A5059691983- Liver Disease Diagnosis and Treatment
- Liver physiology and pathology
- Liver Disease and Transplantation
- Endoplasmic Reticulum Stress and Disease
- Pancreatic function and diabetes
- Drug Transport and Resistance Mechanisms
- FOXO transcription factor regulation
- Organ Transplantation Techniques and Outcomes
- Genomics, phytochemicals, and oxidative stress
- Hepatocellular Carcinoma Treatment and Prognosis
- Lipid metabolism and disorders
- Cytokine Signaling Pathways and Interactions
- Polyamine Metabolism and Applications
- Lipid metabolism and biosynthesis
- Neuropeptides and Animal Physiology
- Macrophage Migration Inhibitory Factor
- Pediatric Hepatobiliary Diseases and Treatments
- Cholesterol and Lipid Metabolism
- Antimicrobial Peptides and Activities
- Diet, Metabolism, and Disease
- Cannabis and Cannabinoid Research
- Heat shock proteins research
- Growth Hormone and Insulin-like Growth Factors
- Pediatric health and respiratory diseases
- Amino Acid Enzymes and Metabolism
University Hospital Regensburg
2011-2024
University of Regensburg
2004-2018
Damascus University
2017-2018
Uniwersytecki Szpital Dziecięcy
2018
Klinik und Poliklinik für Kinder- und Jugendmedizin
2011
Abstract Liver regeneration can be impaired by permanent oxidative stress and activation of nuclear factor erythroid 2-related 2 (Nrf2), known to regulate the cellular antioxidant response, has been shown improve process liver regeneration. A variety factors hepatic tissue regeneration, among them augmenter (ALR), attained great attention as being survival for with proproliferative antiapoptotic properties. Here we determined Nrf2/ response element (ARE) regulated expression ALR show a...
Given the important role of angiogenesis in liver pathology, current study investigated Runt-related transcription factor 1 (RUNX1), a regulator developmental angiogenesis, pathogenesis non-alcoholic steatohepatitis (NASH). Quantitative RT-PCRs and analysis angiogenesis-associated differentially expressed genes tissues healthy controls, patients with steatosis NASH, indicated potential RUNX1 NASH. The gene expression was correlated histopathological attributes patients. protein studied by...
The ATP-binding cassette transporter A1 (ABCA1) facilitates the cellular release of cholesterol and choline-phospholipids to apolipoprotein A-I (apoA-I) several studies indicate that vesicular transport is associated with ABCA1 function. Syntaxins play a major role in fusion have also been demonstrated interact members ABC-transporter family. Therefore, we focused on identification syntaxins directly ABCA1. expression cultured human monocytes during M-CSF differentiation loading was...
Augmenter of liver regeneration (ALR), which is critically important in and hepatocyte proliferation, highly expressed cirrhotic livers hepatocellular carcinomas (HCC). In the current study, functional role ALR hepatocancerogenesis was analyzed more detail. HepG2 cells, cytosolic 15 kDa isoform reexpressed stably, (HepG2-ALR) were used migration invasion assays using modified Boyden chambers. Epithelial-mesenchymal transition (EMT) markers determined HepG2-ALR cells vitro tumors grown nude...
Nonalcoholic fatty liver disease (NAFLD) covers a spectrum from simple steatosis to nonalcoholic steatohepatitis (NASH) and cirrhosis. Free acids (FFA) induce lipo-toxicity correlate with severity of NAFLD. In this study we aimed investigate the role exogenous endogenous ALR (augmenter regeneration) for FFA induced ER (endoplasmatic reticulum) -stress lipoapoptosis. Primary human hepatocytes or hepatoma cells either treated recombinant (rhALR, 15kDa) expressing short form (sfALR, were...
Metabolic dysfunction-associated steatotic liver disease (MASLD) comprises a spectrum of diseases, ranging from steatosis to metabolic steatohepatitis (MASH), increasing the risk developing cirrhosis and hepatocellular carcinoma (HCC). Fibrosis within MASLD is critical for development; therefore, identification fibrosis-driving factors indispensable. We analyzed expression interleukin 32 (IL-32) chemokine CC ligand 20 (CCL20), which are known be linked with inflammation fibrosis, their in...
Pathological disorders of the liver were shown to be associated with an impairment hepatic drug metabolism mediated in part by growth factors. Augmenter regeneration (ALR) is a novel liver-specific hepatotrophic factor, whereas its action on cytochrome P450 (P450) completely unknown. Application ALR primary human hepatocytes vitro reduced isoenzyme activities (1A2 and 2A6) dose-dependent manner. Time-course analysis revealed that maximal inhibitory effect was reached after 24 72 h exposure...
Background & Aims: Knowledge about innate antimicrobial defense of the liver is limited. We investigated hepatic expression and regulation peptides with focus on human beta defensin-1 (hBD-1). Methods: Radial diffusion assay was used to analyze activity tissue. Different defensins including hBD-1 its activator thioredoxin-1 (TXN) were analyzed in healthy cholestatic samples by qPCR immunostaining. Regulation studied vitro vivo using bile duct ligated mice. via bilirubin acids (BAs) siRNA....
Hepatic ischemia reperfusion injury (IRI) is a major complication in liver resection and transplantation. Here, we analyzed the impact of recombinant human augmenter regeneration (rALR), an anti-oxidative anti-apoptotic protein, on deleterious process induced by (IR). Application rALR reduced tissue damage (necrosis), levels lipid peroxidation (oxidative stress) expression genes mouse IRI model. Damage associated molecule pattern (DAMP) inflammatory cytokines such as HMGB1 TNFα, were not...
Inflammasomes and innate immune cells have been shown to contribute liver injury, thereby activating Kupffer cells, which release several cytokines, including IL-6, IL-1ß, TNFα. Augmenter of regeneration (ALR) is a hepatotropic co-mitogen that was found anti-oxidative anti-apoptotic properties attenuate experimental non-alcoholic fatty disease (NAFLD) cholestasis. Additionally, hepatic ALR expression diminished in patients with NAFLD or cholestasis, but less known about the mechanisms its...
Interleukin 6 (IL-6), a key pleiotropic cytokine, plays vital role for hepatic function and regeneration. Augmenter of Liver Regeneration (ALR) is widely expressed co-mitogen with anti-apoptotic, anti-oxidative, anti-inflammatory properties, often dysregulated in liver diseases. Application ALR has been shown to dampen acute phase response (APR) triggered by IL-6. This study aimed unveil the underlying molecular mechanism how affects IL-6 signaling pathway using vitro experiments. Two...
Non-alcoholic fatty liver disease (NAFLD) encompasses a spectrum of hepatic pathologies, ranging from steatosis in its most benign form to cirrhosis advanced with non-alcoholic steatohepatitis (NASH) an intermediate level. NASH has been thought be triggered by various factors such as triglyceride accumulation hepatocytes and followed oxidant stress lipid peroxidation causing inflammation fibrosis. The progression Steatosis is facilitated when adaptive mechanisms protect acid-mediated...
Introduction: cholestasis is defined as the impairment of bile flow in liver leading to acids accumulation within liver, promoting inflammation and therefore causing injury. Previous studies indicated role up-regulation early growth response fator-1 (Egr-1) which initiates through up-regulating several pro-inflammatory genes. The hepatotrophic factor Augmenter regeneration (ALR), with anti-oxidative anti-apoptotic properties, was demonstrated support after hepatic Nevertheless, little known...
Nonalcoholic fatty liver disease (NAFLD) is a chronic hepatic caused by abnormal or excessive accumulation of fat in hepatocytes. NAFLD covers spectrum disorders ranging from simple steatosis (S) to nonalcoholic steatohepatitis (NASH) and cirrhosis, associated with changes gene expression profiles. Tissue profiling has been precious for developing diagnostic predictive biomarkers as well improving therapeutical strategies many diseases. Therefore, the aim this study was use microarray...