A5052873526- Reproductive Biology and Fertility
- Pluripotent Stem Cells Research
- Animal Genetics and Reproduction
- Ovarian function and disorders
- Protein Tyrosine Phosphatases
- Tissue Engineering and Regenerative Medicine
- CRISPR and Genetic Engineering
- Telomeres, Telomerase, and Senescence
- Plant tissue culture and regeneration
- Sirtuins and Resveratrol in Medicine
- Allergic Rhinitis and Sensitization
- Reproductive System and Pregnancy
- Bioactive natural compounds
- vaccines and immunoinformatics approaches
- Renal and related cancers
- MicroRNA in disease regulation
- Phytase and its Applications
- Genomics, phytochemicals, and oxidative stress
- Medicinal plant effects and applications
- Adenosine and Purinergic Signaling
- Ginger and Zingiberaceae research
- Autophagy in Disease and Therapy
- Viral Infectious Diseases and Gene Expression in Insects
- Lipid metabolism and biosynthesis
- Hemoglobinopathies and Related Disorders
Gyeongsang National University
2019-2025
University of Swat
2018-2022
Khyber Medical College
2014-2022
Ayub Medical College
2021
Jilin Agricultural University
2020
Osun State University
2020
Exposure to reactive oxygen species (ROS) leads the oxidation of low-density lipoproteins (LDL), converting them into oxidized ones (oxLDL), which are involved in pathogenesis Alzheimer's disease, suggesting a potential link between lipid dysregulation and neurodegenerative processes. Phenolic metabolites derived from Artocarpus elasticus root bark were found possess significant antioxidant properties at three different radical scavenging assays, including 2,2-diphenyl-1-picrylhydrazyl...
Abstract Allergens from domestic cats ( Felis catus ) cause allergy-related health problems worldwide. Fel d 1 is a major allergen that causes severe allergic reactions in humans, including rhinitis, conjunctivitis, and life-threatening asthma. Therefore, patients with cat allergies anticipate hypoallergenic cats. We successfully generated chain 2 (CH2) genome-edited using the CRISPR-Cas9 system this study. T7 endonuclease assay Sanger sequencing were used to confirm mutation CH2 level...
Melatonin, a nighttime-secreted antioxidant hormone produced by the pineal gland, and AKT, serine/threonine-specific protein kinase, have been identified as regulators for several cellular processes essential reproduction. The current study aimed to investigate potential interplay between melatonin AKT in bovine oocytes context of embryo development. Results showed that inclusion SH6, specific inhibitor, during vitro maturation (IVM) significantly reduced oocyte maturation, cumulus cell...
Abstract We propose an effective surface plasmon resonance system designed to achieve both negative and positive Goos-Hanchen shifts in reflected light. This comprises a metal film underlying medium, where the real part of permittivity medium must be less than unity. Surface polaritons can excited at lower when light is incident from air onto upper metal. The excitation leads exploration shift (G-HS). Control over (G-HS) investigated via wavelength magnitude G-HS strongly dependent on...
We have previously reported that juglone, a natural compound found in Juglandaceae with wide range of biological activities, can reduces the developmental competence bovine oocytes. In current study, we investigated possible mechanisms behind toxicity juglone and relationship PI3K/AKT/mTOR signaling during vitro maturation (IVM) Results show oocyte exposure to was associated significant decrease filamentous actin (F-actin) accumulation. The RT-qPCR showed downregulation meiosis progression...
Transforming growth factor-beta (TGF-β) plays a critical role in regulating trophoblast invasion and proliferation. Growth differentiation factor-8 (GDF-8) is member of the TGF-β superfamily categorized as myostatin subtype. It primarily secreted protein synthesized skeletal muscle cells. expressed placenta, reproductive tissues, In this study, we investigated GDF-8 development hatching rate bovine embryos. We noted notable elevation ( p < 0.05) rates compared to control Furthermore,...
Sex-related growth differences between male and female embryos remain an attractive subject for reproductive biologists. This study aimed to investigate the endogenous factors that play a crucial role in pace of early development bovine embryos. Using sex pre-selected semen by Y-specific monoclonal antibodies production embryos, we characterized critical are responsible creating differences, especially during pre-implantation period Our results showed at day seven, (57.8%) Y-sperm sorted...
This study was aimed to investigate the role of SHP2 (Src-homology-2-containing phosphotyrosine phosphatase) in intricate signaling networks invoked by bovine oocyte achieve maturation and blastocyst development. PTPN11 (Protein Tyrosine Phosphatase, non-receptor type 11) encoding protein SHP2, a positive transducer RTKs (Receptor Kinases) cytokine receptors, can play significant embryo development, but this phenomenon has not yet been explored. Here, we used different growth factors,...
The PPARs (peroxisome proliferator-activated receptors) play critical roles in the regulation of lipid and glucose metabolism. PPARδ, a member family, is associated with decreased susceptibility to ectopic deposition implicated mitochondrial processes. current study aimed determine role PPARδ fatty acid β-oxidation its influence on PEPCK for lipogenic/lipolytic balance during vitro bovine oocyte maturation embryo development. Activation by GW501516, but not 2-BP, was indicated intact...
Nicotinamide (NAM), the amide form of vitamin B3, plays pivotal roles in regulating various cellular processes including energy production and maintenance genomic stability. The current study aimed at deciphering effect NAM, when administered during vitro maturation (IVM), on developmental competence bovine preimplantation embryos. Our results showed that low NAM concentrations reduced oxidative stress improved mitochondrial profile, total cleavage 8–16 cell stage embryo development whereas...
Src-homology-2-containing phosphotyrosine phosphatase (SHP2), a classic cytoplasmic protein and major regulator of receptor tyrosine kinases G protein-coupled receptors, plays significant role in preimplantation embryo development. In this study, we deciphered the SHP2 somatic compartment oocytes during meiotic maturation. showed nuclear/cytoplasmic localization bovine cumulus human granulosa (COV434) cells. Follicle-stimulating hormone (FSH) treatment significantly enhanced localization,...
Wnt/β-catenin signaling plays vital role in the regulation of cellular proliferation, migration, stem cells cell renewal and genetic stability. This pathway is crucial during early developmental process; however, distinct pre-implantation period bovine embryonic development obscure. Here, we evaluated critical blastocyst (BL) hatching. 6 bromoindurbin-3’oxime (6-Bio) was used to stimulate Wnt signaling. Treatment with 6-Bio induced expression peroxisome proliferator-activated receptor-delta...
Exosomes are nano-sized vesicles with abundant nucleic acids, proteins, lipids, and other regulatory molecules. The aim of this study was to examine the effect BOEC–Exo on bovine in vitro oocyte maturation embryo development. We found that a 3% Exo supplementation IVM media significantly enhanced reduced accumulation ROS MII-stage oocytes. Oocyte related genes (GDF9 CPEB1) also confirmed treatment oocytes (p < 0.05) maturation. Next, we cultured cumulus cells assessed effects Exo, which...
Age-associated decline in oocyte quality is one of the dominant factors low fertility. Aging alters several key processes, such as telomere lengthening, cell senescence, and cellular longevity granulosa cells surrounding oocyte. To investigate age-dependent molecular changes, we examined expression, localization, correlation telomerase reverse transcriptase (TERT) β-Klotho (KLB) bovine cells, oocytes, early embryos during aging process. Herein, cumulus-oocyte complexes (COCs) obtained from...
is a ubiquitous Gram-negative emerging pathogen that causes hospital-acquired infection in both immunocompromised and immunocompetent patients. It multi-drug-resistant bacterium; therefore, an effective subunit immunogenic candidate of great interest to encounter the pathogenesis this pathogen. A protein-wide annotation targets was performed fast-track vaccine development against pathogen, structural-vaccinology-assisted epitopes were predicted. Among total proteins, only three, A0A1T3FLU2,...
Age-related deterioration in the reproductive capacity of women is directly related to poor developmental potential ovarian follicles. Although telomerase plays a key role female fertility, TERT-targeting therapeutic strategies for age-related infertility have yet be investigated. This study elucidated effect Telomerase activation on mice ovaries and more specifically Klb (β-Klotho) gene expression, which linked ageing, hormonal regulation, cyclicity. The homology-based 3D model hTERT was...
Embryonic genome activation (EGA) is a critical step during embryonic development. Several transcription factors have been identified that play major roles in initiating EGA; however, this gradual and complex mechanism still needs to be explored. In study, we investigated the role of nuclear factor Y subunit A (NFYA) bovine EGA development its relationship with platelet-derived growth receptor-β (PDGFRβ) by using potent selective activator (PDGF-BB) inhibitor (CP-673451) PDGF receptors....