A5100462000- CRISPR and Genetic Engineering
- Plant-Microbe Interactions and Immunity
- Plant Virus Research Studies
- Chromosomal and Genetic Variations
- Legume Nitrogen Fixing Symbiosis
- RNA and protein synthesis mechanisms
- Plant Molecular Biology Research
- Plant Parasitism and Resistance
- Plant Pathogenic Bacteria Studies
- Plant tissue culture and regeneration
- Plant Stress Responses and Tolerance
- Photosynthetic Processes and Mechanisms
- RNA regulation and disease
- Pancreatic and Hepatic Oncology Research
- Gastrointestinal disorders and treatments
- Pancreatitis Pathology and Treatment
- Plant responses to water stress
- Molecular Biology Techniques and Applications
- Autophagy in Disease and Therapy
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Plant nutrient uptake and metabolism
- DNA Repair Mechanisms
- Cancer-related molecular mechanisms research
- Transgenic Plants and Applications
Sun Yat-sen University
2016-2025
Ruijin Hospital
2025
Shanghai Jiao Tong University
2025
Shanghai Institute of Hematology
2025
Heilongjiang University
2024
Xinjiang Academy of Agricultural Sciences
2023-2024
The Seventh Affiliated Hospital of Sun Yat-sen University
2019-2024
Anhui Agricultural University
2023-2024
Biocon (Switzerland)
2024
Harbin Institute of Technology
2024
Plant innate immunity relies on successful detection of microbe-associated molecular patterns (MAMPs) invading microbes via pattern recognition receptors (PRRs) at the plant cell surface. Here, we report two homologous rice (Oryza sativa) lysin motif–containing proteins, LYP4 and LYP6, as dual functional PRRs sensing bacterial peptidoglycan (PGN) fungal chitin. Live imaging microsomal fractionation consistently revealed plasma membrane localization these proteins in cells. Transcription...
Plant genome sequencing has resulted in the identification of a large number uncharacterized genes. To investigate these unknown gene functions, several transient transformation systems have been developed as quick and convenient alternatives to lengthy transgenic assay. These assays include biolistic bombardment, protoplast transfection Agrobacterium-mediated transformation, each having advantages disadvantages depending on research purposes. We present novel assay based cocultivation young...
As the most accurate model for simulating light propagation in heterogeneous tissues, Monte Carlo (MC) method has been widely used field of optical molecular imaging. However, MC is time-consuming due to calculations a large number photons tissues. The structural complexity tissues further increases computational time. In this paper we present parallel implementation simulation whose surfaces are constructed by different triangle meshes. On basis graphics processing units (GPU), code...
Summary Microbe‐associated molecular pattern ( MAMP )‐triggered immunity plays critical roles in the basal resistance defense response plants. Chitin and peptidoglycan PGN ) are major patterns for fungi bacteria, respectively. Two rice Oryza sativa lysin motif‐containing proteins, Os LYP 4 6, function as receptors that sense bacterial fungal chitin. These membrane receptors, which lack intracellular kinase domains, likely contain another component transmembrane immune signal transduction....
The murine coronavirus mouse hepatitis virus (MHV) induced the expression of type I interferon (alpha/beta [IFN-alpha/beta]) in oligodendrocytic N20.1 cells. This induction is completely dependent on replication, since infection with UV light-inactivated could no longer induce IFN-alpha/beta. We show that MHV activated both transcription factors, IFN regulatory factor 3 (IRF-3) and nuclear kappaB (NF-kappaB), as evidenced by phosphorylation translocation IRF-3 an increased promoter binding...
Abstract Earlier studies have shown that RACK1 functions as a negative regulator of abscisic acid (ABA) responses in Arabidopsis (Arabidopsis thaliana), but the molecular mechanism action these processes remains elusive. Global gene expression profiling revealed approximately 40% genes affected by ABA treatment were similar manner rack1 mutation, supporting view is an important responses. On other hand, coexpression analysis more than 80% coexpressed with encode ribosome proteins, implying...
Artificial microRNA (amiRNA) approaches offer a powerful strategy for targeted gene manipulation in any plant species. However, the current unpredictability of amiRNA efficacy has limited broad application this promising technology. To address this, we developed epitope-tagged protein-based (ETPamir) screens, which target mRNAs encoding proteins were constitutively or inducibly coexpressed protoplasts with candidates targeting single multiple genes. This design allowed parallel...
Phosphatidic acid (PA) is an important lipid essential for several aspects of plant development and biotic abiotic stress responses. We previously suggested that submergence induces PA accumulation in Arabidopsis thaliana; however, the molecular mechanism underlying PA-mediated regulation submergence-induced hypoxia signaling remains unknown. Here, we showed Arabidopsis, loss phospholipase D (PLD) proteins PLDα1 PLDδ leads to hypersensitivity hypoxia, but increased tolerance submergence....
Rice is a staple food crop worldwide, and its production severely threatened by phloem-feeding insect herbivores, particularly the brown planthopper (BPH, Nilaparvata lugens), destructive pathogens. Despite identification of many BPH resistance genes, molecular basis rice to remains largely unclear. Here, we report that plant elicitor peptide (Pep) signalling confers BPH. Both PEP RECEPTORs (PEPRs) PRECURSORs (PROPEPs), OsPROPEP3, were transcriptionally induced in leaf sheaths upon...
Tomato (Solanum lycopersicum) is a globally cultivated crop with great economic value. The exocarp determines the appearance of tomato fruit and protects it from various biotic abiotic challenges at both pre-harvest post-harvest stages. However, no exocarp-specific promoter currently available, which hinders exocarp-based genetic engineering. Here, we identified by RNA sequencing reverse transcription-quantitative PCR analyses that gene SlPR10 (PATHOGENESIS RELATED 10) was abundantly...
Abstract Background. The EACH study assessed the efficacy of oxaliplatin, 5-fluorouracil, and leucovorin (the FOLFOX4 regimen) compared with doxorubicin alone in terms overall survival (OS), progression-free (PFS), safety patients advanced hepatocellular carcinoma (HCC). We present results this Chinese patients. Methods. In a multicenter, open-label, randomized, phase III (00471965), 371 (279 from People's Republic China) were randomized 1:1 to receive either or until disease progression,...
In plants, the ubiquitin-proteasome system, endosomal sorting, and autophagy are essential for protein degradation; however, their interplay remains poorly understood. Here, we show that four Arabidopsis (Arabidopsis thaliana) E3 ubiquitin ligases, SEVEN IN ABSENTIA OF ARABIDOPSIS THALIANA1 (SINAT1), SINAT2, SINAT3, SINAT4, regulate stabilities of FYVE DOMAIN PROTEIN REQUIRED FOR ENDOSOMAL SORTING1 (FREE1) VACUOLAR SORTING23A (VPS23A), key components sorting complex required transport-I, to...
CRISPR/Cas-derived base editing tools empower efficient alteration of genomic cytosines or adenines associated with essential genetic traits in plants and animals. Diversified target sequences customized products call for editors distinct features regarding the window scope. Here we developed a toolkit plant containing AID10, an engineered human AID cytosine deaminase. When fused to N-terminus C-terminus conventional Cas9 nickase (nSpCas9), AID10 exhibited broad narrow activity at...
Abstract CD8+CD28− human T suppressor cells (Ts) act on APC, inhibiting their ability to elicit Th activation and proliferation. This effect is due inhibition of the CD40 pathway which normally leads CD80 CD86 up-regulation. To determine whether Ts inhibit expression B7 molecules by blocking transcription, we cloned characterized promoter. Mutational analysis revealed that transcription driven The NF-κB binding site, at −612 promoter, essential for Th-induced transcription. In cultures...