A5040670585- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Systemic Lupus Erythematosus Research
- Monoclonal and Polyclonal Antibodies Research
- HIV Research and Treatment
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Immunotherapy and Immune Responses
- Systemic Sclerosis and Related Diseases
- Cancer Immunotherapy and Biomarkers
- COVID-19 Impact on Reproduction
- Glycosylation and Glycoproteins Research
- NF-κB Signaling Pathways
- Syphilis Diagnosis and Treatment
- Optical Coherence Tomography Applications
- Radiomics and Machine Learning in Medical Imaging
- Bacterial Infections and Vaccines
- Immune Response and Inflammation
- Cytomegalovirus and herpesvirus research
- Dermatologic Treatments and Research
- Medical Imaging Techniques and Applications
- Complement system in diseases
- Reproductive tract infections research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Renal Diseases and Glomerulopathies
University College London
2022-2024
Imperial College London
2017-2023
University of Manchester
2022
Centre for Immunity, Infection and Evolution
2022
King's College London
2015-2018
National Institute of Allergy and Infectious Diseases
2018
International AIDS Vaccine Initiative
2018
Guy's Hospital
2017
The molecular heterogeneity of autoimmune and inflammatory diseases has been one the main obstacles to development safe specific therapeutic options. Here, we evaluated diagnostic clinical value a robust, inexpensive, immunoassay detecting circulating soluble form monocyte-specific surface receptor sialic acid binding Ig-like lectin 1 (sSIGLEC-1). We developed an measure sSIGLEC-1 in small volumes plasma/serum from systemic lupus erythematosus (SLE) patients (n = 75) healthy donors 504)....
IFN-γ-producing T helper 1 (Th1) cell responses mediate protection against infections but uncontrolled Th1 activity also contributes to a broad range of autoimmune diseases. Autocrine complement activation has recently emerged as key in the induction and contraction human immunity: regulator CD46 C3aR expressed by CD4+ cells via autocrine generated ligands C3b C3a, respectively, are critical IFN-γ production. Further, CD46-mediated signals induce co-expression immunosuppressive IL-10...
We assessed a cohort of people living with human immunodeficiency virus (PLWH) (n = 110) and HIV negative controls 64) after 1, 2 or 3 SARS-CoV-2 vaccine doses. At all timepoints, PLWH had significantly lower neutralizing antibody (nAb) titers than HIV-negative controls. also observed delayed development neutralization in that was underpinned by reduced frequency spike-specific memory B cells (MBCs). Improved breadth seen against the Omicron variant (BA.1) third dose but nAb responses...
Toll like receptor (TLR) ligands are important adjuvant candidates, causing antigen presenting cells to release inflammatory mediators, leading the recruitment and activation of other leukocytes. The aim this study was define response human blood derived dendritic macrophages three TLR acting singly or in combination, Poly I:C (TLR3), GLA (TLR4) R848 (TLR7/8). Combinations agonists have been shown a synergistic effect on individual cytokines, here we look at global measuring both cytokines...
Abstract We report the development of a novel flow cytometry–based Ig capture assay (ICA) for identification and sorting individual Ab-secreting cells based on their Ag reactivity. The ICA represents fast versatile tool single-cell peripheral plasmablasts, streamlining subsequent Ab analysis, cloning. demonstrate utility by isolating Ag-reactive plasmablasts from cryopreserved PBMC obtained volunteers vaccinated with recombinant HIV envelope protein. To show specificity ICA, we produced...
Polarisation-sensitive optical coherence tomography (PS-OCT) offers a novel, non-invasive method of assessing skin fibrosis in the multisystem disease systemic sclerosis (SSc) by measuring collagen retardance. This study aimed to assess retardance as biomarker SSc. Thirty-one patients with SSc and 27 healthy controls (HC) underwent PS-OCT imaging. 'Skin score' was assessed clinical palpation (0-3 scale). A subset ten age/sex-matched HC had biopsy longitudinal Histological assessment included...
Summary Patients with haematological malignancies are more likely to have poor responses vaccination. Here we provide detailed analysis of the humoral and cellular COVID‐19 vaccination in 69 patients B‐cell malignancies. Measurement anti‐spike IgG serum demonstrated a low seroconversion rate 27.1% 46.8% seroconverting after first second doses vaccine, respectively. In vitro pseudoneutralisation assays neutralising response, 12.5% 29.5% producing measurable titre doses, A third dose increased...
A key aspect to finding an efficacious human immunodeficiency virus (HIV) vaccine is the optimization of schedules that can mediate efficient maturation protective immune responses. In present study, we investigated effect alternate booster regimens on responses a candidate HIV-1 clade C CN54gp140 envelope protein, which was coadministered with TLR4-agonist glucopyranosyl lipid A-aqueous formulation. Twelve study participants received common three-dose intramuscular priming series followed...
<ns4:p><ns4:italic><ns4:bold>Background</ns4:bold>:</ns4:italic>Human memory B cells play a vital role in the long-term protection of host from pathogenic re-challenge. In recent years importance number different cell subsets that can be formed response to vaccination or infection has started become clear. To study responses, cultured<ns4:italic>ex vivo,</ns4:italic>allowing for an increase and activation these quiescent cells, providing sufficient quantities each subset enable full...
Human memory B cells play a vital role in the long-term protection of host from pathogenic re-challenge. In recent years importance number different cell subsets that can be formed response to vaccination or infection has started become clear. To study responses, cultured
ABSTRACT People living with HIV (PLWH) on suppressive antiretroviral therapy (ART) can have residual immune dysfunction and often display poorer responses to vaccination. We assessed in a cohort of PLWH (n=110) negative controls (n=64) the humoral spike-specific B-cell following 1, 2 or 3 SARS-CoV-2 vaccine doses. had significantly lower neutralizing antibody (nAb) titers than HIV-negative at all studied timepoints. Moreover, their neutralization breadth was reduced fewer individuals...
Immunotherapy has revolutionised cancer therapy but current immune checkpoint inhibitors (ICI) produce low response rates in most cancers, indicating that new therapeutic options are needed. Conventional immune-oncology (IO) discovery uses preclinical models with limited translation capacity as they do not fully recapitulate human tumour complexity. We use multimodal patient molecular data modern machine learning (ML) methods to identify IO targets improved clinical potential. Phrasing...
<title>Abstract</title> Immunotherapy has revolutionised cancer treatment, yet few patients respond clinically, necessitating alternative strategies that can benefit these patients. Novel immune-oncology targets achieve this through bypassing resistance mechanisms to standard therapies. To address this, we introduce MIDAS, a multimodal graph neural network system for target discovery leverages gene interactions, multi-omic patient profiles, immune cell biology, antigen processing, disease...
ABSTRACT Chronic HIV infection drives B-cell dysfunction associated with the accumulation of tissue-like memory (TLMs) and activated B cells (MBCs) but decline in resting cells. TLMs express multiple inhibitory receptors lack response to soluble antigens. However, their origin mechanisms driving expansion remain unclear. By using bulk BCR sequencing cell subsets from elite controllers an ART-controlled cohort, we revealed that (CD21 - CD27 cells) were significantly less mutated also diverse...
Abstract The molecular heterogeneity of autoimmune and inflammatory diseases has been one the main obstacles to development safe specific therapeutic options. Here we have evaluated diagnostic clinical value a robust, inexpensive, immunoassay detecting circulating soluble form monocyte-specific surface receptor sialic acid binding Ig-like lectin 1 (sSIGLEC-1). We developed an measure sSIGLEC-1 in small volumes plasma/serum from systemic lupus erythematosus (SLE) patients healthy donors....
Abstract HIV (human immunodeficiency virus) is a virus that infects different cell types in the immune system. While monoclonal antibodies have been identified, because of antigenic diversity HIV, defining broad neutralising (bnAbs) it has very challenging. Using 10x Chromium technology partitioning system, we performed single-cell sequencing B repertoire and analysed cohort 48 HIV-negative subjects randomised into 4 groups each receiving vaccine regime to investigate type antibody response...
Expression cloning of fully human monoclonal antibodies (hmAbs) is seeing powerful utility in the field vaccinology, especially for elucidating vaccine-induced B-cell responses and novel vaccine candidate antigen discovery. Precision hmAb process relies on efficient isolation hmAb-producing plasmablasts interest. Previously, a immunoglobulin-capture assay (ICA) was developed, using single protein antigens, to enhance pathogen-specific output. Here, we report modification this single-antigen...