A5029037864- Pain Mechanisms and Treatments
- Neuropeptides and Animal Physiology
- Botulinum Toxin and Related Neurological Disorders
- Neuroscience of respiration and sleep
- Neuroscience and Neuropharmacology Research
- Olfactory and Sensory Function Studies
- Ion Channels and Receptors
- Nerve injury and regeneration
- Vestibular and auditory disorders
- Ion channel regulation and function
- Myofascial pain diagnosis and treatment
- Trigeminal Neuralgia and Treatments
- Temporomandibular Joint Disorders
- Biochemical effects in animals
- Laser Applications in Dentistry and Medicine
- Acupuncture Treatment Research Studies
- Dysphagia Assessment and Management
- Transcranial Magnetic Stimulation Studies
- Infrared Thermography in Medicine
- Biochemical Analysis and Sensing Techniques
- Neurobiology and Insect Physiology Research
- Musculoskeletal pain and rehabilitation
- Adenosine and Purinergic Signaling
- Salivary Gland Disorders and Functions
- Ocular Surface and Contact Lens
Nihon University
2015-2024
Surugadai Nihon University Hospital
2006-2024
University School
2022
Morpho (United States)
2011-2020
Osaka Prefectural Medical Center
2012-2013
Czech Academy of Sciences, Institute of Physiology
2009-2011
Hokuto (Japan)
2007
Fujita Health University Hospital
2004-2005
Japan Science and Technology Agency
2003
Osaka University
1998-2002
Alteration in the intracellular signal transduction pathway primary afferent neurons may contribute to pain hypersensitivity. We demonstrated that very rapid phosphorylation of extracellular signal-regulated protein kinases (pERK) occurred DRG were taking part transmission various noxious signals. The electrical stimulation Aδ fibers induced pERK primarily with myelinated fibers. c-Fiber activation by capsaicin injection small unmyelinated containing vanilloid receptor-1 (VR-1), suggesting...
The aim of this study was to investigate whether astroglia in the medullary dorsal horn (trigeminal spinal subnucleus caudalis; Vc) may be involved orofacial neuropathic pain following trigeminal nerve injury. effects intrathecal administration astroglial aconitase inhibitor sodium fluoroacetate (FA) were tested on Vc hyperactivity [as revealed by glial fibrillary acid protein (GFAP) labeling], nocifensive behavior, extracellular signal-regulated kinase phosphorylation (pERK), and neuronal...
The organization and response properties of nociceptive neurons in area 1 the primary somatosensory cortex (SI) anesthetized monkeys were examined. receptive fields classified as either wide-dynamic-range (WDR) that preferentially responsive to noxious mechanical stimulation, or specific (NS) only stimuli. cortical locations responses two classes compared. An examination neuronal stimulus-response functions obtained during thermal stimulation glabrous skin foot hand indicated WDR exhibited...
Studies at spinal levels indicate that peripheral tissue or nerve injury induces a state of hyperexcitability dorsal horn neurons participates in the development persistent pain and hyperalgesia. It has not been demonstrated orofacial region leads to similar central trigeminal system. The purpose present study was conduct parametric analysis response properties nociceptive nonnociceptive nucleus caudalis (medullary horn, MDH) rat model inflammation. Neurons were recorded extracellularly...
Background: It has been reported that the P2Y 12 receptor (P2Y R) is involved in satellite glial cells (SGCs) activation, indicating R expressed SGCs may play functional roles orofacial neuropathic pain mechanisms. However, involvement of mechanisms still unknown. We therefore studied reflex to noxious mechanical or heat stimulation tongue, and fibrillary acidic protein (GFAP) immunohistochemistries trigeminal ganglion (TG) a rat model unilateral lingual nerve crush (LNC) evaluate role SGC...
Abstract Oxytocin (OXT) is a neuropeptide hormone synthesized and secreted by hypothalamic neurons has been reported to play significant role in pain modulation. However, the mechanisms underlying OXT's antinociceptive effect on neuropathic are not fully understood. In this study, we examined peripheral of OXT mechanical hypersensitivity induced partial ligation infraorbital nerve (PNL) rats. Mechanical whisker pad skin after PNL was attenuated direct administration into trigeminal ganglion...
Accidental mandibular nerve injury may occur during tooth extraction or implant procedures, causing ectopic orofacial pain. The exact mechanisms underlying pain following is still unknown. Here, we investigated the role of macrophages and tumor necrosis factor alpha (TNFα) in trigeminal ganglion (TG) inferior alveolar transection (IANX). IANX was performed mechanical head-withdrawal threshold (MHWT) whisker pad skin ipsilateral to measured for 15 days. Expression Iba1 TG examined on day 3...
Clinically, it is well known that injury of mandibular nerve fiber induces persistent ectopic pain which can spread to a wide area the orofacial region innervated by uninjured trigeminal branches. However, exact mechanism such not still known. The present study was undertaken determine role connexin 43 in ganglion on mechanical hypersensitivity rat whisker pad skin induced inferior alveolar injury. Here, we examined changes sensitivity following Furthermore, expression and its localization...
Although peripheral nerves have an intrinsic self-repair capacity following damage, functional recovery is limited in patients. It a well-established fact that macrophages accumulate at the site of injury. Numerous studies indicate phenotypic shift from M1 macrophage to M2 plays crucial role process axon regeneration. This polarity change observed exclusively but not microglia and CNS macrophages. However, molecular basis axonal regeneration by yet fully understood. Herein, we aimed identify...
After transection of the inferior alveolar nerve (IAN), whisker pad area, which is innervated by infraorbital (ION) that was not injured, showed hypersensitivity to mechanical stimulation. Two days after IAN transection, threshold intensity for escape behavior stimulation ipsilateral area less than 4.0 g, indicating allodynia. A total 68 single fiber discharges were recorded from ION fibers at 3 transection. The responses C- and A-fibers classified according their conduction velocity....
Abstract An important function of the medullary dorsal horn (MDH) is relay nociceptive information from face and mouth to higher centers central nervous system. We studied projection pattern axons arising MDH by examining axonal transport Phaseolus vulgaris ‐leucoagglutinin (PHA‐L). Labeled axon terminal distributions were analyzed at light microscopic level. After large injections PHA‐L into both superficial deep laminae in rat, labeled observed nucleus submedius thalamus (SUB),...
Abstract In order to clarify the mechanisms underlying changes in primary afferent neurons trigeminal neuropathic pain, a chronic constriction nerve injury model of infraorbital (ION‐CCI) was developed rats. Mechanical allodynia observed at 3 days after ION‐CCI and lasted more than 14 days. Single‐unit activities were recorded from ION anesthetized C‐, Aβ‐ Aδ‐units identified on basis their conduction velocity. frequently encountered later period ION‐CCI. The highest Aδ‐spontaneous activity...
It has been reported that the anterior cingulate cortex (ACC) a variety of functions relating to pain as well perception. However, underlying mechanisms for those remain unclear. To elucidate functional role ACC in perception and pain-related such attention escape from pain, single neuronal activity was recorded ACC, behavioral correlates this studied. A total 667 neurons were awake behaving monkeys. Twenty-one had modulated during heat-detection task. Eighteen these increased their firing...
Abstract To define the somatotopic arrangement of neurons in trigeminal spinal subnucleus caudalis and upper cervical cord activated by acute noxious stimulation various orofacial sites, pERK expression was analyzed these neurons. After capsaicin injection into tongue, lower gum, lips, or mental region, pERK‐like immunoreactive (pERK‐LI) cells were distributed mainly dorsal half nucleus interporalis (Vi) (Vc) transition zone (Vi/Vc zone), middle Vc, Vc (Vc/C2 zone). pERK‐LI throughout to...
Background In addition to caudal subnucleus caudalis (Vc) of the spinal trigeminal complex, recent studies indicate that subnuclei interpolaris/caudalis (Vi/Vc) transition zone plays a unique role in processing deep orofacial nociceptive input. Studies also suggest glia and inflammatory cytokines contribute development persistent pain. By systematically comparing effects microinjection antiinflammatory cytokine interleukin (IL)-10 two glial inhibitors, fluorocitrate minocycline, we tested...
It is well known that oral inflammation causes tenderness in temporomandibular joints or masseter muscles. The exact mechanism of such an orofacial ectopic hyperalgesia remains unclear. Here, we investigated the functional significance interaction nerve growth factor (NGF) and transient receptor potential vanilloid 1 (TRPV1) relation to heat whisker pad skin caused by complete Freund's adjuvant (CFA) injection into lower lip. CFA induced ipsilateral skin. Moreover, it leads enhancement...