A5086176277- Chemokine receptors and signaling
- Adipokines, Inflammation, and Metabolic Diseases
- Atherosclerosis and Cardiovascular Diseases
- Cardiac Valve Diseases and Treatments
- Immune Response and Inflammation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Aortic Disease and Treatment Approaches
- Inflammation biomarkers and pathways
- Cell Adhesion Molecules Research
- Nitric Oxide and Endothelin Effects
- S100 Proteins and Annexins
- Immune cells in cancer
- RNA Interference and Gene Delivery
- Protease and Inhibitor Mechanisms
- Cardiovascular Disease and Adiposity
- Connective tissue disorders research
- Cancer-related molecular mechanisms research
- Coronary Interventions and Diagnostics
- Cytokine Signaling Pathways and Interactions
- Nanoparticle-Based Drug Delivery
- Curcumin's Biomedical Applications
- RNA Research and Splicing
- Adipose Tissue and Metabolism
- Blood Coagulation and Thrombosis Mechanisms
- Lipid Membrane Structure and Behavior
Institute of Parasitology
2002-2024
Emergency University
2024
Carol Davila University of Medicine and Pharmacy
2024
Romanian Academy
2003-2016
Ludwig-Maximilians-Universität München
2012
Vlaams Instituut voor Biotechnologie
2012
RWTH Aachen University
2012
Background: Lipopolysaccharide (LPS) is widely recognized as a potent activator of monocytes/macrophages, and its effects include an altered production key mediators, such inflammatory cytokines chemokines. The involvement G i protein in mediating LPS has been demonstrated murine macrophages various cell types human origin. Purpose: aim the present work was to evaluate potential -protein inhibitor encapsulated liposomes reducing induced by monocytes/macrophages. Materials methods: Guanosine...
Neutrophils have been classically viewed as a homogenous population. Recently, neutrophils were phenotypically classified into pro-inflammatory N1 and anti-inflammatory N2 sub-populations, but the functional differences between two subtypes are not completely understood. We aimed to investigate phenotypic neutrophils, identify potential contribution of S100A9 alarmin in neutrophil polarization. describe distinct transcriptomic profiles neutrophils. Compared N2, exhibited: i) higher levels...
Following myocardial infarction (MI), blood neutrophils quickly and extensively infiltrate the heart, where they are temporally polarized into pro-inflammatory (N1) anti-inflammatory (N2) subpopulations. Neutrophil transmigration is rapidly followed by accrual of macrophages (MACs), which believed to undergo local phenotypic transformations from pro-healing MACs that mediate inflammation resolution. We hypothesized N2 can reprogram toward a healing phenotype with increased efferocytosis...
The chemokine receptor CX(3)CR1 is an inflammatory mediator in vascular diseases. On platelets, its ligation with fractalkine (CX(3)CL1) induces platelet activation followed by leukocyte recruitment to activated endothelium. Here, we evaluated the expression and role of platelet-CX(3)CR1 during hyperlipidemia injury.The existence on platelets at mRNA protein level was analyzed RT-PCR, quantitative (q)PCR, FACS analysis, Western blot. Elevated detected human after and, along increased binding...
Abstract Calcific aortic valve disease (CAVD)—the most common valvular heart disease—is accelerated in diabetes and has no pharmacotherapy. Although it is known that early CAVD associated with inflammation osteogenesis, the molecular mechanisms involved diabetes‐associated still need to be uncovered. In this context, we have developed a 3D construct based on gelatin populated human endothelial cells (VEC) interstitial (VIC) evaluated effect of high glucose (HG) concentration osteogenic...
The excessive and uncontrolled release of neutrophil extracellular traps (NETs) is increasingly linked to the pathogenesis various inflammatory diseases, cardiovascular disorders, cancers. Real-time, non-invasive detection NETs crucial for understanding their role in disease progression developing targeted therapies. Current methods often lack necessary specificity resolution, particularly vivo ex settings. To address this, we have developed novel near-infrared squaraine-peptide conjugates...
The immune-vascular crosstalk is a critical factor in cancer and cardiovascular diseases, driving disease progression through inflammatory, remodeling, angiogenic pathways. Despite recent progress, the underlying molecular mechanisms that govern interplay between vascular immune cells remain elusive. Understanding these interactions provides opportunities for developing novel therapeutic strategies targeting components to improve treatment efficacy patient outcomes. In this context, our work...
Abstract Objectives To prepare and characterize in vitro vivo lipid nanoemulsions (LN) loaded with curcumin (Cm) functionalized a cell-penetrating peptide. Methods Curcumin-loaded (CmLN) nona-arginine peptide (R9-CmLN) have been obtained, characterized optimized for size, entrapment efficiency Cm release. The interaction of R9-CmLN human endothelial cells (HEC) was investigated using cultured EA.hy926 cells, biodistribution studies were performed C57BL6 mice. Key findings When used...
Summary The major complication of diabetes mellitus is accelerated atherosclerosis that entails an inflammatory process, in which fractalkine and monocyte chemotactic protein-1 (MCP-1) play a key role.We investigated the effect diabetes-associated high glucose (HG) on these chemokines signalling mechanisms involved human aortic smooth muscle cells (SMC). Exposure SMC to HG resulted increase MCP-1 expression activated mitogen-activated protein kinase (MAPK) pathway, process associated with...
Calcific aortic valve disease (CAVD), a degenerative characterized by inflammation, fibrosis and calcification, is accelerated in diabetes. Hyperglycemia contributes to this process mechanisms that still need be uncovered. We have recently developed 3D model of the human based on gelatin methacrylate revealed high glucose (HG) induced osteogenic molecules increased calcium deposits pro-osteogenic environment. To further understand events leading calcification diabetic conditions CAVD, we...
Despite neutrophil involvement in inflammation and tissue repair, little is understood about their inflammatory status acute coronary syndrome (ACS) patients with poor outcomes. Hence, we investigated the potential correlation between markers prognosis of ACS with/without diabetes explored whether neutrophils demonstrate a unique phenotype experiencing an adverse in-hospital outcome. The study enrolled 229 or without diabetes. Poor evolution was defined as either death, left ventricular...
Enoxaparin is a low molecular weight heparin, widely accepted as anticoagulant or antithrombotic drug, and likely to have role in acute inflammation. To evaluate the anti-inflammatory potential of enoxaparin, we investigated direct effect drug on activation endothelial cells. For this purpose set up an vitro system which cultured valvular cells (VEC) activated by tumor necrosis factor alpha lipopolysaccharide were exposed monocytic cell line; these conditions induced significant adhesion...
Resistin is a cytokine which plays an important role in cardiovascular disease by influencing systemic inflammation and endothelial activation. In human cells (HEC) it increases the expression of P‐selectin fractalkine, enhances monocyte adhesion antioxidant mechanisms. This study investigated whether natural antioxidants curcumin (CC) extract Morus alba leaves (MA) have protective effects resistin‐activated HEC. HEC were exposed to 100 ng/mL resistin for 6 18 h absence or presence MA CC...
Inflammation is a common process associated with numerous vascular pathologies. We hypothesized that targeting the inflamed endothelium by coupling peptide high affinity for P-selectin to surface of dexamethasone-loaded lipid nanoemulsions will highly increase their specific binding activated endothelial cells (EC) and reduce cell activation. developed characterized directed towards (PLN-Dex) monitored anti-inflammatory effects in vitro using cultured EC (EA.hy926 cells) vivo mouse model...