This study aimed at evaluating the antioxidative activities and safety of 50% ethanolic extract from red bean fermented by Bacillus subtillis IMR-NK1. The activities, including α,α-diphenyl-β-picryl-hydrazyl (DPPH) radicals scavenging effects, Fe2+-chelating ability, reducing power, were studied in vitro. It was found that activity increased with concentrations to a certain extent then leveled off as concentration further increased. As compared commercial antioxidants, showed less effect on...

Summary The objective of this study was to evaluate the effect fermentation time on antioxidant activity tempeh, a fermented product from soybean. Rhizopus oligosporus used ferment soybean for 0, 1, 2, 5 and 10 days. Lyophilised tempeh powder extracted with hexane followed by petroleum ether, 95% ethanol water. Antioxidant activities extracts were evaluated various models including α,α‐diphenyl‐β‐pricryl‐hydrazyl (DPPH) superoxide‐scavenging activities, reducing power inhibitory towards...

This study examines infant feeding beliefs and practices in a rural Peruvian Quechua community. The aim was to assess which foods are considered culturally meaningful, if concordance exists between practices. Mothers were asked free-list their child's "actual" first (n = 85), then "ideal" 87). Food lists analyzed for cultural salience. listed 43 foods; four (potato, mazamorra, egg, liver) salient on both lists. Some discordance found Culturally animal-source protein-iron rich, whereas...

Lignocellulosic biomass is an attractive low-cost feedstock for bioethanol production. During production, Saccharomyces cerevisiae, the common used starter, faces several environmental stresses such as aldehydes, glucose, ethanol, high temperature, acid, alkaline and osmotic pressure. The aim of this study was to construct a genetic recombinant S. cerevisiae starter with tolerance against various stresses. Trehalose-6-phosphate synthase gene (tps1) aldehyde reductase (ari1) were...

A genetic recombinant Saccharomyces cerevisiae starter with high ethanol tolerance capacities was constructed. In this study, the gene of trehalose-6-phosphate synthase (encoded by tps1), which catalyzes first step in trehalose synthesis, cloned and overexpressed S. cerevisiae. Moreover, neutral trehalase nth1, degrading enzyme) deleted using a disruption cassette, contained long flanking homology regions nth1 (the upstream 0.26 kb downstream 0.4 kb). The engineered strain increased its...

Summary The effects of blanching, drying and extraction processes on the antioxidant activities one kind Taiwanese yam peel, Darsan ( Dioscorea alata ), were investigated. measurements included total phenolic content, reducing power α ,α‐diphenyl‐β‐pricryl‐hydrazyl (DPPH) radical‐scavenging activity. 50% ethanolic, hot water extracts from peel all had much higher than those flesh. Among three methods, ethanolic resulted in highest while was more appropriate for Blanching by immersing 85 °C...

ABSTRACT The effects of high hydrostatic pressure (HHP) on gel strength Pacific whiting and Alaska pollock surimi were determined by torsion. gels made with without 1% beef plasma protein (BPP) as protease inhibitor. HHP treated (1% BPP added) showed greatly increased strain values at all pressure/temperature combinations compared heat‐set controls. Stress for the same samples variable depending treatment species. A three‐fold increase in stress was found

Two thermally stable chitosanase isoforms were purified from the sheaths of chitosan-treated bamboo shoots. Isoforms A and B had molecular masses 24.5 16.4 kDa isoelectric points 4.30 9.22, respectively. Using chitosan as substrate, both functioned optimally between pH 3 4, optimum temperatures for activities 70 60 °C, The kinetic parameters K(m) V(max) isoform 0.539 mg/mL 0.262 μmol/min/mg, respectively, 0.183 0.092 Chitosans susceptible to degradation by enzymes could be converted low...

Red bean (Phaseolus radiatus L. var. Aurea) is a leguminous seed and mainly used as one of the popular ingredients in oriental desserts. The objective this study was to evaluate anti-inflammatory activity 50 g/kg ethanolic extract red (RBE) by measuring lipopolysaccharide (LPS)-induced expressions nitric oxide (NO), inducible synthase (iNOS), cyclooxygenase 2 (COX-2), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) RAW 264.7 macrophages. On other hand, antioxidant RBE determined...

Using 4-methylumbelliferyl-β-D-N,N',N″-triacetylchitotrioside (4-MU-GlcNAc3) as a substrate, an acidic chitinase was purified from seeds of black soybean (Glycine max Tainan no. 3) by ammonium sulfate fractionation and three successive steps column chromatography. The monomeric enzyme with molecular mass 20.1 kDa isoelectric point 4.34. catalyzed the hydrolysis synthetic substrates p-nitrophenyl N-acetyl chitooligosaccharides chain length 3 to 5 (GlcNAcn, n = 3-5), pNp-GlcNAc4 most...

Human α-defensin 5 (HD5), a small cationic peptide, is expressed in Paneth cell granules of intestinal crypts. HD5 exhibits high antimicrobial activity against broad spectrum pathogenic agents, including bacteria, fungi, and viruses. In this study, the constitutive expression peptide was achieved using methylotrophic yeast, Pichia pastoris (P. pastoris). cDNA amplified by polymerase chain reaction (PCR) human lung as template. The 96-bp DNA fragment encoding mature (amino acid 63-94)...

A chitosanase was purified from jelly fig latex by ammonium sulfate fractionation (50–80% saturation) and three successive column chromatography steps. The enzyme almost homogeneous, as determined SDS-polyacrylamide gel electrophoresis (SDS-PAGE) activity staining. molecular mass of the 20.5 kDa. isoelectric point (pI) <3.5, estimated focusing on PhastGel IEF 3-9. Using chitosan substrate, optimal pH for reaction 4.5; kinetic parameters Km Vmax were 0.089 mg mL-1 0.69 μmol min-1 mg-1,...