A5030027544- Biosensors and Analytical Detection
- Microfluidic and Capillary Electrophoresis Applications
- Microfluidic and Bio-sensing Technologies
- Advanced biosensing and bioanalysis techniques
- Advanced Biosensing Techniques and Applications
- SARS-CoV-2 detection and testing
- Innovative Microfluidic and Catalytic Techniques Innovation
- Nanopore and Nanochannel Transport Studies
- Extracellular vesicles in disease
- Electrowetting and Microfluidic Technologies
- Genomics and Rare Diseases
- Epigenetics and DNA Methylation
- RNA Interference and Gene Delivery
- Advanced Chemical Sensor Technologies
- Cell Image Analysis Techniques
- Probiotics and Fermented Foods
- Respiratory viral infections research
- Genetics, Bioinformatics, and Biomedical Research
- Genomic variations and chromosomal abnormalities
- Single-cell and spatial transcriptomics
- 3D Printing in Biomedical Research
- Cancer Genomics and Diagnostics
- Milk Quality and Mastitis in Dairy Cows
- Mosquito-borne diseases and control
- Cancer Cells and Metastasis
Kwangwoon University
2016-2025
Georgia Institute of Technology
2019-2024
AID Atlanta
2019
Eulji University
2019
Korea Advanced Institute of Science and Technology
2015-2016
Daejeon University
2015
Abstract Extremely rare circulating tumor cell (CTC) clusters are both increasingly appreciated as highly metastatic precursors and virtually unexplored. Technologies primarily designed to detect single CTCs often fail account for the fragility of or leverage cluster-specific markers higher sensitivity. Meanwhile, few technologies targeting CTC lack scalability. Here, we introduce Cluster-Wells, which combines speed practicality membrane filtration with sensitive deterministic screening...
We have developed a simple, user-friendly, and highly sensitive Zika virus (ZIKV) detection method by incorporating optimized reverse transcription loop-mediated isothermal amplification (RT-LAMP) lateral flow assay (LFA). The RT-LAMP reaction was carried out using Bst 3.0 polymerase, which has robust fast performance even in the presence of high concentrations inhibitors; this permitted ZIKV RNA pure water human whole blood. In addition, strong activity polymerase enabled specific without...
A direct integration of the ICP preconcentrator with LFA is simple, cheap, and directly applicable to a commercialized platform.
Gates imprinted on a paper program capillary flow of reagents to create frugal dipstick tests with advanced functionalities.
The global threat posed by the COVID-19 pandemic has catalyzed development of point-of-care (POC) molecular diagnostics. While loop-mediated isothermal amplification (LAMP) stands out as a promising technique among FDA-approved methods, it is occasionally susceptible to high risk false positives due nonspecific primer dimer. In this work, we report an enhancing LAMP in terms assay sensitivity and reliability through streamlined integration with nonpowered nanoelectric preconcentration (NPP)....
Abstract During the pandemic surge, including SARS‐CoV‐2 and influenza, pooling samples emerged as an efficient strategy to identify infected individuals in large groups. While enhances RT‐PCR throughput, reducing costs resources, it dilutes positive with negative ones, lowering sensitivity increasing false negatives. This study proposes a new method address trade‐off between pool sizes accuracy. integrates large‐scale sample enrichment using nano‐hybrid membrane, preventing pooling‐induced...
Severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) is still spreading around the globe causing immense public health and socioeconomic problems. As infection can progress with mild symptoms that be misinterpreted as flu, self-testing methods positively identify SARS-CoV-2 are needed to effectively track prevent transmission of virus. In this work, we report a point-of-care toolkit for multiplex molecular diagnosis influenza A B viruses in saliva samples. Our assay physically...
Abstract Severe acute respiratory syndrome‐coronavirus 2 (SARS‐CoV‐2) continues to threaten lives by evolving into new variants with greater transmissibility. Although lateral flow assays (LFAs) are widely used self‐test for coronavirus disease 2019 (COVID‐19), these tests suffer from low sensitivity leading a high rate of false negative results. In this work, multiplexed assay is reported the detection SARS‐CoV‐2 and influenza A B viruses in human saliva built‐in chemical amplification...
An origami-paper-based device was developed for effective preconcentration and isolation of microvesicles/exosomes by ion concentration polarization.
Ion concentration polarization (ICP) is one of the preconcentration techniques which can acquire a high factor. Still, main hurdles ICP are its instability and low efficiency under physiological conditions with ionic strength abundant biomolecules. Here, we suggested sequentially driven process, enhanced electrokinetic force required for preconcentration, enabling enrichment highly raw samples without increasing electric field. We acquired 13-fold factor (PF) in human serum using paper-based...
Sample preparation steps (e.g., preconcentration and separation) are key to enhancing sensitivity reliability in biomedical analytical chemistry. However, conventional methods ultracentrifugation) cause significant loss of sample as well their contamination. In this study, we developed a paper-based three-dimensional (3D) origami ion concentration polarization preconcentrator (POP) for highly efficient facile preparation. The unique design POP enables simultaneous spatial separation target...
Immunophenotyping is widely used to characterize cell populations in basic research and diagnose diseases from surface biomarkers the clinic. This process usually requires complex instruments such as flow cytometers or fluorescence microscopes, which are typically housed centralized laboratories. Microfluidics combined with an integrated electrical sensor network create antibody microarray for label-free immunophenotyping against multiple antigens. The device works by fractionating sample...
We developed an integrated slidable microdevice which can perform solid phase extraction, μPCR, immunochromatographic strip detection for multiplex pathogen identification on a portable genetic analyzer.
Enzyme-linked immunosorbent assay (ELISA) is widely employed for detecting target molecules in bioassays including the serological assays that measure specific antibody titers. However, ELISA tests are inherently limited to centralized laboratories staffed with trained personnel as workflow requires multiple steps be performed a sequence. Here, we report dipstick test automates this otherwise laborious process and reports titer of molecule digital manner without need an external instrument...
Ion concentration polarization (ICP) has been widely studied for collecting target analytes as it is a powerful preconcentrator method employed charged molecules. Although the quite robust, simple, cheap, and yields high preconcentration factor, major hurdle to be addressed extracting preconcentrated samples without dispersing plug. This study investigates 3D folding-paper-based ICP plug extraction dispersion effect. The printed on cellulose paper with pre-patterned hydrophobic wax. To...
Smith–Kingsmore syndrome (SKS; OMIM 616638), also known as macrocephaly-intellectual disability-neurodevelopmental disorder-small thorax (MINDS; ORPHA 457485), is a rare autosomal dominant disorder, the prevalence of which not known. It caused by heterozygous germline mutation in MTOR (OMIM 601231). Ten different mutations 27 individuals have been reported medical literature to date. These were all gain-of-function missense variants, and about half had c.5395G>A p.(Glu1799Lys) MTOR. Here, I...
Microvesicles and exosomes are small membranous vesicles released to the extracellular environment circulated throughout body. Because they contain various parental cell-derived biomolecules such as DNA, mRNA, miRNA, proteins, lipids, their enrichment isolation critical steps for exploitation potential biomarkers clinical applications. However, conventional methods (e.g., ultracentrifugation) cause significant loss damage microvesicles exosomes. These also require multiple repetitive of...