A5101787089- Animal Genetics and Reproduction
- CRISPR and Genetic Engineering
- Virus-based gene therapy research
- Nanoparticles: synthesis and applications
- Graphene and Nanomaterials Applications
- MicroRNA in disease regulation
- Reproductive Biology and Fertility
- Metabolism and Genetic Disorders
- Folate and B Vitamins Research
- Mosquito-borne diseases and control
- Circular RNAs in diseases
- Insect symbiosis and bacterial influences
- Plant Virus Research Studies
- Sirtuins and Resveratrol in Medicine
- Infant Nutrition and Health
- RNA Interference and Gene Delivery
- Reproductive System and Pregnancy
- Xenotransplantation and immune response
- Histone Deacetylase Inhibitors Research
- RNA modifications and cancer
- Nanoparticle-Based Drug Delivery
- Muscle Physiology and Disorders
- Nanoplatforms for cancer theranostics
- Selenium in Biological Systems
- Carbon and Quantum Dots Applications
Yangzhou University
2015-2025
Colorado Center for Reproductive Medicine
2022
Konkuk University
2017-2020
Jiangsu Provincial Center for Disease Control and Prevention
2020
Gyeongsang National University
2018-2020
Kerala Veterinary and Animal Sciences University
2017
Silver nanoparticles (AgNPs) are widely used metal in health care industries, particularly due to its unique physical, chemical, optical, and biological properties. It is as an antibacterial, antiviral, antifungal, anticancer agent. Camptothecin (CPT) derivatives function inhibitors of topoisomerase potent agents against a variety cancers. Nevertheless, the combined actions CPT AgNPs apoptosis human cervical cancer cells (HeLa) have not been elucidated. Hence, we investigated synergistic...
Background: Gemcitabine (GEM) is widely used as an anticancer agent in several types of solid tumors. Silver nanoparticles (AgNPs) possess unique cytotoxic features and can induce apoptosis a variety cancer cells. In this study, we investigated whether the combination GEM AgNPs exert synergistic effects human ovarian cell line A2780. Methods: We synthesized using resveratrol reducing stabilizing agent. The nanomaterials were characterized various analytical techniques. combined treatment...
Background: Cisplatin (Cis) is a widely used chemotherapeutic drug for treating variety of cancers, due to its ability induce cell death in cancer cells significantly. Recently, graphene and modified nanocomposites have gained much interest therapy, their unique physicochemical properties. The objective this study was investigate the combination effect Cis reduced oxide–silver nanoparticle nanocomposite (rGO-AgNPs) human cervical (HeLa) cells. Materials methods: We synthesized AgNPs, rGO,...
Graphene and graphene-related materials have gained substantial interest from both academia industry for the development of unique nanomaterials biomedical applications. oxide (GO) silver nanoparticles (AgNPs) are a valuable platform nanocomposites, permitting combination with different physical chemical properties to generate novel improved effective functionalities in single platform. Therefore, this study was conducted synthesize graphene oxide-silver nanoparticle (GO-AgNPs) nanocomposite...
The translation of global cellular proteins is almost completely repressed in cells with flavivirus infection, while viral remains efficient. mechanisms flaviviruses evade host translational shutoff are largely unknown. Here, we identified elements and factors associated JEV evasion shutoff. 5′UTR lacked IRES or IRES-like activity, noncapped initiated the presence 3′UTR. Furthermore, DB2 sHP-SL within 3′UTR were involved regulation cap-independent translation, which conserved genus...
The translation of global cellular proteins is almost completely repressed in cells with flavivirus infection, while viral remains efficient. mechanisms flaviviruses evade host translational shutoff are largely unknown. Here, we identified elements and factors associated JEV evasion shutoff. 5′UTR lacked IRES or IRES-like activity, noncapped initiated the presence 3′UTR. Furthermore, DB2 sHP-SL within 3′UTR were involved regulation cap-independent translation, which conserved genus...
Myostatin (MSTN) protein, lncRNAs, and circRNAs regulate skeletal muscle growth development. This work aims to compare the expression patterns of lncRNAs in gluteus maximus tissue wild-type (WT) MSTN gene knockout (KO) rabbits. Within three WT four KO rabbits, we analyzed profiles lncRNAs. After identifying differently expressed RNAs, biological pathways implicated were ascertained by performing enrichment analysis using Kyoto Encyclopedia Genes Genomes (KEGG) Gene Ontology (GO). We...
Fel d1 is the most important allergen secreted by cats, which can trigger asthma in sensitive individuals. Our objective was to knock-out gene fetal fibroblasts of cats through CRISPR-Cas9 technology with two sgRNAs and determine impact such mutations on antigenicity protein. DNA samples from 38 domestic were collected amplified PCR obtain complete sequence gene. Throughout evolution, polypeptide chain 1(CH1) has proven be much more conserved than 2(CH2); therefore, we targeted CH2 designed...
Rabbits (Oryctolagus cuniculus) have been the very frequently used as animal models in study of human lipid metabolism and atherosclerosis, because they similar lipoprotein to humans. Most hyperlipidemia atherosclerosis rabbit are produced by feeding rabbits a high-cholesterol diet. Gene editing or knockout (KO) offered another means producing for lipids lipoproteins. Even so, apolipoprotein (Apo)E KO must be fed diet induce hyperlipidemia. In this study, we CRISPR/Cas9 system anchored exon...
Background: Breast cancer is the most common malignant disease that occurs in women. Histone deacetylase (HDAC) inhibition has recently emerged as an effective and attractive target for treatment of cancer. The aim this study was to investigate efficacy a combined tubastatin A (TUB-A) palladium nanoparticles (PdNPs) against MDA-MB-231 human breast cells using two different cytotoxic agents work by mechanisms, thereby decreasing probability chemoresistance increasing toxicity, provide...
Myostatin (MSTN) encodes a negative regulator of skeletal muscle mass that might have applications for promoting growth in livestock. In this study, we aimed to test whether targeted MSTN editing, mediated by transcription activator-like effector nucleases (TALENs), is viable approach create myostatin-modified goats (Capra hircus).We obtained pair TALENs (MTAL-2) could recognize and cut the site goat genome. Fibroblasts from pedigreed were co-transfected with MTAL-2, 272 monoclonal cell...
Graphene oxide–silver nanoparticle (GO-AgNPs) nanocomposites have drawn much attention for their potential in biomedical uses. However, the toxicity of GO-AgNPs animals and humans remains unknown, particularly developing fetus. Here, we reported GO-AgNP-mediated cytotoxicity epigenetic alteration status caprine fetal fibroblast cells (CFFCs). In brief, proliferation apoptosis rate GO-AgNP-treated CFFCs (4 8 µg/mL GO-AgNPs) were measured using cell-counting kit (CCK-8) assay annexin...
Many diseases, including myocardial infarction, autoimmune disease, viral neurodegenerative and cancers, are frequently diagnosed with aberrant expression of microRNAs (miRNAs) their allied pathways. This indicates the crucial role miRNAs in maintaining biological physiological processes. miR-7641 is a miRNA whose disease has not been fully investigated. In present study, we investigated pattern its target genes different cancer cells, as well clinical patients. Our data confirmed RPS16...
The increasing industrial and biomedical utilization of graphene oxide silver nanoparticles (GO-AgNPs) raises the concern nanosafety: exposure to AgNPs or GO-AgNPs increases generation reactive oxygen species (ROS), causes DNA damage alters expression whole transcriptome including mRNA, miRNA, tRNA, lncRNA, circRNA others. Although roles different RNAs in epigenetic toxicity are being studied during last decade, but still we have little knowledge about role circle (circRNAs) toxicity.Rabbit...
It is very important to develop an effective, specific, and robust expression cassette that ensures a high level of in the mammary glands. In this study, we designed constructed series gland-specific vectors containing complex hybrid promoter/enhancer by utilizing promoter sequences from milk proteins (i.e., goat β-casein, bovine αs1-casein, or β-lactoglobulin) cytomegalovirus enhancer sequences; single protein served as controls. Chicken β-globin insulator were also included some these...
Poor expression is the key factor hampering large-scale application of transgenic animal mammary gland bioreactors. A very different approach would be to evaluate secretion recombinant proteins into milk in response a cleavable signal peptide highly secreted lactoproteins.We previously reported rabbits harboring gland-specific vector containing fusion cDNA (goat β-lactoglobulin (BLG) and human plasminogen activator (rhPA) coding sequences) expressed rhPA milk, but we did not realize...
To improve nutrient content of goat milk, we describe the construction a vector (pBLAC) containing hybrid β -lactoglobulin (BLG) promoter/cytomegalovirus (CMV) enhancer. We also generation transgenic goats expressing rhLA by somatic cell nuclear transfer (SCNT). Of 334 one-cell stage embryos derived from three lines and 99 non-transgenic (NT) cells surgically transferred to oviducts 37 recipients, two recipients delivered kids (2%) non-transfected line five six (1.8%) lines, which died...