A5060028630- Cardiac Valve Diseases and Treatments
- Aortic Disease and Treatment Approaches
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- S100 Proteins and Annexins
- Immune Response and Inflammation
- Sepsis Diagnosis and Treatment
- Diabetic Foot Ulcer Assessment and Management
- Connective tissue disorders research
- Cancer and Skin Lesions
- Conducting polymers and applications
- Fuel Cells and Related Materials
- Parathyroid Disorders and Treatments
- Cardiovascular Function and Risk Factors
- Phagocytosis and Immune Regulation
- Pressure Ulcer Prevention and Management
- Immune cells in cancer
- Ocular Oncology and Treatments
- Aortic Thrombus and Embolism
- Advanced Glycation End Products research
- Coronary Interventions and Diagnostics
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Electrospun Nanofibers in Biomedical Applications
- Cancer Diagnosis and Treatment
- Wound Healing and Treatments
Emergency University
2024
Carol Davila University of Medicine and Pharmacy
2024
Institute of Parasitology
2024
Neutrophils have been classically viewed as a homogenous population. Recently, neutrophils were phenotypically classified into pro-inflammatory N1 and anti-inflammatory N2 sub-populations, but the functional differences between two subtypes are not completely understood. We aimed to investigate phenotypic neutrophils, identify potential contribution of S100A9 alarmin in neutrophil polarization. describe distinct transcriptomic profiles neutrophils. Compared N2, exhibited: i) higher levels...
Following myocardial infarction (MI), blood neutrophils quickly and extensively infiltrate the heart, where they are temporally polarized into pro-inflammatory (N1) anti-inflammatory (N2) subpopulations. Neutrophil transmigration is rapidly followed by accrual of macrophages (MACs), which believed to undergo local phenotypic transformations from pro-healing MACs that mediate inflammation resolution. We hypothesized N2 can reprogram toward a healing phenotype with increased efferocytosis...
Abstract Calcific aortic valve disease (CAVD)—the most common valvular heart disease—is accelerated in diabetes and has no pharmacotherapy. Although it is known that early CAVD associated with inflammation osteogenesis, the molecular mechanisms involved diabetes‐associated still need to be uncovered. In this context, we have developed a 3D construct based on gelatin populated human endothelial cells (VEC) interstitial (VIC) evaluated effect of high glucose (HG) concentration osteogenic...
The immune-vascular crosstalk is a critical factor in cancer and cardiovascular diseases, driving disease progression through inflammatory, remodeling, angiogenic pathways. Despite recent progress, the underlying molecular mechanisms that govern interplay between vascular immune cells remain elusive. Understanding these interactions provides opportunities for developing novel therapeutic strategies targeting components to improve treatment efficacy patient outcomes. In this context, our work...
Calcific aortic valve disease (CAVD), a degenerative characterized by inflammation, fibrosis and calcification, is accelerated in diabetes. Hyperglycemia contributes to this process mechanisms that still need be uncovered. We have recently developed 3D model of the human based on gelatin methacrylate revealed high glucose (HG) induced osteogenic molecules increased calcium deposits pro-osteogenic environment. To further understand events leading calcification diabetic conditions CAVD, we...
Despite neutrophil involvement in inflammation and tissue repair, little is understood about their inflammatory status acute coronary syndrome (ACS) patients with poor outcomes. Hence, we investigated the potential correlation between markers prognosis of ACS with/without diabetes explored whether neutrophils demonstrate a unique phenotype experiencing an adverse in-hospital outcome. The study enrolled 229 or without diabetes. Poor evolution was defined as either death, left ventricular...
Introduction Acute insulin resistance (IR) and hyperglycemia are frequently observed during acute myocardial infarction (AMI), significantly influencing both immediate long-term patient outcomes, irrespective of diabetic status. Neutrophilia increased neutrophil activity, which common in these scenarios, have been associated with poorer prognoses, as demonstrated our recent findings. While it is well established that neutrophils stress-induced exacerbate inflammation hinder recovery, the...
Parathyroid hormone (PTH) is a key regulator of calcium, phosphate and vitamin D metabolism. Although it has been reported that aortic valve calcification was positively associated with PTH, the pathophysiological mechanisms direct effects PTH on human valvular cells remain unclear. Here we investigated if induces endothelial (VEC) dysfunction in turn could impact switch interstitial (VIC) to an osteoblastic phenotype. Human VEC exposed were analyzed by qPCR, western blot, Seahorse, ELISA...
Current trends in the development of wound dressings are oriented towards use biopolymer-based materials, due to their unique properties such as non-toxicity, hydrophilicity, biocompatibility and biodegradability, that have advantageous therapeutic characteristics. In this regard, present study aims develop hydrogels based on cellulose dextran (CD) reveal anti-inflammatory performance. This purpose is achieved by incorporating plant bioactive polyphenols (PFs) CD hydrogels. The assessments...
Background: Valvular endothelial cells (VEC) have key roles in maintaining valvular integrity and homeostasis, dysfunctional VEC are the initiators major contributors to aortic valve disease diabetes. Previous studies shown that HG stimulated an inflammatory phenotype VEC. Inflammation was induce mesenchymal transition (EndMT), a process extensively involved many pathologies, including calcification of valve. However, effect on EndMT is not known. In addition, there evidence endothelin (ET)...
Aortic valve disease (AVD) represents a global public health challenge. Research indicates higher prevalence of diabetes in AVD patients, accelerating advancement. Although the specific mechanisms linking to dysfunction remain unclear, alterations valvular endothelial cells (VECs) homeostasis due high glucose (HG) or their crosstalk with monocytes play pivotal roles. The aim this study was determine molecular signatures VECs HG and upon interaction normal (NG) conditions propose novel...