In vivo studies have shown that amnion-produced growth factors participate in many diseases involve angiogenesis, re-epithelialization and immunomodulation. Although human amniotic epithelial cells (hAECs) mesenchymal stem (hAMSCs) can be obtained from membranes, there is little information regarding their biological differences. The aim of the present study was to isolate characterize amnions, investigate potential behavior these on function endothelial vitro examine variations expression...

Although transplantation of human mesenchymal stem cells (MSCs) derived from amnion (hAMSCs), bone marrow (hBMSCs) and adipose tissues (hADSCs) has been shown to aid in the repair cutaneous wounds mouse models, little information is available regarding relative efficacy MSCs different sources. In this study, we compared their therapeutic potentials by transplanting equal numbers hAMSCs, hBMSCs or hADSCs a model wounds. The results suggested that an hADSC injection most pronounced effect on...

Abstract Neural stem cell (NSC) proliferation and differentiation play a pivotal role in the repair of brain function central nervous system (CNS) diseases. Radial extracorporeal shock wave therapy (rESWT) is non-invasive innovative treatment for many conditions, yet little known about effects this on NSCs. Mouse NSCs (NE-4C) were exposed to rESWT with 1.0, 1.5, 2.0, 2.5, 3.0, 3.5 bar (500 impulses, 2 Hz) vitro . Cell viability test results indicated that rESWT, at dose 2.5 bar, 500 Hz,...

Glioblastoma (GBM) is the most common primary brain tumor, with poor prognosis and a lack of effective therapeutic options. The aberrant expression transcription factor REST (repressor element 1-silencing factor) had been reported in different kinds tumors. However, function its mechanisms GBM remain elusive. Here, was inhibited by siRNA silencing U-87 U-251 cells. Then CCK-8 assay showed significantly decreased cell proliferation, inhibition migration verified scratch wound healing...

MicroRNA (miRNA) is a kind of short non-coding RNA, involved in various cellular processes. During keratinocyte differentiation, miRNAs act as important regulators. In this study, we demonstrated by microarray assay that the expression miR-378b significantly increased during keratinocytes differentiation. Our findings showed could inhibit proliferation, migration and differentiation keratinocytes. Luciferase reporter assays directly target NKX3.1. Silencing NKX3.1 coincide with effects...

A fibrotic or pathological scar is an undesired consequence of skin wound healing and may trigger a series problems. An attenuated inflammatory response significant characteristic fetal healing, which can contribute to the scarless skin. According deep sequencing data, microRNA‑149 (miR‑149) expression was increased in mid-gestational compared with that late‑gestational keratinocytes. It demonstrated overexpression miR‑149 HaCaT cells downregulate pro‑inflammatory cytokines interleukin...

Both apoptosis and caspase-3 activity in adipose tissue-derived stem cells play an important role the therapeutic process of diabetes patients. The purpose this study was to investigate effect advanced glycation end products-human serum albumin (AGE-HSA) on human (ADSCs) characterize signal transduction pathways activated by AGEs that are involved regulation.AGE-HSA promoted ADSCs. However, effects AGE-HSA were significantly attenuated inhibitor p38 MAPK, but not inhibitors JNK MAPK or ERK...

Titanium (Ti) and Ti-based alloys are widely used in the manufacture of dental orthopedic implants. However, how to improve their osteogenic differentiation ability is still a key issue be resolved. In this study, gradient nanostructured surface (GNS) samples were prepared by mechanical grinding treatment, coarse-grained (CG) obtained recrystallization annealing, making sure that two kinds specimens had similar roughness. Then, human amniotic mesenchymal stem cells (hAMSCs) cocultured with...

Keratinocytes proliferation is critical for the capacity to heal wounds and accumulating evidences have proved that dysregulation of microRNAs involved in keratinocytes. However, molecular mechanisms remain be completely elucidated. Here, we show miR-136 was significantly decreased by TGF- β 1 treatment HaCaT cells normal human epidermal keratinocytes (NHEK), it a Smad3-dependent manner. By cell assay cycle analysis, found reintroduction transfection, as well PPP2R2A silencing, counteracted...

Stem cells derived from human amniotic membrane (hAM) are promising targets in regenerative medicine. A previous study focused on stem skin wound and scar‑free healing. The present aimed to investigate whether hydrogen peroxide (H2O2)‑induced senescence of dermal fibroblasts (hDFs) was influenced by the anti‑aging effect conditioned medium (CdM) cells. First, biological function two types cells, namely epithelial (hAECs) mesenchymal (hAMSCs), hDFs compared. results cell proliferation healing...

Mesenchymal stem cells (MSCs) show immunosuppressive activities and alleviate atherosclerosis (AS) formation in apolipoprotein E‑knockout (apoE‑KO) mice. Human amnion mesenchymal (hAMSCs), a particular population of cells, have been shown to immunomodulatory abilities. The present study investigated the effects hAMSCs treatment on early atherosclerotic plaque progression established lesion apoE‑KO In total, 36 mice were fed with high‑fat diet. Mice subjected hAMSCs‑injection simultaneously...

Background Early- to mid-gestational fetal mammalian skin wounds heal rapidly and without scarring. Keratinocytes (KCs) have been found exert important effects on the regulation of fibroblasts. There may be significant differences gestational KCs at different ages. The advantages in early- could lead scarless wound healing. Methods from six human samples were divided into two groups: a mid-gestation group (less than 28 weeks age) late-gestation (more age). RNA extracted was used prepare...

The repair strategy for the healing of skin wounds in fetuses differs from that adults. Proliferation and migration dermal fibroblasts are main mechanisms associated with wound healing, as well complex interactions between epidermal keratinocytes (KCs) fibroblasts. In order to investigate effects fetal KCs on adult human fibroblasts, were isolated tissue mid‑gestational adults, co‑cultured using a Transwell® system. When either or proliferative migratory potential was significantly enhanced....

Mesenchymal stem cells (MSCs) are emerging as a promising therapeutic approach and genetic modification by non-viral vectors could span their applications. Herein, the capacity of PEI-coated Fe3O4 nanoparticles (NPs) for siRNA delivery to human MSCs derived from different tissues was evaluated. The prepared spherical NPs with charge around +44.0 mV an average diameter 12 nm exhibited proper encapsulation enhanced serum stability. High-purity were isolated bone marrow, adipose tissue amnion...

Early in gestation, wounds fetal skin heal by regeneration, which microRNAs play key roles. Seq-915_x4024 is a novel microRNA candidate confirmed deep sequencing and mirTools 2.0. It highly expressed keratinocytes during early gestation. Using an vitro wound-healing assay, Transwell cell migration MTS proliferation we demonstrated that overexpressing seq-915_x4024 exhibited higher proliferative activity the ability to promote fibroblast proliferation. These characteristics of are same...

Since the beginning of use stem cells in tissue regenerative medicine, there has been a search for optimal sources cells. Human amniotic epithelial (hAECs) are derived from human amnions, which typically discarded as medical waste, but were recently found to include with trilineage differentiation potential vitro. Previous study focused on osteogenic ability hAECs seed bone regeneration; however, their paracrine effects osteoblasts (OBs) yet be elucidated. In present study, conditioned...

In this study, we identified wound healing-related proteins secreted by human amniotic epithelial cells (hAECs) and mesenchymal stem (hAMSCs). We observed increased migration reduced proliferation differentiation when keratinocytes were co-cultured in media conditioned hAECs (hAECs-CM) hAMSCs (hAMSCs-CM). Label-free mass spectrometry bioinformatic analyses of the hAECs-CM hAMSCs-CM proteome revealed several associated with healing, angiogenesis, cellular differentiation, immune response cell...

Well-controlled trophoblast invasion at the maternal-fetal interface is crucial for normal placentation and successful pregnancy, otherwise pathological conditions of pregnancy occur, such as preeclampsia. In previous studies, it has been demonstrated that unique long 16 binding protein (ULBP)1, a ligand natural-killer group (NKG)2D receptor on uterine natural killer (uNK) cells, upregulated in placenta patients with As they are present majority decidua, uNK have an important role pregnancy....

Mesenchymal stem cells (MSCs) have been a potential strategy in the pretreatment of pulmonary diseases, while mechanisms MSCs-conditioned medium (MSCs-CM) involved with microRNAs on regulation lung ion transport are seldom reported. We investigated role miR-124-5p lipopolysaccharide-involved epithelial sodium channel (ENaC) dysfunction and explored target miR-124-5p. observed lower expression after administration MSCs-CM, overexpression or inhibition regulated α -subunit ( -ENaC) at protein...