A5038449839- Olfactory and Sensory Function Studies
- Neural dynamics and brain function
- Advanced MRI Techniques and Applications
- Functional Brain Connectivity Studies
- Advanced Chemical Sensor Technologies
- Photoreceptor and optogenetics research
- Neuroscience and Neuropharmacology Research
- Photoacoustic and Ultrasonic Imaging
- Neurobiology and Insect Physiology Research
- Biochemical Analysis and Sensing Techniques
- Optical Imaging and Spectroscopy Techniques
- Stroke Rehabilitation and Recovery
- Atomic and Subatomic Physics Research
- Sparse and Compressive Sensing Techniques
- Transcranial Magnetic Stimulation Studies
- Neurological Disease Mechanisms and Treatments
- NMR spectroscopy and applications
- Botulinum Toxin and Related Neurological Disorders
- Cell Image Analysis Techniques
- Advanced Fluorescence Microscopy Techniques
- Advanced Neuroimaging Techniques and Applications
University of Pittsburgh
2014-2024
McGowan Institute for Regenerative Medicine
2023-2024
Emory University
2011
Introducing optogenetics into neurovascular research can provide novel insights the cell-specific control of hemodynamic response. To generalize findings from molecular approaches, it is crucial to determine whether light-activated circuits have same effect on vasculature as sensory-activated ones. For that purpose, rats expressing channelrhodopsin (ChR2) specific excitatory glutamatergic neurons were used measure neural activity, blood flow, hemoglobin-based optical intrinsic signal, and...
High-resolution functional magnetic resonance imaging (fMRI) detects localized neuronal activity via the hemodynamic response, but it is unclear whether accurately identifies specific to individual layers. To address this issue, we preferentially evoked in superficial, middle, and deep layers of rat olfactory bulb: glomerular layer by odor (5% amyl acetate), external plexiform electrical stimulation lateral tract (LOT), granule cell anterior commissure (AC), respectively. Electrophysiology,...
Functional MRI responses are localized to the synaptic sites of evoked inhibitory neurons, but it is unknown whether, or by what mechanisms, these neurons initiate functional hyperemia. Here, neuronal origins hemodynamic were investigated fMRI local field potential and blood flow measurements during topical application pharmacological agents when GABAergic granule cells in rat olfactory bulb synaptically targeted. First, examine if postsynaptic activation was required for neurovascular...
Intracerebral cell therapy (CT) is emerging as a new therapeutic paradigm for stroke. However, the impact of physical (PT) on implanted cells and their ability to promote recovery remains poorly understood. To address this translational issue, clinical-grade neural stem (NSC) line was into peri-infarct tissue using MRI-defined injection sites, two weeks after PT in form aerobic exercise (AE) administered 5 × per week post-implantation commonly applied patients with A combined AE CT exerted...
Functional MRI (fMRI) measures neural activity indirectly by detecting the signal change associated with hemodynamic response following brain activation. In order to alleviate temporal and spatial specificity problems fMRI, a number of attempts have been made detect magnetic fields (NMFs) directly, but thus far provided conflicting results. this study, we used MR axonal NMFs in median giant fiber earthworm, Lumbricus terrestris, examining free induction decay (FID) sampling interval 0.32 ms....
Currently, clinical assessment of olfaction is largely reliant on subjective methods that require patient participation. The objective method for measuring olfaction, using electroencephalogram (EEG) readings, can be supplemented with the improved temporal resolution magnetoencephalography (MEG) olfactory measurement delineate cortical and peripheral loss. MEG provides high spatial which enhance our understanding central processing compared to EEG alone.To determine feasibility building an...
Abstract Awake rodent fMRI is increasingly becoming a reliable neuroimaging technique to study neuronal activity at both the whole brain and high-resolution laminar scales. Prior studies have focused on developing acclimation protocols, experimental paradigms, hardware optimize outcomes. However, little effort has been made address impact of pulse sequence selection detecting activation in awake experiments. In current study, we compare gradient-echo echo planar imaging (GE-EPI)...
Abstract Background Preclinical Alzheimer’s disease research has gained traction as a potential point of intervention, though it is relatively unknown how early stages the impact cortical health. The following study utilizes optical imaging methods (Figure 1) to characterize changes in neuronal, glutamate, and hemodynamic activities preclinical amyloidosis mouse model disease. Method Five (n = 5; 2 females & 3 males) APPswe/PS1dE9 x Thy1‐jRGECO1a double transgenic mice were breed for...
Awake rodent fMRI is increasingly common over the use of anesthesia since it permits behavioral paradigms and does not confound normal brain function or neurovascular coupling. It well established that adequate acclimation to loud environment head fixation reduces stress in rodents allows for whole imaging with little contamination from motion. However, unknown whether high-resolution increased susceptibility motion lower sensitivity can measure small, but spatially discrete, activations...