological cancer receiving chemotherapy have a high risk of thiamine deficiency due to accelerated usage by tumor cells. Mild or severe can lead varying degrees neurological symptoms. We evaluated the relationship between and symptoms, including mild nonspecific influence on serum levels in patients chemotherapy. Materials Methods retrospectively identified 42 diagnosed at our hospital, using electronic medical records collected from March 2019 2020....

The measurement of the uniformity preparations is an important component quality assessment. This study was conducted to evaluate appropriate mixing time for external using a planetary centrifugal mixer. Steroid ointment and white petrolatum (5 g each) were added 13-mL container mixed mixer (NRE-120®, THINKY) at rotation speed 800 rpm revolution 2000 rpm. We sampled 0.1 mixture from three spots (the top, middle, bottom portions) container, measured steroid content, evaluated product mixtures...