Lately, major advances in crucial aspects of magnetic hyperthermia (MH) therapy have been made (nanoparticle synthesis, biosafety, etc.). However, there is one key point still lacking improvement: the field-frequency product (H × f = 4.85 108 Am-1s-1) proposed by Atkinson-Brezovich as a limit for MH therapies. Herein, we analyze both local and systemic physiological effects overpassing this limit.Different combinations field frequency intensity exceeding (591-920 kHz, 10.3-18 kA/m) applied...

This work reports important advances in the study of magnetic nanoparticles (MNPs) related to their application different research fields such as hyperthermia. Nanotherapy based on targeted could become an attractive alternative conventional oncologic treatments it allows a local heating tumoral surroundings without damage healthy tissue. RGD-peptide-conjugated MNPs have been designed specifically target α V β 3 receptor-expressing cancer cells, being bound RGD peptides by “click chemistry”...

Abstract Introduction The deprivation of blood supply to an intestinal segment, followed by its subsequent restoration, triggers the condition known as ischaemia-reperfusion syndrome (i-IRI). Throughout this process, proinflammatory and oxidative mediators are produced, released, distributed via bloodstream, ultimately causing damage both locally systemically. Cannabidiol (CBD) is a molecule with antioxidant anti-inflammatory properties that regulate stress inflammatory states. aim study...

The need for new and effective treatments neonates suffering from hypoxia–ischemia is urgent, as the only implemented therapy in clinics therapeutic hypothermia, 50% of cases. Cannabinoids may modulate neuronal development brain plasticity, but further investigation needed to better describe their implication a neurorestorative after neonatal HI. cannabinoid URB447, CB1 antagonist/CB2 agonist, has previously been shown reduce injury HI, it not clear whether sex affect its neuroprotective...

Background: New therapeutic approaches are an essential need for patients suffering from colorectal cancer liver metastases. Curcumin, a well-known plant-derived polyphenol, has been shown to play role in the modulation of multiple signaling pathways involved development and progression certain cells vitro. This study aims assess anti-tumor effect curcumin on CC531 cells, both vitro vivo. Methods: On cultures, cell viability migration capacity were analyzed (wound healing test) 24, 48, 72 h...

Intestinal ischemia-reperfusion injury (i-IRI) involves a blood flow interruption in an intestinal segment followed by restoration. When is restored, oxidative and inflammatory molecules are distributed throughout the bloodstream, triggering both local systemic damage. Our goal was to evaluate potential of three antioxidant and/or anti-inflammatory compounds (curcumin, dexmedetomidine α-tocopherol) prevent or reverse damage induced i-IRI.i-IRI placing microvascular clip superior mesenteric...

Intestinal ischemia-reperfusion injury (i-IRI) is a rare disorder with high mortality rate, resulting from the loss of blood flow to an intestinal segment. Most damage triggered by restoration and arrival cytokines reactive oxygen species (ROS), among others. Inactivation these molecules before tissue reperfusion could reduce damage. The aim this work was analyze preventive effect allopurinol nitroindazole on mucosal after i-IRI. Wag/RijHsd rats were subjected i-IRI clamping superior...

Abstract Current methodology described to mimic lower limb ischaemia–reperfusion injury (LL-IRI) does not accurately define the procedures and pressures exerted induce maintain ischaemia. In this piece of work, we propose a well-defined detailed rat model that simulates conditions established in clinical practice guidelines for tourniquet application allows us test treatments aim prevent/reduce LL-IRI. Eighty-six male WAG/RijHsd rats were subjected hind IRI (LL-IRI), using mechanical system...

Surgery under ischemic conditions, lasting up to 3 h, is routinely performed in orthopedic surgery, causing undesirable injury due ischemia-reperfusion syndrome, with short and medium-term functional repercussions. To date, there no established prophylactic treatment. In this work we evaluated folinic acid (FA) a rodent model of lower limb (IRI-LL). 36 male WAG rats underwent h ischemia. the saline group, received intraperitoneal administration (used as vehicle for treatment). experimental...

Retinal ischemia-reperfusion (IR) injury occurs in pathological situations that interrupt the blood flow to retina, such as is case during central retinal artery occlusion (CRAO). The animal models described literature are based on pressure produced by weight of a given quantity saline elevated certain height; however, establish these parameters it necessary perform mathematical calculations cannot be easily redone punctual variations intraocular (IOP). aim this study was present new system...

Background: Lower limb ischemia-reperfusion injury (IRI-LL) is a common major complication of orthopedic surgery, especially in elderly patients. It has previously been demonstrated that folinic acid (FA) reduced IRI-LL damage 3−4-month-old rats. This current work analyses the effect FA prevention animals. Methods: Forty-two 18-month-old male WAG/RijHsd rats were subjected to 3 h ischemia. Eighteen animals received (2.5 mg/kg, ip) 20 min before end ischemia period, while other half same...

Human skin exposure to ultraviolet B (UVB) radiation can result in acute photodamage through oxidative modifications of cellular components and biomolecules involved the metabolism dermal cells. Recently, therapeutic potential human adipose-derived stem cells (hASCs) has been investigated as a novel strategy for photoprotection due their pro-angiogenic properties, protective activity against stress paracrine effect on To enhance these hASCs be preconditioned by exposing them sublethal...

<b><i>Background:</i></b> Due to their self-renewal, proliferation, differentiation, and angiogenesis-inducing capacity, human adipose mesenchymal stem cells (AMSC) have potential clinical applications in the treatment of limb ischemia. AMSC from healthy donors been shown induce neovascularization animal models. However, when were obtained suffering any pathology, autologous application showed limited effectiveness. We studied whether liposuction niche obesity could...

Patient safety (PS) is a key factor in reducing or even eradicating adverse incidents and events. Many health organizations promote strategies to improve PS, while also pointing out the importance of measuring it. For more than eight years, our institution has developed focused on improving PS-culture among personnel. The goal this paper analyze between years 2009 2017.A cross-sectional survey by American Agency for Healthcare Research Quality (AHRQ), was conducted 2017 all healthcare...

Background: Hyperthermia (HT) therapy still remains relatively unknown, in terms of both its biological and therapeutic effects. This work aims to analyze the effects exposure HT, such as that required anti-tumor magnetic hyperthermia therapies, using metabolomic serum parameters routinely analyzed clinical practice. Methods: WAG/RigHsd rats were assigned different experimental groups needed emulate all procedures involved treatment liver metastases by HT. Twelve hours or ten days after...

Abstract Introduction The use of tourniquet to achieve a blood-free surgical field is common practice in orthopedic surgery. Blood flow restoration may lead systemic inflammatory and oxidative stress damage known as ischemia/reperfusion injury (IRI). aim this work evaluate whether the administration dexketoprofen (DEX) before reperfusion contributes prevention IRI-related damage. Methods Male WAG/RijHsd rats were divided into 3 groups: two them underwent 3h ischemia administered either (1...

Abstract Introduction The preferred treatment for patients bearing liver metastases (CRCLM) quite often includes biologic therapies such as cetuximab (anti-EGFR). aim of this piece work was to evaluate the effect a cytoreductive agent CRCLM in murine model selective portal ligation (PVL). Methods Male WAG/RijHsd rats were used study; thirty MHCCR-bearing animals subjected PVL and pretreated with (12) or vehicle (12), another six did not receive any treatment. In addition, three groups...

Neonatal hypoxia–ischemia is a major cause of infant death and disability. The only clinically accepted treatment therapeutic hypothermia; however, cooling less effective in the most severely encephalopathic infants. Here, we wanted to test neuroprotective effect antioxidant dimethyl fumarate after severe neonatal rats. We used modified Rice–Vannucci model generate hypoxic–ischemic brain damage day 7 postnatal rats, which were randomized into four experimental groups: Sham, Sham + DMF,...

Abstract Partial hepatectomy (PHx) is the gold standard for treatment of colorectal cancer liver metastases. However, after removing a substantial amount hepatic tissue, growth factors are released to induce regeneration, which may promote proliferation micrometastases or circulating tumour cells still present in patient. The aim this study assess effect PHx on metastases induced by intrasplenic cell inoculation as well vitro same line. Liver tumours were 18 WAG/RijHsd male rats, seeding...

Nowadays, surgical excision remains the gold standard to treat liver metastases of colorectal cancer (CRCLM). However, as more than 50% patients are not eligible for surgery, other alternatives such percutaneous or intravascular interventional therapies (thermal ablation, chemoembolization, radioembolization), quite relevant. Recently, use magnetic nanoparticles (MNPs) has been suggested an adjuvant these therapies, they could increase their necrotising effect on tumour while reducing doses...

Liver resection remains the gold standard for hepatic metastases. The future liver remnant (FLR) and its functional status are two key points to consider before performing major resections, since patients with less than 25% FLR or a Child-Pugh B C grade not eligible this procedure. Folinic acid (FA) is an essential agent in cell replication processes. Herein, we analyze effect of FA as enhancer regeneration after selective portal vein ligation (PVL). Sixty-four male WAG/RijHsd rats were...