A5071256788- Transplantation: Methods and Outcomes
- Mechanical Circulatory Support Devices
- Protease and Inhibitor Mechanisms
- Adenosine and Purinergic Signaling
- Cell Adhesion Molecules Research
- Aortic aneurysm repair treatments
- Blood Coagulation and Thrombosis Mechanisms
- Peptidase Inhibition and Analysis
- Cardiac Ischemia and Reperfusion
- Organ Transplantation Techniques and Outcomes
- Immune Cell Function and Interaction
- MicroRNA in disease regulation
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Atherosclerosis and Cardiovascular Diseases
- Tracheal and airway disorders
- Advanced Glycation End Products research
- Tissue Engineering and Regenerative Medicine
- Hydrogen's biological and therapeutic effects
- Pituitary Gland Disorders and Treatments
- Infectious Aortic and Vascular Conditions
- Reproductive System and Pregnancy
- Cardiac Structural Anomalies and Repair
- Aortic Disease and Treatment Approaches
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Electrospun Nanofibers in Biomedical Applications
University of Maryland, Baltimore
2020-2023
University of Virginia
2012-2023
University of Maryland Medical Center
2021
University of Virginia Health System
2007-2017
Landscape Research Group
2016
Biogen (United States)
2016
Indiana University – Purdue University Indianapolis
2012
Florida State University
2001-2009
Manitoba Health
2009
Armed Forces Institute of Pathology
2005
Abdominal aortic aneurysms (AAAs) are common, but their exact pathogenesis remains unknown and no specific medical therapies available. We sought to evaluate interleukin-1β (IL-1β) interleukin-1 receptor (IL-1R) in an experimental AAA model identify novel therapeutic targets for treatment.
Background— Abdominal aortic aneurysm (AAA) formation is characterized by inflammation, smooth muscle activation and matrix degradation. This study tests the hypothesis that CD4+ T-cell–produced IL-17 modulates inflammation cell activation, leading to pathogenesis of AAA human mesenchymal stem (MSC) treatment can attenuate production formation. Methods Results— Human tissue demonstrated a significant increase in IL-23 expression patients compared with control subjects as analyzed RT-PCR...
Lung ischemia-reperfusion (IR) injury after transplantation as well acute shortage of suitable donor lungs are two critical issues impacting lung transplant patients. This study investigates the anti-inflammatory and immunomodulatory role human mesenchymal stromal cells (MSCs) MSC-derived extracellular vesicles (EVs) to attenuate IR improve ex-vivo perfusion (EVLP)-mediated rehabilitation in donation circulatory death (DCD) lungs. C57BL/6 wild-type (WT) mice underwent sham surgery or using...
We recently implicated a role for CD4(+) T cells and demonstrated elevated IL-17A expression in lung ischemia-reperfusion (IR) injury. However, identification of the specific subset their mechanistic IR injury remains unknown.We tested hypothesis that invariant natural killer (iNKT) mediate via signaling.Mice underwent left hilar ligation. Pulmonary function was measured using an isolated system. Lung assessed by measuring edema (wet/dry weight) vascular permeability (Evans blue dye)....
Ischemia-reperfusion (I/R) injury (IRI), which involves inflammation, vascular permeability, and edema, remains a major challenge after lung transplantation. Pannexin-1 (Panx1) channels modulate cellular ATP release during inflammation. This study tests the hypothesis that endothelial Panx1 is key mediator of inflammation edema I/R IRI can be blocked by antagonism. A murine hilar ligation model was used whereby left lungs underwent 1 h ischemia 2 reperfusion. Treatment wild-type mice with...
Activation of invariant natural killer T (iNKT) cells and signaling through receptor for advanced glycation end products (RAGE) are known to independently mediate lung ischemia-reperfusion (IR) injury. This study tests the hypothesis that activation RAGE specifically on iNKT via alveolar macrophage-produced high mobility group box 1 (HMGB1) is critical initiation IR A murine in vivo hilar clamp model was utilized, which demonstrated RAGE(-/-) mice were significantly protected from Treatment...
Objective— B-cell depletion therapy is widely used for treatment of cancers and autoimmune diseases. B cells are abundant in abdominal aortic aneurysms (AAA); however, it unknown whether affects AAA growth. Using experimental models murine AAA, we aim to examine the effect on formation. Approach Results— Wild-type or apolipoprotein E–knockout mice were treated with mouse monoclonal anti-CD20 control antibodies subjected an elastase perfusion angiotensin II infusion model induce respectively....
Abstract Local disruption of the integrity both myoepithelial cell layer and basement membrane is an indispensable prerequisite for initiation invasion conversion human breast ductal carcinoma in situ (DCIS) to infiltrating (IDC). We previously reported that endometase/matrilysin-2/matrix metalloproteinase (MMP) 26-mediated pro-gelatinase B (MMP-9) activation promoted prostate cells by dissolving proteins (Y. G. Zhao et al., J. Biol. Chem., 278: 15056–15064, 2003). Here we report tissue...
Outcomes for lung transplantation are the worst of any solid organ, and ischemia-reperfusion injury (IRI) limits both short- long-term outcomes. Presently no therapeutic agents available to prevent IRI. Sphingosine 1-phosphate (S1P) modulates immune function through binding a set G protein-coupled receptors (S1PR1-5). Although S1P has been shown attenuate IRI, responsible protection have not defined. The present study tests hypothesis that from IRI is primarily mediated S1PR1 activation....
Rationale: Ischemia–reperfusion (IR) injury after lung transplantation, which affects both short- and long-term allograft survival, involves activation of NADPH oxidase 2 (NOX2) invariant natural killer T (iNKT) cells to produce IL-17. Adenosine A2A receptor (A2AR) agonists are known potently attenuate IR IL-17 production. However, mechanisms for iNKT cell A2AR agonist–mediated protection remain unclear.Objectives: We tested the hypothesis that NOX2 mediates production by agonism prevents...
The aim of this study was to locate sites expression and deposition collagen, fibronectin laminin in the bovine ovary. RNA from granulosa basement membrane/theca fractions maturing follicles corpora lutea early, middle late luteal phase probed with cDNAs for collagen types I IV, laminin. Antisera against collagens IV were used western analysis protein follicular fluid, granulosa, corpus luteum. Collagen subunits α1(I) α2(I) expressed but not follicle. They also all extracts, especially those...
Ex vivo lung perfusion (EVLP) enables assessment and rehabilitation of marginal donor lungs before transplantation. We previously demonstrated that adenosine A2A receptor (A2AR) agonism attenuates ischemia-reperfusion injury. The current study utilizes a novel murine EVLP model to test the hypothesis A2AR agonist enhances EVLP-mediated donation after circulatory death (DCD) lungs.Mice underwent euthanasia 60 minutes warm ischemia, were flushed with Perfadex cold static preservation (CSP,...
Recent reports of rupture in patients with abdominal aortic aneurysm (AAA) receiving B-cell depletion therapy highlight the importance understanding role B cells (B1 and B2 subsets) development AAA. We hypothesized that aggravate experimental formation. The IHC staining revealed infiltration aorta wild-type (C57BL/6) mice at day 7 after elastase perfusion persisted through 21. Quantification immune cell types using flow cytometry 14 showed significantly greater mononuclear cells, including...