A5049651692- interferon and immune responses
- Viral Infections and Vectors
- Hematopoietic Stem Cell Transplantation
- RNA modifications and cancer
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Mosquito-borne diseases and control
- Autoimmune and Inflammatory Disorders Research
- Cytomegalovirus and herpesvirus research
- CAR-T cell therapy research
- Single-cell and spatial transcriptomics
- Immunotherapy and Immune Responses
- Acute Lymphoblastic Leukemia research
- Acute Myeloid Leukemia Research
- Immune cells in cancer
- Viral Infections and Outbreaks Research
- Platelet Disorders and Treatments
- Genomics and Chromatin Dynamics
- Advanced biosensing and bioanalysis techniques
- Circadian rhythm and melatonin
- Immune responses and vaccinations
- Free Radicals and Antioxidants
- Diabetes and associated disorders
- Inflammasome and immune disorders
- Cancer-related molecular mechanisms research
Technical University of Munich
2022-2025
München Klinik Schwabing
2024
TU Dresden
2016-2023
University Hospital Carl Gustav Carus
2021
Institute of Immunology
2016-2019
German University in Cairo
2014
Abstract Hematopoietic stem cells (HSCs) produce highly diverse cell lineages. Here, we chart native lineage pathways emanating from HSCs and define their physiological regulation by computationally integrating experimental approaches for fate mapping, mitotic tracking, single-cell RNA sequencing. We find that lineages begin to split when leave the tip HSC population, marked high Sca-1 CD201 expression. Downstream, either retain expression ability generate lymphocytes, or irreversibly reduce...
Blood cell generation depends on continuous cellular output by the sequential hierarchy of hematopoietic stem (HSC) and progenitor populations that all contain quiescent actively cycling cells. Hematopoietic cells (HSPCs) express surface molecule Stem antigen 1 (SCA-1/LY6A). Using histone 2B-red fluorescent fusion protein label retention cell-cycle reporter mice, we demonstrate high SCA-1 expression (SCA-1hi) identifies not only HSCs but hierarchical levels within lineage−SCA-1+KIT+ (LSK)...
Abstract Viral infections pose a significant global burden. Host susceptibility to pathogens is determined by many factors including genetic variation that can lead immunodeficient or dysregulated antiviral immune responses. Pax5 heterozygosity ( −/+ ), resulting in reduced PAX5 levels mice, mimics germline somatic dysregulation contributing diseases such as childhood B-cell precursor acute lymphoblastic leukemia (B-ALL). In contrast the well-characterized roles of during early development,...
The proliferative activity of aging hematopoietic stem cells (HSCs) is controversially discussed. Inducible fluorescent histone 2B fusion protein (H2B-FP) transgenic mice are important tools for tracking the mitotic history murine HSCs in label dilution experiments. A recent study proposed that primitive symmetrically divide only four times to then enter permanent quiescence. We observed background fluorescence due leaky H2B-FP expression, occurring all transgenes independent induction,...
Genome damage is a main driver of malignant transformation, but it also induces aberrant inflammation via the cGAS/STING DNA-sensing pathway. Activation can trigger cell death and senescence, thereby potentially eliminating genome-damaged cells preventing against transformation. Here, we report that defective ribonucleotide excision repair (RER) in hematopoietic system caused genome instability with concomitant activation axis compromised stem function, ultimately resulting leukemogenesis....
A novel series of dimeric melatonin analogues obtained by connecting two molecules through N1 with spacers 15–24 atoms was synthesized and characterized in 2-[125-I]-iodomelatonin binding bioluminescence resonance energy transfer (BRET) experiments at MT1 MT2 receptors. Compounds 4 (16 spacer) 13 (24 are among the ligands inducing maximal BRET MT2-homodimers as well both types MT1/MT2 heterodimers. Notably, ligand-induced changes observed for compounds linked 22–24 could be attributed to...
Somatic loss of function mutations in cohesin genes are frequently associated with various cancer types, while disruption the germline causes cohesinopathies such as Cornelia-de-Lange syndrome (CdLS). Here, we present discovery a recurrent heterozygous RAD21 aberration at amino acid position 298 (p.P298S/A) identified three children lymphoblastic leukemia or lymphoma total dataset 482 pediatric patients. While p.P298S/A did not disrupt formation complex, it altered gene expression, DNA...
Introduction Pediatric sarcomas, including osteosarcoma (OS), Ewing sarcoma (EwS) and rhabdomyosarcoma (RMS) carry low somatic mutational burden MHC-I expression, posing a challenge for T cell therapies. Our previous study showed that mediators of monocyte maturation sensitized the EwS line A673 to lysis by HLA-A*02:01/CHM1 319 -specific allorestricted receptor (TCR) transgenic CD8 + cells (CHM1 cells). Methods In this study, we tested panel cytokines their ability upregulate immunogenic...
<p>Oligo nucleotide sequences</p>
<p>Supplementary Figures S1-8 and corresponding figure legends</p>
<p>List of antibodies used in the study</p>
<p>Supplementary Figures S1-8 and corresponding figure legends</p>
<p>Oligo nucleotide sequences</p>
<p>List of antibodies used in the study</p>
<div>Abstract<p>Genome damage is a main driver of malignant transformation, but it also induces aberrant inflammation via the cGAS/STING DNA sensing pathway. Activation can trigger cell death and senescence, thereby potentially eliminating genome-damaged cells preventing against transformation. Here, we report that defective ribonucleotide excision repair (RER) in hematopoietic system caused genome instability with concomitant activation axis compromised stem function, ultimately...
Abstract The proliferative activity of adult hematopoietic stem cells (HSCs) is controversially discussed. Inducible fluorescent histone 2B fusion protein (H2B-FP) transgenic mice are important tools for tracking the mitotic history murine HSCs in label dilution experiments. A recent study proposed that most primitive divide only four times, to then enter permanent quiescence. We observed background fluorescence due leaky H2B-FP expression, occurring all transgenes independent induction,...
Abstract Hematopoietic stem cells (HSCs) produce a highly diverse array of cell lineages. To assay hematopoietic differentiation with minimal experimental perturbation, non-invasive methods for heritable labeling 1–3 or barcoding 4–7 HSCs in vivo have recently been developed and used to study lineage fate physiological conditions. However, the pathways leading from mature remain controversial 8 , suggested models ranging gradual restriction branching cascade progenitors already making...