A5004551013- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Influenza Virus Research Studies
- Monoclonal and Polyclonal Antibodies Research
- Viral Infections and Vectors
- Respiratory viral infections research
- Viral gastroenteritis research and epidemiology
- Gut microbiota and health
- Cytomegalovirus and herpesvirus research
- IL-33, ST2, and ILC Pathways
- Immunodeficiency and Autoimmune Disorders
- Atherosclerosis and Cardiovascular Diseases
- Viral-associated cancers and disorders
- Salmonella and Campylobacter epidemiology
- Virology and Viral Diseases
- Inflammatory Bowel Disease
- Lymphoma Diagnosis and Treatment
- Viral Infections and Outbreaks Research
- Single-cell and spatial transcriptomics
- Cancer-related molecular mechanisms research
- Chemical Reactions and Isotopes
- Herpesvirus Infections and Treatments
- Extracellular vesicles in disease
- dental development and anomalies
- Regional Socio-Economic Development Trends
Ministry of Foreign Affairs
2023
Wellcome Sanger Institute
2013-2020
Scripps Research Institute
2018
University of Oxford
2013
University College London
2013
University of Hong Kong
2013
University of Edinburgh
2013
A novel betacoronavirus associated with lethal respiratory and renal complications was recently identified in patients from several countries the Middle East. We report deep genome sequencing of virus directly a patient's sputum sample. Our high-throughput yielded substantial depth sequence assembly showed minority viral variants specimen. Detailed phylogenetic analysis (England/Qatar/2012) revealed its close relationship to European bat coronaviruses circulating among species...
Measles causes immunosuppression by restricting naïve B cell diversity and eliminating previously established memory cells.
Abstract Profiling immunoglobulin (Ig) receptor repertoires with specialized assays can be cost-ineffective and time-consuming. Here we report ImReP, a computational method for rapid accurate profiling of the Ig repertoire, including complementary-determining region 3 (CDR3), using regular RNA sequencing data such as those from 8,555 samples across 53 tissues types 544 individuals in Genotype-Tissue Expression (GTEx v6) project. Using ImReP GTEx v6 data, generate collection 3.6 million...
ABSTRACT Norovirus is a highly transmissible infectious agent that causes epidemic gastroenteritis in susceptible children and adults. infections can be severe initiated from an exceptionally small number of viral particles. Detailed genome sequence data are useful for tracking norovirus transmission evolution. To address this need, we have developed whole-genome deep-sequencing method generates entire sequences amounts clinical specimens. This novel approach employs algorithm reverse...
Seasonal influenza viruses create a persistent global disease burden by evolving to escape immunity induced prior infections and vaccinations. New antigenic variants have substantial selective advantage at the population level, but these are rarely selected within-host, even in previously immune individuals. Using mathematical model, we show that temporal asynchrony between within-host virus exponential growth antibody-mediated selection could limit evolution. If for new acts principally...
A diverse B-cell receptor (BCR) repertoire is required to bind a wide range of antigens. BCRs are generated through genetic recombination and can be diversified somatic hypermutation (SHM) or class-switch (CSR). Patterns diversity vary substantially between different health conditions. We use isotype-resolved BCR sequencing compare evolution fate in healthy individuals patients with chronic lymphocytic leukaemia (CLL). show that the patterns SHM CSR B-cells from distinct CLL. identify...
Lymphoblastoid cell lines (LCLs) are commonly used in molecular genetics, supplying DNA for the HapMap and 1000 Genomes Projects, to test chemotherapeutic agents, informing basis of a number population genetics studies gene expression. The process transforming human B cells into LCLs requires presence Epstein-Barr virus (EBV), double-stranded which through B-cell immortalisation maintains an episomal genome every LCL at variable copy numbers. Previous have reported that EBV alters host-gene...
Summary Crohn’s disease (CD) is a complex inflammatory disorder of incompletely understood molecular aetiology. We generated large single-cell RNA sequencing dataset from the terminal ileal biopsies two independent cohorts comprising total 50 CD patients and 71 healthy controls. performed transcriptomic analyses to reveal genes, cell types mechanisms perturbed in CD, leveraging power confirm our findings assess replicability. In addition mapping widespread alterations cytokine signalling, we...
<ns4:p><ns4:italic><ns4:bold>Background</ns4:bold>:</ns4:italic>Human memory B cells play a vital role in the long-term protection of host from pathogenic re-challenge. In recent years importance number different cell subsets that can be formed response to vaccination or infection has started become clear. To study responses, cultured<ns4:italic>ex vivo,</ns4:italic>allowing for an increase and activation these quiescent cells, providing sufficient quantities each subset enable full...
Human memory B cells play a vital role in the long-term protection of host from pathogenic re-challenge. In recent years importance number different cell subsets that can be formed response to vaccination or infection has started become clear. To study responses, cultured
Abstract Seasonal influenza viruses create a persistent global disease burden by evolving to escape immunity induced prior infections and vaccinations. New antigenic variants have substantial selective advantage at the population level, but these are rarely selected within-host, even in previously immune individuals. Using mathematical model, we show that temporal asynchrony between within-host virus exponential growth antibody-mediated selection could limit evolution. If for new acts...
Abstract Gastrointestinal microbiota and immune cells interact closely display regional specificity, but little is known about how these communities differ with location. Here, we simultaneously assess single across the healthy, adult human colon, paired characterisation of in mesenteric lymph nodes, to delineate colonic niches at steady-state. We describe distinct T helper cell activation migration profiles along colon characterise transcriptional adaptation trajectory regulatory between...
Seasonal influenza viruses create a persistent global disease burden by evolving to escape immunity induced prior infections and vaccinations. New antigenic variants have substantial selective advantage at the population level, but these are rarely selected within-host, even in previously immune individuals. We find that temporal asynchrony between within-host virus exponential growth antibody-mediated selection can limit evolution. If for new acts principally point of initial inoculation,...
Acute intestinal infections remain a significant problem for the majority of economically developed countries. infection disease incidence in the structure infectious pathology steadily ranks 2nd place after acute virus respiratory infections. The urgency of the problem is connected with the frequent occurrence clusters cases ( outbreaks ) .The last mass and largest in last 20 years outbreak acute intestinal , was detected St. Petersburg 2013. The Russian Federation takes one first places...
The paper presents the results of medical genetic examination 10 populations Karachay-Cherkess Republic (KCR) describing epidemiology (including molecular epidemiology) hereditary diseases. Molecular confirmation diagnosis was performed for 181 patients by PCR, MLPA and Sanger sequencing. three most common inherited autosomal recessive diseases (cystic fibrosis, sensorineural hearing loss phenylketonuria) studied in main ethnic groups KCR (Karachays, Russians, Cherkess, Abazins, Nogais)....