This study investigated the use of bovine serum albumin (BSA) plus insulin–transferrin–sodium selenite (ITS) and/or epidermal growth factor (EGF) as alternatives to fetal (FBS) in embryo culture medium. The developmental ability and quality embryos were determined by assessing their cell number, lipid content, gene expression cryotolerance, well invasion trophoblasts. percentage that underwent cleavage formed a blastocyst was higher (P<0.01) medium containing ITS EGF BSA than FBS....

Melatonin, a nighttime-secreted antioxidant hormone produced by the pineal gland, and AKT, serine/threonine-specific protein kinase, have been identified as regulators for several cellular processes essential reproduction. The current study aimed to investigate potential interplay between melatonin AKT in bovine oocytes context of embryo development. Results showed that inclusion SH6, specific inhibitor, during vitro maturation (IVM) significantly reduced oocyte maturation, cumulus cell...

Graphene oxide–silver nanoparticle (GO-AgNPs) nanocomposites have drawn much attention for their potential in biomedical uses. However, the toxicity of GO-AgNPs animals and humans remains unknown, particularly developing fetus. Here, we reported GO-AgNP-mediated cytotoxicity epigenetic alteration status caprine fetal fibroblast cells (CFFCs). In brief, proliferation apoptosis rate GO-AgNP-treated CFFCs (4 8 µg/mL GO-AgNPs) were measured using cell-counting kit (CCK-8) assay annexin...

Acrylamide (ACR) and its metabolite glycidamide (GLY) are contaminants with known toxic effects, especially in reproductive systems. However, the mechanisms underlying their embryotoxic effects remain inadequately understood. In current study, we investigated of ACR GLY exposure on oocyte embryo developmental competence, focusing DNA damage, apoptosis, autophagy, epigenetic regulation. Oocytes were exposed to varying concentrations during vitro maturation. The results demonstrated that both...

We have previously reported that juglone, a natural compound found in Juglandaceae with wide range of biological activities, can reduces the developmental competence bovine oocytes. In current study, we investigated possible mechanisms behind toxicity juglone and relationship PI3K/AKT/mTOR signaling during vitro maturation (IVM) Results show oocyte exposure to was associated significant decrease filamentous actin (F-actin) accumulation. The RT-qPCR showed downregulation meiosis progression...

Lameness is a significant problem for the dairy industry worldwide. No previous studies have evaluated prevalence of lameness or digital dermatitis (DD) in cattle herds Egypt. A total 16,098 cows from 55 11 Egyptian governorates underwent visual locomotion scoring using 4-point system. Cows that had score ≥ 2 were considered clinically lame. Following manure removal with water and flashlight, cows' hind feet examined milking parlour to identify DD lesions classify M-score. Furthermore, each...

The aim of the present study was to investigate beneficial effect polydatin (PD), glycoside form resveratrol, on embryo development in vitro. Oocytes were aspirated from ovaries Korean Hanwoo cows and cultured until Day 8 a humidified atmosphere 5% CO2 air at 38.5°C. Protein gene expression levels determined through confocal microscopy reverse transcription–polymerase chain reaction respectively, whereas number total apoptotic cells blastocysts using Hoechst 33342 staining terminal...

Sex preselection has always generated great interest among livestock producers. Among the prevalent sperm sorting methods, there is much evidence that sex a negative effect on quality with an altered pattern of motility, ultimately reducing period cell viability. In this study, we have established new approach for preselected embryo production by using WholeMom®; monoclonal antibody developed against bull epitopes simple and easy separation X- Y-sperm. There were no significant differences...

In vitro embryo development remains suboptimal compared to in vivo due the challenge from various stressors associated with culturing of oocytes. When 0.2 μM lycopene was added oocyte maturation and culture media, assess its antioxidant effects on development, we observed a significant ( p < 0.05) increase cleavage blastocyst rates corresponding controls (84.3 ± 0.6% vs. 73.1 1.9% 41.0 1.4% 33.4 0.7%, respectively). Lycopene also significantly reduced intracellular reactive oxygen species...

Heat stress has large effects on reproduction including conception rate in cattle. In this study, we examined the of coagulansin-A (coa-A), a steroidal lactone, acquired thermo tolerance during vitro production bovine embryos. Oocytes were incubated maturation (IVM) media with or without coa-A at two different temperatures, 40.5˚C and 42˚C, for 20 h. The treatment significantly improved blastocyst development only (P < 0.05). Interestingly, immunofluorescence analysis demonstrated that...

Somatic cell nuclear transfer (SCNT) is a useful technology; however, its efficiency low. In this study, we investigated the effects of cytoplasmic into enucleated oocytes on developmental competence and quality cloned preimplantation bovine embryos via terminal deoxynucleotidyl transferase dUTP nick-end labeling, quantitative reverse transcription PCR, immunocytochemistry. We used cytoplasm injection cloning technology (CICT), new technique which volume an oocyte could be restored by...

Nicotinamide (NAM), the amide form of vitamin B3, plays pivotal roles in regulating various cellular processes including energy production and maintenance genomic stability. The current study aimed at deciphering effect NAM, when administered during vitro maturation (IVM), on developmental competence bovine preimplantation embryos. Our results showed that low NAM concentrations reduced oxidative stress improved mitochondrial profile, total cleavage 8–16 cell stage embryo development whereas...

Juglone, a major naphthalenedione component of walnut trees, has long been used in traditional medicine as an antimicrobial and antitumor agent. Nonetheless, its impact on oocyte preimplantation embryo development not entirely clarified. Using the bovine model, we sought to elucidate juglone treatment during vitro maturation (IVM) oocytes their embryos. Results showed severe reduction nuclear cumulus expansion significant increase mitochondrial dysfunction reactive oxygen species (ROS)...

Vanillic acid (VA) has shown antioxidant and anti-inflammatory activities in different cell types, but its biological effects the context of early embryo development have not yet been clarified. In current study, impact VA supplementation during vitro maturation (IVM) and/or post-fertilization (in culture; IVC) on redox homeostasis, mitochondrial function, AKT signaling, developmental competence, quality bovine pre-implantation embryos was investigated. The results showed that dual exposure...

This study was designed to investigate the effect of different concentrations L-cysteine supplementation into maturation medium on oocyte nuclear maturation, cumulus cell expansion, ultrastructure oocytes and expression oocyte-derived growth factors BMP-15, GDF-9 CB-1 genes. Cumulus complexes (COCs) were collected from cow's ovaries obtained abattoir incubated at 38.5°C in media supplemented with 0, 0.6, 0.8 or 1 mM 5% CO2 under humidified air for 24 hr. We found that a significantly higher...

Abstract Increased oxidative stress is one of the main causes poorly developed embryos in assisted reproductive technologies. Nicotinamide (NAM) has been shown to suppress reactive oxygen species (ROS) production through its potent antioxidative and anti‐senescent effects. In present study, we explored effects short‐term NAM‐treatment (3 5 h) during vitro fertilization (IVF) on development bovine embryos. Treatment with 10 mM NAM for 3 h significantly increased blastocyst formation but...